Structure and ultrastructure of spermatozoa in Meliponini (stingless bees) (Hymenoptera: Apidae)

Zama, Uyrá; Lino‐Neto, José; Dolder, Heidi

doi:10.1016/j.tice.2003.08.003

Cited by 32 publications

(78 citation statements)

References 22 publications

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“…The structure of the H. foveolatum spermatozoa is similar to the general description for insect sperm ( Quicke et al, 1992, Newman and Quicke, 1998, Newman and Quicke, 1999a, Newman and Quicke, 1999b, Newman and Quicke, 2000, Lino-Neto et al, 1999, Lino-Neto et al, 2000a, Lino-Neto et al, 2000b, Lino-Neto and Dolder, 2001a, Lino-Neto and Dolder, 2001b, Lino-Neto and Dolder, 2002, Zama et al, 2001, Zama et al, 2004, Zama et al, 2005a, Zama et al, 2005b, Báo et al, 2004and Fiorillo et al, 2005. They are linear, long and slender as which is observed for the majority of the Hymenoptera.…”

Section: Discussionsupporting

confidence: 67%

“…The mature nucleus presenting a loose chromatin differs from the Hymenoptera pattern, but it was also observed in Halictinae (Fiorillo et al, 2005), and in some Meliponini (Zama et al, 2004). The nuclear base penetrating into the beginning of the axoneme has also been observed in Apinae (Peng et al, 1993 andLino-Neto et al, 2000b) and Vespidae (Mancini et al, 2006).…”

Section: Discussionmentioning

confidence: 90%

“…The nuclear base penetrating into the beginning of the axoneme has also been observed in Apinae (Peng et al, 1993 andLino-Neto et al, 2000b) and Vespidae (Mancini et al, 2006). However, it is uncommon for the vast majority of Apoidea, which have the nuclear base finishing in a short cone above the axonemal implantation (Zama et al, 2001, Zama et al, 2004, Zama et al, 2005a, Zama et al, 2005b, Báo et al, 2004and Fiorillo et al, 2005.…”

Section: Discussionmentioning

confidence: 93%

“…It was described for Symphyta (Quicke et al, 1992 andQuicke, 1999a), parasitic wasps (Lino-Neto and Dolder, 2001a), ants (Wheeler et al, 1990 andDolder, 2002) and for bees (Peng et al, 1993, Zama et al, 2001, Zama et al, 2004, Zama et al, 2005a, Zama et al, 2005b, Báo et al, 2004and Fiorillo et al, 2005. The presence of four acrosomal vesicle lateral projections along the perforatorium is not observed in other Hymenoptera already examined.…”

Section: Discussionmentioning

confidence: 99%

“…Some recent studies have demonstrated that the structure and ultrastructure of spermatozoa in Hymenoptera furnish sufficient variations to provide additional characters for cladistic analysis (Quicke et al, 1992, Dallai and Afzelius, 1995, Lino-Neto et al, 1999, Lino-Neto et al, 2000a, Lino-Neto et al, 2000b, Lino-Neto and Dolder, 2001a, Lino-Neto and Dolder, 2001b, Lino-Neto and Dolder, 2002, Zama et al, 2001, Zama et al, 2004, Zama et al, 2005a, Zama et al, 2005b, Zama et al, 2007, Báo et al, 2004, Araújo et al, 2005and Fiorillo et al, 2005 as has been shown possible in other groups of insects (Baccetti, 1987 andJamieson et al, 1999). The study of spermiogenesis can also provide new characters for improving the accuracy of hypotheses on phylogenetic relationships.…”

Section: Introductionmentioning

confidence: 99%

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Morphology of the male reproductive system and spermiogenesis in Hypanthidium foveolatum (Alfken, 1930) (Hymenoptera: Apidae: Megachilinae)

Gracielle

Fiorillo

Lino‐Neto

et al. 2009

Micron

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The morphological aspects of male reproductive tract, spermiogenesis and spermatozoa are typical for each species and reflect its evolution, establishing a unique source of characters, which has been used to help solve phylogenetic problems. In Hypanthidium foveolatum the reproductive tract is composed of the testes comprising 28 testicular tubules, deferent ducts, seminal vesicles, accessory glands and an ejaculatory duct. The differentiation of spermatids occurs within cysts of up to 128 germ line cells each one. During the early spermatid phase, the nucleus resembles that of somatic cells. There follows a gradual chromatin condensation with an increase in nuclear electron density. In the spermatozoon, the nucleus contains heterogeneous chromatin with a loose appearance. The acrosome, shaped with the active participation of the Golgi complex, shows an electron-dense perforatorium involved by four electron-lucent acrosomal vesicle projections. The sperm tail presents an axoneme with a 9 + 9 + 2 microtubule pattern and two mitochondrial derivatives, which appear with different sizes. A dense crystalloid is formed initially in the mitochondrial matrix of the large derivative. The mitochondrial derivatives' differentiation occurs concomitantly with an axoneme outgrowth. The centriolar adjunct is observed near the axoneme, anterior to the smaller mithocondrial derivative and exhibits an approximately triangular shape in cross-sections. Microtubules were observed around the head region and flagellar components during spermiogenesis.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Morphology of the male reproductive system and spermiogenesis in Hypanthidium foveolatum (Alfken, 1930) (Hymenoptera: Apidae: Megachilinae)

Gracielle

Fiorillo

Lino‐Neto

et al. 2009

Micron

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Ultrastructure of spermatozoa in two solitary bee species with an emphasis on synapomorphic traits shared in the family apidae

Araújo

Serrão

Moreira

et al. 2012

Microscopy Res & Technique

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Morphology of spermatozoa in bees has provided promising results for phylogenetic analyses. In this work, the structure and ultrastructure of spermatozoa from Thygater (Thygater) analis and Melitoma segmentaria were characterized and the synapomorphies shared in the family Apidae are discussed. In these species, spermatozoa bundles which are undone in the seminal vesicle possess, on average, 50 cells. Spermatozoa consist of a head and a flagellar region. The head includes an acrosome containing the perforatorium, covered by the acrosomal vesicle and a nucleus. The flagellum is formed by two mitochondrial derivatives, which are asymmetric in diameter and length, with one centriolar adjunct, one axoneme (9 + 9 + 2), and two accessory bodies. In cross section the centriolar adjunct is asymmetric and the accessory bodies are triangular in shape. In the distal region of the flagellum, the derivative terminates before the axoneme and the small derivative terminates first. The axoneme is gradually disorganized and the accessories microtubules are the last to terminate. In these two species, spermatozoa share diverse synapomorphies with those of other bee species previously described in the literature, which allows for the establishment of a morphological pattern for spermatozoa of the family Apidae.

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Ultrastructural characterization of spermatozoa in euglossine bees (Hymenoptera, Apidae, Apinae)

et al. 2005

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Euglossine spermatozoa are the longest described to date for the Hymenoptera. This cell includes a head and a flagellar region. In transverse sections, the acrosome is circular at the tip but has an oval contour along most of its length. The perforatorium penetrates into a deep cavity in the nuclear tip. The flagellum consists in an axoneme, a pair of mitochondrial derivatives, a centriolar adjunct and a pair of accessory bodies. The axoneme has a 9+9+2 microtubule pattern which becomes gradually disorganized in the final portion, with the central microtubules and the nine doublets terminating simultaneously, followed by the accessory microtubules. The mitochondrial derivatives are asymmetric both in length and diameter. Sectioned transversally, the derivatives are ellipsoidal or have a pear shape. The larger one has a more obvious paracrystalline region. The centriolar adjunct begins at the nuclear base and extends parallel to the axoneme until it encounters the smaller mitochondrial derivative, on which it fits, making a concave groove. In addition to these consistent euglossine features, species-specific differences that might be useful in phylogenetic work on the group are also noted.

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Structure and ultrastructure of spermatozoa in Meliponini (stingless bees) (Hymenoptera: Apidae)

Cited by 32 publications

References 22 publications

Morphology of the male reproductive system and spermiogenesis in Hypanthidium foveolatum (Alfken, 1930) (Hymenoptera: Apidae: Megachilinae)

Morphology of the male reproductive system and spermiogenesis in Hypanthidium foveolatum (Alfken, 1930) (Hymenoptera: Apidae: Megachilinae)

Ultrastructure of spermatozoa in two solitary bee species with an emphasis on synapomorphic traits shared in the family apidae

Ultrastructural characterization of spermatozoa in euglossine bees (Hymenoptera, Apidae, Apinae)

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