Protein and peptide assembly into amyloid has been implicated in functions that range from beneficial epigenetic controls to pathological etiologies. However, the exact structures of the assemblies that regulate biological activity remain poorly defined. We have previously used Zn 2؉ to modulate the assembly kinetics and morphology of congeners of the amyloid  peptide (A) associated with Alzheimer's disease. We now reveal a correlation among A-Cu 2؉ coordination, peptide self-assembly, and neuronal viability. By using the central segment of A, HHQKLVFFA or A(13-21), which contains residues H13 and H14 implicated in A-metal ion binding, we show that Cu 2؉ forms complexes with A(13-21) and its K16A mutant and that the complexes, which do not selfassemble into fibrils, have structures similar to those found for the human prion protein, PrP. N-terminal acetylation and H14A substitution, Ac-A(13-21)H14A, alters metal coordination, allowing Cu 2؉ to accelerate assembly into neurotoxic fibrils. These results establish that the N-terminal region of A can access different metal-ion-coordination environments and that different complexes can lead to profound changes in A self-assembly kinetics, morphology, and toxicity. Related metal-ion coordination may be critical to the etiology of other neurodegenerative diseases.copper-binding ͉ neurotoxicity ͉ self-assembly P rotein intermolecular assembly, especially formation of amyloid fibrillar structures, is correlated with a variety of human neurodegenerative diseases, including Alzheimer's, Parkinson's, Huntington's, and Creutzfeldt-Jakob diseases (1). More recently, amyloid has been tied to many nonpathological functional roles. For example, formation and self-perpetuation of amyloids in Saccharomyces cerevisiae regulate diverse yeast phenotypic expression as a positive response to environmental fluctuations (2), and amyloid may be involved in long-term memory and synapse maintenance in the marine snail, Aplysia (3, 4). Many proteins, including archetypical globular proteins such as myoglobin, can also form amyloid fibrils, suggesting that amyloidogenesis may be an intrinsic property of any ␣-amino acid polymer (5). Accordingly, these highly ordered paracrystalline protein self-assemblies have now been recognized as useful for nanostructure fabrication and biotechnology (6-8). Fully capturing these technological opportunities and understanding the biological roles of amyloid will depend on further definition of the organized structure and assembly pathway.Increasing evidence now implicates transition metal ions, including Zn 2ϩ , Cu 2ϩ , and Fe 3ϩ , as contributors both to amyloid  (A) assembly in vitro and to the neuropathology of Alzheimer's disease, AD (9). The obligatory region of metal ion (Zn 2ϩ /Cu 2ϩ ) binding of A has been mapped to the N terminus, amino acids 1-28 (10-16). In its soluble nonamyloid conformation, the peptide contains multiple intramolecular binding sites for Zn 2ϩ and Cu 2ϩ (9, 17), and intermolecular Zn 2ϩ binding can promote A aggrega...