Structure and properties of the exopolysaccharides produced by Pseudomonas mutabilis T6 and P. mutabilis ATCC 31014

Kuźma, Monika; Clack, Beatrice; Edwards, Jennifer; Tylingo, Robert; Samaszko, Justyna; Madaj, Janusz

doi:10.1016/j.carres.2011.11.012

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…Therefore, they are mainly investigated for their activity, whereas the deep structural investigation is often overlooked. For example, in Pseudomonas mutabilis, the isolated mannan showed high-viscosity solution, whereas in Rhodopseudomonas palustris, the polysaccharide solutions have been reported to influence the growth of beneficial gut microbiota. Neither these studies nor those reporting the structure and the activities of mannans isolated from fungi have considered the structure/activity relationships.…”

Section: Discussionmentioning

confidence: 99%

Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides

et al. 2021

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Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae . Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS, respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation.

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Section: Discussionmentioning

confidence: 99%

Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides

et al. 2021

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“…P. mutabilis T6 showed a 30 times higher viscosity in comparison with P. mutabilis ATCC 31014. While the exopolymers of the two strains were largely identical, consisting of D‐mannose connected by α‐(1–6) linkages, only the EPS of P. mutabilis T6 comprised 5% residual ß‐D‐glucose (Kuzma et al ., ). Other studies on a range of marine and freshwater bacteria again associated functional groups such as acetyl and pyruvate ketal groups with physical properties such as binding of metals; both are simultaneously enhanced during the stationary phase (Ford et al ., ; Sutherland, ).…”

Section: The Composition and Structural Elucidation Of Eps Componentsmentioning

confidence: 97%

“…The study of Kuzma et al . () is an exception where EPS structure and rheological properties of two different Pseudomonas strains were addressed by NMR and ESI‐MS. P. mutabilis T6 showed a 30 times higher viscosity in comparison with P. mutabilis ATCC 31014.…”

Section: The Composition and Structural Elucidation Of Eps Componentsmentioning

confidence: 99%

Biostabilization of cohesive sediments: revisiting the role of abiotic conditions, physiology and diversity of microbes, polymeric secretion, and biofilm architecture

2014

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In aquatic habitats, micro-organisms successfully adhere to and mediate particles, thus changing the erosive response of fine sediments to hydrodynamic forcing by secreting glue-like extracellular polymeric substances (EPS). Because sediment dynamics is vital for many ecological and economic aspects of watersheds and coastal regions, biostabilization of cohesive sediments is one of the important ecosystem services provided by biofilms. Although the research on biostabilization has gained momentum over the last 20 years, we still have limited insights principally due to the complex nature of this topic, the varying spatial, temporal, and community scales examined, oversimplified ecohydraulic experiments with little natural relevance, and the often partial views of the disciplines involved. This review highlights the current state of our knowledge on biostabilization and identifies important areas for future research on: (A) the influence of abiotic conditions on initial colonization and subsequent biofilm growth, focusing on hydrodynamics, substratum, salinity, nutrition, and light climate; (B) the response of microbes in terms of physiological activity and species diversity to environmental settings as well as biotic conditions such as competition and grazing; and (C) the effects of the former on the EPS matrix, its main constituents, their composition, functional groups/substitutes, and structures/linkages. The review focuses specifically on how the numerous mutual feedback mechanisms between abiotic and biotic conditions influence microbial stabilization capacity, and thus cohesive sediment dynamics.

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“…The lower molecular weight mannan was associated with higher antioxidant activity than the longer mannan and could be used as possible food supplement or ingredient in the pharmaceutical industry [55]. Recently, about 20-fold increase in mannan production has been reported in the pathogenic, constitutive biotin-producing Pseudomonas mutabilis bacteria [56]. The rheological properties of the highly branched mannan isolated from P. mutabilis T6 showed that its viscosity was over 30 times greater than that of the wild type P. mutabilis ATCC 31014.…”

Section: Sources Of Mannansmentioning

confidence: 99%

Mannan as a Promising Bioactive Material for Drug Nanocarrier Systems

Yıldız¹,

Öner²

2014

Application of Nanotechnology in Drug Delivery

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Structure and properties of the exopolysaccharides produced by Pseudomonas mutabilis T6 and P. mutabilis ATCC 31014

Cited by 10 publications

References 22 publications

Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides

Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides

Biostabilization of cohesive sediments: revisiting the role of abiotic conditions, physiology and diversity of microbes, polymeric secretion, and biofilm architecture

Mannan as a Promising Bioactive Material for Drug Nanocarrier Systems

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