Structure and Sequence of the Sex Determining Locus in Two Wild Populations of Nile Tilapia

Triay, Cécile; Conte, Matthew A.; Baroiller, Jean‐François; Bezault, Étienne; Clark, Frances E.; Penman, David J.; Kocher, Thomas D.; D'Cotta, Hélèna

doi:10.3390/genes11091017

Cited by 18 publications

(31 citation statements)

References 76 publications

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“…One of these loci, an XY system determiner on linkage group 23, contains a duplication of the amh gene and subsequent smaller deletion that is male-specific in some lineages [Wessels et al, 2017] and variable in wild populations [Sissao et al, 2019;Triay et al, 2020]. The typically male-biased amh gene has previously shown to also be upregulated in sex-reversed neomales along with the dmrt1 gene, followed in time by reduced expression of the typically female-biased foxl1 and cyp19a1a (gonad form) as well as downregulation of cyp19a1b (brain form) [Poonlaphdecha et al, 2013].…”

Section: Mechanisms Of Esd In Cichlidsmentioning

confidence: 99%

Epigenetic Regulation and Environmental Sex Determination in Cichlid Fishes

Renn

Hurd

2021

Sex Dev

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Studying environmental sex determination (ESD) in cichlids provides a phylogenetic and comparative approach to understand the evolution of the underlying mechanisms, their impact on the evolution of the overlying systems, and the neuroethology of life history strategies. Natural selection normally favors parents who invest equally in the development of male and female offspring, but evolution may favor deviations from this 50:50 ratio when environmental conditions produce an advantage for doing so. Many species of cichlids demonstrate ESD in response to water chemistry (temperature, pH, and oxygen concentration). The relative strengths of and the exact interactions between these factors vary between congeners, demonstrating genetic variation in sensitivity. The presence of sizable proportions of the less common sex towards the environmental extremes in most species strongly suggests the presence of some genetic sex-determining loci acting in parallel with the ESD factors. Sex determination and differentiation in these species does not seem to result in the organization of a final and irreversible sexual fate, so much as a life-long ongoing battle between competing male- and female-determining genetic and hormonal networks governed by epigenetic factors. We discuss what is and is not known about the epigenetic mechanism behind the differentiation of both gonads and sex differences in the brain. Beyond the well-studied tilapia species, the 2 best-studied dwarf cichlid systems showing ESD are the South American genus <i>Apistogramma</i> and the West African genus <i>Pelvicachromis</i>. Both species demonstrate male morphs with alternative reproductive tactics. We discuss the further neuroethology opportunities such systems provide to the study of epigenetics of alternative life history strategies and other behavioral variation.

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Section: Mechanisms Of Esd In Cichlidsmentioning

confidence: 99%

Epigenetic Regulation and Environmental Sex Determination in Cichlid Fishes

Renn

Hurd

2021

Sex Dev

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“…Genetic markers associated with sex determination have been successfully detected on ChrLG1 ( O. niloticus ), ChrLG3 ( O. karongae , Tilapia mariae, and O. aureus ), and ChrLG23 (a cross between red tilapia and O. niloticus ) in various tilapia ( Cnaani et al, 2008 ; Cnaani et al, 2004 ; Ezaz et al, 2004 ; Gammerdinger et al, 2016 ; Lee et al, 2004 ; Lee et al, 2005 ; Liu et al, 2013 ). ChrLG3 has been considered as a potential sex chromosome due to the abnormal morphology of chromosomes in blue and Nile tilapia ( Cnaani et al, 2008 ; Triay et al, 2020 ). Recent progress in quantitative trait loci (QTL) mapping with high-throughput sequencing data suggests there are genome-wide significant QTL controlling sex on ChrLG1 (10.1–18.9 Mb), ChrLG3 (40–80 Mb), ChrLG20 (12.6–29.1 Mb), ChrLG22 and ChrLG23 (24–40 Mb) in Nile tilapia ( Gammerdinger et al, 2016 ; Palaiokostas et al, 2013 ; Triay et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…ChrLG3 has been considered as a potential sex chromosome due to the abnormal morphology of chromosomes in blue and Nile tilapia ( Cnaani et al, 2008 ; Triay et al, 2020 ). Recent progress in quantitative trait loci (QTL) mapping with high-throughput sequencing data suggests there are genome-wide significant QTL controlling sex on ChrLG1 (10.1–18.9 Mb), ChrLG3 (40–80 Mb), ChrLG20 (12.6–29.1 Mb), ChrLG22 and ChrLG23 (24–40 Mb) in Nile tilapia ( Gammerdinger et al, 2016 ; Palaiokostas et al, 2013 ; Triay et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…The first SD gene reported in fish, Dmy , is required for normal male development in medaka ( Matsuda et al, 2002 ). In tilapia, amhy , a Y-specific duplicate of the anti-Müllerian hormone ( amh ) gene, regulates male development in Nile tilapia ( Li et al, 2015 ; Taslima et al, 2020 ; Triay et al, 2020 ). Recently, several sex-related genes, such as tsp1a , have been implicated in female folliculogenesis development in Nile tilapia ( Jie et al, 2020 ), and Paics shows female-specific patterns during early gonadogenesis ( Tao et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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First identification of two co-existing genome-wide significant sex quantitative trait loci (QTL) in red tilapia using integrative QTL mapping

Zhu¹,

Lin²,

Ai³

et al. 2022

Zoological Research

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Red tilapia ( Oreochromis spp . ) is one of the most popular fish in China due to its bright red appearance, fast growth rate, and strong adaptability. Understanding the sex determination mechanisms is of vital importance for the selection of all-male lines to increase aquacultural production of red tilapia. In this research, the genetic architecture for sex from four mapping populations ( n =1 090) of red tilapia was analyzed by quantitative trait loci (QTL)-seq, linkage-based QTL mapping, and linkage disequilibrium (LD)-based genome-wide association studies. Two genome-wide significant QTL intervals associated with sex were identified on ChrLG1 (22.4–23.9 Mb) and ChrLG23 (32.0–35.9 Mb), respectively. The QTL on ChrLG1 was detected in family 1 (FAM1), FAM2, and FAM4, and the other QTL on ChrLG23 was detected in FAM3 and FAM4. Four microsatellite markers located within the QTL were successfully developed for marker-assisted selection. Interestingly, three ( lpp , sox14 , and amh ) of the 12 candidate genes located near or on the two QTL intervals were abundantly expressed in males, while the remaining genes were more highly expressed in females. Seven genes ( scly , ube3a , lpp , gpr17 , oca2 , cog4 , and atp10a ) were significantly differentially expressed between the male and female groups. Furthermore, LD block analysis suggested that a cluster of genes on ChrLG23 may participate in regulating sex development in red tilapia. Our study provides important information on the genetic architecture of sex in red tilapia and should facilitate further exploration of sex determination mechanisms in this species.

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“…The various genomic approaches of quantitative trait loci (QTL) and physical mapping have been carried out to identify QTL for sex determination in Nile tilapia populations (Eshel et al, 2012;Palaiokostas et al, 2013;Sun et al, 2014). Currently, the genome-wide association study (GWAS) and whole-genome sequencing (WGS) have been used to identify candidate genes for sex determination in Nile tilapia (Cáceres et al, 2019;Triay et al, 2020). Numerous strong candidate genes for involvement in sex determination of Nile tilapia have been described e.g., anti-Müllerian hormone (Amh), doublesex-and mab-3 related transcription factor 2 (Dmrt2), Wilms tumor suppressor protein 1b (WT1b), and cytochrome P450 family 19 subfamilies A member 1 (CYP19A1) genes (Shirak et al, 2006;Lee and Kocher, 2007).…”

Section: Introductionmentioning

confidence: 99%

Association of genetic markers with sex determination in Thai red tilapia

Naraballobh

Pothakam²,

Norseeda

et al. 2021

VIS

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The objectives of this study were to verify the polymorphism on sex-linked marker loci and to assess their associations with phenotypic sex characteristics in red tilapia. Four sex-linked genetic markers of Amh, SCAR4, SCAR5, and Oni3161 were genotyped in the Thai red tilapia population. The Amh marker was significantly associated with the phenotypic sex of red tilapia with an accuracy of 46.2%. No significant association of SCAR4, SCAR5, and Oni3161 marker polymorphisms with phenotypic sex characteristics was observed in this study. However, the combinations of these two, three, and four markers were increasingly associated with phenotypic sex characteristics for red tilapia with an accuracy of 62.8, 68.4, and 72.4%, respectively. These results indicate that these combined genetic markers were associated with the phenotypic sex of red tilapias. These findings confirmed the importance of these genetic markers as candidate markers for sex determination in the Thai red tilapia population.

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Structure and Sequence of the Sex Determining Locus in Two Wild Populations of Nile Tilapia

Cited by 18 publications

References 76 publications

Epigenetic Regulation and Environmental Sex Determination in Cichlid Fishes

Epigenetic Regulation and Environmental Sex Determination in Cichlid Fishes

First identification of two co-existing genome-wide significant sex quantitative trait loci (QTL) in red tilapia using integrative QTL mapping

Association of genetic markers with sex determination in Thai red tilapia

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