Structure and synthesis of a lipid-containing bacteriophage

Tsukagoshi, Norihiro; Petersen, Marianne H.; Franklin, Richard M.

doi:10.1016/0042-6822(75)90191-9

Cited by 13 publications

(22 citation statements)

References 20 publications

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“…In general, the fatty acid composition of the virion is similar to that of the host cell with some slight differences [5,6]. It is, therefore, possible to modify the fatty acid composition of the virion by varying the fatty acid in the growth medium and thereby varying the fatty acid composition of the host cell.…”

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confidence: 95%

“…Pseudomonas BAL-31 was grown at 25 "C in AA medium which contains BS salts consisting of NaCl, 12.12 g Tris-HC1 and 2.78 g Tris base per liter and 2 g per liter of a mixture of twenty amino acids [1,6]. The isolation of the fatty acid auxotroph, strain UFA, has been described by Tsukagoshi et al [1,6].…”

Section: Growth Of Bacteriamentioning

confidence: 99%

“…The isolation of the fatty acid auxotroph, strain UFA, has been described by Tsukagoshi et al [1,6]. Strain UFA was grown at 25 "C in AA medium supplemented with 0.01 % of an essential fatty acid.…”

Section: Growth Of Bacteriamentioning

confidence: 99%

“…In order to study the effect of host cell lipids on the lipid composition in virions, an unsaturated fatty acid auxotroph, strain UFA, has been isolated from Pseudomonas BAL-31 [5,6]. In general, the fatty acid composition of the virion is similar to that of the host cell with some slight differences [5,6].…”

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confidence: 99%

“…The virus, which has a lipid bilayer [2], matures at the periphery of the host bacterium, but not in association with the host cell membrane [3]. One third of the viral lipids are derived from lipids synthesized before infection and two thirds from lipids synthesized during viral maturation [4].…”

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confidence: 99%

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Phase Transitions in the Membrane of a Marine Bacterium, Pseudomonas BAL‐31

Tsukagoshi

Petersen

Huber

et al. 1976

European Journal of Biochemistry

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An unsaturated fatty acid auxotroph, strain UFA, isolated from the marine pseudomonad Pseudomonas BAL‐31, host cell of the lipid‐containing bacteriophage PM2, was grown in media supplemented with different unsaturated fatty acids. Under these conditions the fatty acid composition of the cell could be altered drastically. The phase transition in the native membrane and in the extracted lipids was analyzed by electron spin resonance using a nitroxide spin probe. Membranes prepared from strain UFA grown in cisl6: 1 or trans16:1 showed one transition at 9.4 °C and 12.4 °C respectively. Extracted lipids in both cases had almost the same transition temperature as that of the intact membrane. Membranes prepared from Pseudomonas BAL‐31 had one transition at ∼ 12 °C, on the other hand there was no clear cut phase transition using extracted lipids. Replication of bacteriophage PM 2 took place below the transition temperature of the membrane lipids in the case where strain UFA was grown in trans 16: 1. Other cases were not studied.

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confidence: 95%