Structure and properties of the potato chymotrypsin inhibitor

Valueva, T. A.; Parfenov, I. A.; Revina, T. A.; Морозкина, Е. В.; Benevolensky, Sergeii V.

doi:10.1016/j.plaphy.2011.10.021

Cited by 8 publications

(4 citation statements)

References 20 publications

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“…8 ) is strongly supported by the prevention of dependence receptor loss by a Bowman-Birk inhibitor. These compounds are selective inhibitors of serine proteases [ 152 ] and are produced by many species of plant including soybeans, lentils [ 48 ], wheat [ 153 ], potatoes [ 49 , 154 ] and other sources [ 50 , 155 ]. They are highly resistant to heat and metabolism, passing largely unchanged through the gut after dietary consumption.…”

Section: Discussionmentioning

confidence: 99%

Selective depletion of tumour suppressors Deleted in Colorectal Cancer (DCC) and neogenin by environmental and endogenous serine proteases: linking diet and cancer

et al. 2016

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BackgroundThe related tumour suppressor proteins Deleted in Colorectal Cancer (DCC) and neogenin are absent or weakly expressed in many cancers, whereas their insertion into cells suppresses oncogenic behaviour. Serine proteases influence the initiation and progression of cancers although the mechanisms are unknown.MethodsThe effects of environmental (bacterial subtilisin) and endogenous mammalian (chymotrypsin) serine proteases were examined on protein expression in fresh, normal tissue and human neuroblastoma and mammary adenocarcinoma lines. Cell proliferation and migration assays (chemoattraction and wound closure) were used to examine cell function. Cells lacking DCC were transfected with an ectopic dcc plasmid.ResultsSubtilisin and chymotrypsin selectively depleted DCC and neogenin from cells at nanomolar concentrations without affecting related proteins. Cells showed reduced adherence and increased migration, but after washing they re-attached within 24 h, with recovery of protein expression. These effects are induced by chymotryptic activity as they are prevented by chymostatin and the soybean Bowman-Birk inhibitor typical of many plant protease inhibitors.Conclusions Bacillus subtilis, which secretes subtilisin is widely present in soil, the environment and the intestinal contents, while subtilisin itself is used in meat processing, animal feed probiotics and many household cleaning agents. With chymotrypsin present in chyme, blood and tissues, these proteases may contribute to cancer development by depleting DCC and neogenin. Blocking their activity by Bowman-Birk inhibitors may explain the protective effects of a plant diet. Our findings identify a potential non-genetic contribution to cancer cell behaviour which may explain both the association of processed meats and other factors with cancer incidence and the protection afforded by plant-rich diets, with significant implications for cancer prevention.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-016-2795-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Selective depletion of tumour suppressors Deleted in Colorectal Cancer (DCC) and neogenin by environmental and endogenous serine proteases: linking diet and cancer

et al. 2016

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“…The screening of 17 cultivars was firstly carried out using the dot blot technique, which provided a direct method for screening trypsin inhibitor protein in crude extracts of various cultivars, whereas the proteinase inhibition assay resulted in a quantitative amount of trypsin inhibitor in these cultivars. H. armigera infestation studies on the fields provided a connection between pest resistance and the presence of trypsin inhibitor, as studies confirmed the idea that inhibitors of serine proteinase interfere with the digestive processes of insects (Rai et al 2008, Valueva et al 2012.…”

Section: Purification and Biochemical Activity Of Catimentioning

confidence: 87%

Purification of trypsin inhibitor from seeds of Cicer arietinum (L.) and its insecticidal potential against Helicoverpa armigera (Hübner)

Meera¹,

Sandhu²,

Babbar³

2013

Theor. Exp. Plant Physiol.

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“…The crude extraction and chromatographic separation were principally carried out according to the study by Pouvreau and Valueva. , Potatoes were mixed with MQ water, followed by the precipitation of ammonium sulfate. After heat-shocking of the resuspension at 56 °C for 15 min, the supernatant was reprecipitated by ammonium sulfate, and the resuspension was applied to desalting, cation exchange, and size-exclusion chromatography.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Serine Protease Inhibitor from Solanum tuberosum Conjugated to Soluble Dextran and Particle Carriers

2019

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A serine protease inhibitor was extracted from potato tubers. The inhibitor was conjugated to soluble, prefractionated dextran and titanium dioxide and zinc oxide nanoparticles. Conjugation to dextran was achieved by periodate oxidation of the dextran, followed by Schiff base coupling to inhibitor amino groups, and finally reduction, whereas the conjugation to the oxide particles was carried out by aminosilanization and carbonyldiimidazole activation. The inhibitory effect of the conjugated inhibitor was compared to that of free inhibitor in solution and with gelatin gel as a direct substrate. A certain degree of inhibitory activity was retained for both the dextran-conjugated and particle-conjugated inhibitors. In particular, the apparent Ki value of the dextran-conjugated inhibitor was found to be in the same range as that for free inhibitor. The dextran conjugate retained a higher activity than the free inhibitor after 1 month of storage at room temperature.

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Structure and properties of the potato chymotrypsin inhibitor

Cited by 8 publications

References 20 publications

Selective depletion of tumour suppressors Deleted in Colorectal Cancer (DCC) and neogenin by environmental and endogenous serine proteases: linking diet and cancer

Selective depletion of tumour suppressors Deleted in Colorectal Cancer (DCC) and neogenin by environmental and endogenous serine proteases: linking diet and cancer

Purification of trypsin inhibitor from seeds of Cicer arietinum (L.) and its insecticidal potential against Helicoverpa armigera (Hübner)

Characterization of Serine Protease Inhibitor from Solanum tuberosum Conjugated to Soluble Dextran and Particle Carriers

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