Structure and RFLP mapping of a rice sucrose phosphate synthase (SPS) gene that is specifically expressed in the source organ

Sakamoto, Masahiro; Satozawa, Tomomi; Higo, K.; Shimada, Hiroaki; Fujimura, Tatsuhito

doi:10.1016/0168-9452(95)04263-6

Cited by 14 publications

(8 citation statements)

References 31 publications

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“…The regulation includes organ-specific expression (Cpsps2 is not present in roots) and modulation by the water status of the tissue. Organ-specific expression of SPS transcripts has also been reported for other plant species: the SPS transcripts were detected mainly in leaves of spinach or rice Sakamoto et al, 1995) or in roots of sugar beet (Hesse et al, 1995). The transcript distribution in C. plantagineum is different: distinct SPS transcripts are present in leaves, roots, and callus.…”

Section: Regulation Of Sps Cene Expressionmentioning

confidence: 80%

“…To analyze the expression of SPS at the protein level, polyclonal antibodies were generated (for details, see "Ma- (Komatsu et al, 1996), sugar beet (Hesse et al, 1995), spinach (Sonnewald et al, 19931, maize (Worrell et al, 1995), and rice (Sakamoto et al, 1995). The comparison was produced using the PILEUP program of the University of Wisconsin Genetics Computer Group.…”

Section: Sps Protein Expressionmentioning

confidence: 99%

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Analysis of cDNA Clones Encoding Sucrose-Phosphate Synthase in Relation to Sugar Interconversions Associated with Dehydration in the Resurrection Plant Craterostigma plantagineum Hochst

Ingram¹,

Chandler²,

Gallagher³

et al. 1997

Plant Physiology

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Sucrose-phosphate synthase (SPS) is a key enzyme in the regulation of sucrose metabolism, being responsible for the synthesis of sucrose 6-phosphate from fructose 6-phosphate and uridine 5 ' -diphosphate-glucose. W e report on the isolation and characterization of cDNA clones encoding SPS from Craferosfigma planfagineum Hochst., a resurrection plant in which the accumulation of sucrose is considered to play an important role in tolerance to severe protoplastic dehydration. Two distinct classes of cDNAs encoding SPS were isolated from C. plantagineum, and are represented by the clones Cpspsl and CpspsZ. l h e transcripts corresponding to both cDNAs decrease to very low levels in dehydrating leaves of C. plantagineum. Only the Cpspsl transcript occurs in the roots, where it is present at a higher leve1 than in leaves and increases upon dehydration of the plant. Higher enzymatic activities have been determined in protein extracts of dehydrated tissues compared with untreated tissues, which correlates with an increase in protein levels. It is suggested that the overall regulation of SPS is strongly influenced by the changing composition of the cytoplasm in C. plantagineum leaves during the dehydration-rehydration cycle.

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Section: Regulation Of Sps Cene Expressionmentioning

confidence: 80%

Section: Sps Protein Expressionmentioning

confidence: 99%

Analysis of cDNA Clones Encoding Sucrose-Phosphate Synthase in Relation to Sugar Interconversions Associated with Dehydration in the Resurrection Plant Craterostigma plantagineum Hochst

Ingram¹,

Chandler²,

Gallagher³

et al. 1997

Plant Physiology

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“…However, Sakamoto et al (1995) reported the map-based cloning of a single SI'S gene in rice source organs, and Worrell et al (1991) reported that S E enzyme activity was conferred by a single gene product in maize. To date, no evidence has been presented to our knowledge for the occurrence of spinach-leaf SPS isoforms that differ in molecular weight from the native enzyme under conditions of abiotic stress (for review, see Guy et al [1992]).…”

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confidence: 99%

Protein Phosphorylation as a Mechanism for Osmotic-Stress Activation of Sucrose-Phosphate Synthase in Spinach Leaves

1997

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Experiments were performed to investigate the mechanism of sucrose-phosphate synthase (SPS) activation by osmotic stress i n darkened spinach (Spinacia oleracea L.) leaves. The activation was stable through immunopurification and was not the result of an increased SPS protein level. l h e previously described Ca2+-independent peak III kinase, obtained by ion-exchange chromatography, is confirmed to be the predominant enzyme catalyzing phosphorylation and inactivation of dephosphoserine-158-SPS. A new, Ca'+-dependent SPS-protein kinase activity (peak IV kinase) was also resolved and shown to phosphorylate and activate phosphoserine-158-SPS i n vitro. The peak I V kinase also phosphorylated a synthetic peptide (SP29) based on the amino acid sequence surrounding serine-424, which also contains the motif described for the serine-158 regulatory phosphorylation site; i.e. basic residues at P-3 and P-6 and a hydrophobic residue at P-5. Peak I V kinase had a native molecular weight of approximately 150,000 as shown by gel filtration. The SP29 peptide was not phosphorylated by the inactivating peak III kinase. Osmotically stressed leaves showed increased peak I V kinase activity with the SP29 peptide as a substrate. l r y p t i c 32P-phosphopeptide analysis of SPS from excised spinach leaves fed [32P]inorganic P showed increased phosphorylation of the tryptic peptide containing serine-424. Therefore, at least part of the osmotic stress activation of SPS i n dark leaves results from phosphorylation of serine-424 catalyzed by a Ca'+-dependent, 150-kD protein kinase.

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“…The rice sps1 gene was also mapped on rice chromosome 1 and corresponded to maize chromosome bin 3.09 ( Fig. 1; Sakamoto et al 1995;Davis et al 1999). A rice cDNA clone, R1966, which is homologous to the barley sus gene, was mapped on rice chromosome 6 and corresponded to maize chromosome bin 9.03 and sugarcane sus QTLs (Kurata et al 1994).…”

Section: Candidate Genes For Sugar Contentmentioning

confidence: 99%

QTL Analysis in a Complex Autopolyploid: Genetic Control of Sugar Content in Sugarcane

Ming¹,

Liu²,

Moore³

et al. 2001

Genome Res.

165

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QTL mapping in autopolyploids is complicated by the possibility of segregation for three or more alleles at a locus and by a lack of preferential pairing, however the subset of polymorphic alleles that show simplex segregation ratios can be used to locate QTLs. In autopolyploid Saccharum, 36 significant associations between variation in sugar content and unlinked loci detected by 31 different probes were found in two interspecific F1 populations. Most QTL alleles showed phenotypic effects consistent with the parental phenotypes, but occasional transgressive QTLs revealed opportunities to purge unfavorable alleles from cultivars or introgress valuable alleles from exotics. Several QTLs on homologous chromosomes appeared to correspond to one another–multiple doses of favorable ‘alleles’ at such chromosomal region(s) yielded diminishing returns–such negative epistasis may contribute to phenotypic buffering. Fewer sugar content QTLs were discovered from the highest-sugar genotype than from lower-sugar genotypes, perhaps suggesting that many favorable alleles have been fixed by prior selection, i.e. that the genes for which allelic variants (QTLs) persist in improved sugarcanes may be a biased subset of the population of genes controlling sugar content. Comparison of these data to mutations and QTLs previously mapped in maize hinted that seed and biomass crops may share a partly-overlapping basis for genetic variation in carbohydrate deposition. However, many QTLs do not correspond to known candidate genes, suggesting that other approaches will be necessary to isolate the genetic determinants of high sugar content of vegetative tissues

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Structure and RFLP mapping of a rice sucrose phosphate synthase (SPS) gene that is specifically expressed in the source organ

Cited by 14 publications

References 31 publications

Analysis of cDNA Clones Encoding Sucrose-Phosphate Synthase in Relation to Sugar Interconversions Associated with Dehydration in the Resurrection Plant Craterostigma plantagineum Hochst

Analysis of cDNA Clones Encoding Sucrose-Phosphate Synthase in Relation to Sugar Interconversions Associated with Dehydration in the Resurrection Plant Craterostigma plantagineum Hochst

Protein Phosphorylation as a Mechanism for Osmotic-Stress Activation of Sucrose-Phosphate Synthase in Spinach Leaves

QTL Analysis in a Complex Autopolyploid: Genetic Control of Sugar Content in Sugarcane

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