Structure and Stability of Complex Coacervate Core Micelles with Lysozyme

Lindhoud, Saskia; Vries, Renko de; Stuart, Martien A. Cohen

doi:10.1021/bm0700688

Cited by 81 publications

(129 citation statements)

References 33 publications

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“…Besides co-micellization with DNA and RNA, other biopolymers, such as proteins, peptides, and enzymes, may also be encapsulated to preserve and/or promote stability and function, as was demonstrated for KALA, [88,140] lysozyme, [121,122,[141][142][143][144][145][146]150] trypsin, [148,149] myoglobin, [104] and BSA [62]. Circular dichroism (CD) experiments on lysozyme-containing C3Ms demonstrated that the secondary structure of free and C3M incorporated lysozyme is identical [144].…”

Section: Comicellization With Proteins Peptides and Enzymesmentioning

confidence: 99%

“…The critical ionic strength can be determined from the inclination point in a plot of the (initially decreasing) static light scattering intensity upon increasing ionic strength, due to a decrease in particle mass and aggregation number upon micellar dissociation until the critical ionic strength is reached and no more C3Ms can be observed [29,96,100,150]. Similarly, the critical pH for C3M formation in systems wherein at least one component is a weak polyelectrolyte, can be determined from a plot of the static light scattering intensity versus pH [100,252].…”

Section: Static Light Scattering (Sls)mentioning

confidence: 99%

“…For example, R h was found to pass through a maximum at (near) isoelectric conditions for C3Ms of PTEA-b-PAAm and CeO 2 or γ-Fe 2 O 3 nanoparticles [223]. Research in our laboratory on the effect of the mixing fraction, pH, and ionic strength on micellar formation and properties, has benefited greatly from the combination of an automated titration and (static and dynamic) light scattering setup [44,45,94,95,100,150].…”

Section: Dynamic Light Scattering (Dls)mentioning

confidence: 99%

“…The critical ionic strength is strongly dependent on the type of oppositely charged species, solution pH, mixing fraction, micellar concentration, and valency and type of added salt [130,131]. For addition of monovalent salts under charge stoichiometric conditions (see also Sections 5.2 and 5.3), the I cr may vary from 50-600 mM for C3Ms consisting of solely linear synthetic polymers, [3,44,95,100] and synthetic polymers and proteins, [122,150] up to 1-2.8 M for micelles formed through a combination of driving forces, such as electrostatic and hydrophobic interaction [45] or metal-coordination [152,[154][155][156]. Nanoparticle-containing C3Ms generally exhibit a higher I cr than C3Ms consisting of solely linear synthetic polymers.…”

Section: Effect Of Ionic Strengthmentioning

confidence: 99%

See 3 more Smart Citations

Complex coacervate core micelles

Voets¹,

Keizer²,

Stuart³

2009

Advances in Colloid and Interface Science

380

484

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Section: Comicellization With Proteins Peptides and Enzymesmentioning

confidence: 99%

Section: Static Light Scattering (Sls)mentioning

confidence: 99%

Section: Dynamic Light Scattering (Dls)mentioning

confidence: 99%

Section: Effect Of Ionic Strengthmentioning

confidence: 99%

See 2 more Smart Citations

Complex coacervate core micelles

Voets¹,

Keizer²,

Stuart³

2009

Advances in Colloid and Interface Science

380

484

View full text Add to dashboard Cite

“…However, for the other ingredient(s) one has quite some freedom. Simple polyelectrolytes of varying length have been used, but also proteins (enzymes) [23], ionic surfactants [24], and inor- Fig. 9 Symmetry breaking in a micelle with a mixed corona, as modeled by means of selfconsistent field calculations [19] imposing a non-deformable spherical core.…”

Section: Hierarchical Self-assemblymentioning

confidence: 99%

Supramolecular perspectives in colloid science

Stuart

2008

Colloid Polym Sci

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Supramolecular chemistry puts emphasis on molecular assemblies held together by non-covalent bonds. As such, it is very close in spirit to colloid science which also focuses on objects which are small, but beyond the molecular scale, and for which other forces than covalent bonds are crucial. We discuss in this review the preparation and properties of new colloidal systems which borrow on the one hand from classical topics in colloid science, such as micellization, and on the other hand from concepts in supramolecular chemistry, such as reversible supramolecular polymers.

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Biopolymeric Coacervate Microvectors for the Delivery of Functional Proteins to Cells

et al. 2020

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Although protein therapeutics have exceptional potential for nextgeneration therapies, delivery of these macromolecules is hindered due to their vulnerability to biological deactivation, [6-9] poor native membrane permeability, and short circulation time if utilized intravenously. [10-12] For these reasons, the development of protein delivery technologies is a vital endeavor that promises to unlock the potential of this significant class of bioactive macromolecules. [13,14] An emerging strategy to address the challenge of protein delivery is via the use of coacervate particles, which is appealing due to their ability to spontaneously sequester various macromolecular cargoes, [15,16] and to undergo direct cellular interactions. [17] In these terms, coacervates have been described as biomimetic microcontainers due to their physicochemical semblance to the cell cytoplasm, albeit without an external membrane. [18,19] Although being a relative newcomer to the field of therapeutic protein delivery, coacervate-based materials have received significant attention in biomedical research and have already been used for drug delivery. [20,21] For example, coacervate-based materials have shown great promise for controlled growth factor delivery in vivo for attenuation of disc degeneration, persistent angiogenesis, preservation of The extent to which biologic payloads can be effectively delivered to cells is a limiting factor in the development of new therapies. Limitations arise from the lack of pharmacokinetic stability of biologics in vivo. Encapsulating biologics in a protective delivery vector has the potential to improve delivery profile and enhance performance. Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids. Here, the development of biodegradable coacervate microvectors (comprising synthetically modified amylose polymers) is presented, for the delivery of biologic payloads to cells. Amylose-based coacervate microdroplets are stable under physiological conditions (e.g., temperature and ionic strength), are noncytotoxic owing to their biopolymeric structure, spontaneously interacted with the cell membrane, and are able to deliver and release proteinaceous payloads beyond the plasma membrane. In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells. This study demonstrates the potential of coacervate microdroplets as delivery microvectors for biomedical research and the development of new therapies.

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Structure and Stability of Complex Coacervate Core Micelles with Lysozyme

Cited by 81 publications

References 33 publications

Complex coacervate core micelles

Complex coacervate core micelles

Supramolecular perspectives in colloid science

Biopolymeric Coacervate Microvectors for the Delivery of Functional Proteins to Cells

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