Structure, antioxidant and immunomodulatory activity of a polysaccharide extracted from Sacha inchi seeds

Tian, Wenni; Xiao, Nan; Yang, Yunyun; Xiao, Jie; Zeng, Renpan; Xie, Lei; Qiu, Ziyou; Li, Pan; Du, Bing

doi:10.1016/j.ijbiomac.2020.06.150

Cited by 25 publications

(12 citation statements)

References 49 publications

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“…Ferreira et al ( 3 ) concluded that elderberry contains glucose, fructose, and a small amount of sucrose. This was similar to the monosaccharide composition of straw mushroom polysaccharides derived by Tian et al ( 47 ), as they are all mainly composed of arabinose, mannose, glucose, and galactose. Data were suggesting that the combinations and ratios of monosaccharides were different, as they were not always the same even in the same genus ( 48 ).…”

Section: Resultssupporting

confidence: 87%

Extraction, isolation, structural characterization, and antioxidant activity of polysaccharides from elderberry fruit

et al. 2022

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The isolation, purification, and antioxidant activity of polysaccharides extracted from elderberry fruits were studied. Two neutral polysaccharides (EFP-0 and EFP-1) and three acidic polysaccharides (EFP-2, EFP-3, and EFP-4) were isolated from elderberry. EFP-0, EFP-1, EFP-2, EFP-3, and EFP-4 all contain arabinose, galactose, glucose, and mannose, with molecular weights of 1.7981 × 106, 7.0523 × 106, 7.7638 × 106, 4.3855 × 105, and 7.3173 × 105 Da, respectively. Structural characterization showed that the backbone of EFP-2 consisted of →4)-Manp (1→4)-β-D-Glcp (1→ and →4)-β-D-Glcp (1→5)-α-L-Araf (1→units, and T-α-L-Araf (1→ and T-β-D-Galp (1→ residues were detected by methylation analysis and NMR analysis. In addition, the MTT assay and zebrafish oxidative damage assay showed that EFP-2 had a protective effect on H2O2-damaged RAW264.7 cells in a dose-dependent manner, and zebrafish with the addition of EFP-2 would have low levels of ROS in vivo which showed significant antioxidant activity. Therefore, the results showed that the elderberry polysaccharides have antioxidant activity and can be used as potential antioxidants in functional foods.

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Section: Resultssupporting

confidence: 87%

Extraction, isolation, structural characterization, and antioxidant activity of polysaccharides from elderberry fruit

et al. 2022

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“…Further studies showed that the iodine‐binding capacity is related to the low‐branching frequency. More importantly, the maximal absorption of DLP‐1 and DLP‐2 with I 2 ‐KI was at 330–360 nm (Figure 3b), implying that DLP‐1 and DLP‐2 might possess longer side chains and branches (Tian et al., 2020).…”

Section: Resultsmentioning

confidence: 99%

“…A previous study had suggested that the high immune activity of D . officinale polysaccharide might be related to its high molar ratio of mannose and the presence of the O‐acetyl group (Tian et al., 2020). The acetyl group in polysaccharides had an impact on the activity of polysaccharides through changing the orientation and horizontal order of polysaccharide molecules and thus altering the physical properties of polysaccharides.…”

Section: Resultsmentioning

confidence: 99%

Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves

Zhong

Tian

Chen

et al. 2021

Journal of Food Biochemistry

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In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP‐1 and DLP‐2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE‐52 cellulose and Sephadex G‐100 columns. They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP‐1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP‐2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%). A triple‐helical conformation was exhibited by both of them. The scanning electron microscope image of DLP‐1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure. In contrast, DLP‐2 revealed a rough, loose, and uneven surface consisting of large sponge‐like particles. Nuclear magnetic resonance analysis showed that (1→4)‐β‐D‐Manp, (1→4)‐β‐D‐Glcp, and (1→4)‐2‐O‐acetyl‐β‐D‐Manp were the main linkage types of DLP‐1, whereas DLP‐2 was constituted by a large amount of (1→4)‐β‐D‐Manp, (1→4)‐β‐D‐Glcp, and other residues. Besides, DLP‐1 and DLP‐2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin‐6, TNF‐α, and IL‐1β. These results proved that both DLP‐1 and DLP‐2 possessed excellent immunoregulatory bioactivities and could be functional food or adjuvant drug. Practical applications The leaf of Dendrobium officinale is a by‐product with huge biomass. The lack of systematic research on its chemical composition and pharmacologic effect, leading to a great waste of resources. In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP‐1 and DLP‐2) from D. officinale leaves, showing that DLP‐1 and DLP‐2 in D. officinale leaves could be used as anti‐inflammatory agents to avoid wasting.

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“…Figure 3 shows the 1 H (A), 13 C (B), DEPT (C), HSQC (D) and COSY (E) spectrums of LP4. The chemical shifts at δ 3.20-δ 5.50 ppm in 1 H NMR spectrum were the signals of polysaccharide and the signals in δ 4.4-5.8 ppm region represented the anomer of sugar residue (Song et al, 2019;Tian et al, 2020). Ordinarily, the signals at δ 4.4-5.0 ppm represent β-configuration and α-configuration distributed in δ 5.0-5.8 ppm (Liu et al, 2018).…”

Section: Nmr Analysismentioning

confidence: 99%

A Novel Polysaccharide Isolated From Fresh Longan (Dimocarpus longan Lour.) Activates Macrophage via TLR2/4-Mediated PI3/AKT and MyD88/TRAF6 Pathways

Lan

et al. 2021

Front. Pharmacol.

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A novel immunomodulatory polysaccharide (LP4) with a molecular weight 6.31 × 104 g/mol was purified from fresh longan pulp. It was composed of mannose, glucose, glucuronic acid, galactose, xylose, arabinose, galacturonic acid, fucose, and rhamnose in a molar percentage of 36:31:10:7:4:4:3:2:2, and mainly linked by (1→6)-β-Man, (1→4)-β-Glc and (1→6)-α-Glc. LP4 can obviously enhance the phagocytosis of macrophages and promote the proliferation of lymphocytes. After treating macrophages with LP4 (12.5–50 μg/ml), the production of IL-1β and TNF-α was significantly increased. These increases of cytokines were suppressed when the TLR2/TLR4 receptors were inhibited by anti-TLR2 and/or anti-TLR4 antibodies. Moreover, the mRNA expression of INOS, AKT, PI3K, TRAF6 and MyD88 was significantly suppressed by TLR2/TLR4 antibodies. These results indicated that LP4 induced macrophage activation mainly via the TLR2 and TLR4-induced PI3K/AKT and MyD88/TRAF6 pathways.

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Structure, antioxidant and immunomodulatory activity of a polysaccharide extracted from Sacha inchi seeds

Cited by 25 publications

References 49 publications

Extraction, isolation, structural characterization, and antioxidant activity of polysaccharides from elderberry fruit

Extraction, isolation, structural characterization, and antioxidant activity of polysaccharides from elderberry fruit

Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves

A Novel Polysaccharide Isolated From Fresh Longan (Dimocarpus longan Lour.) Activates Macrophage via TLR2/4-Mediated PI3/AKT and MyD88/TRAF6 Pathways

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