Structure and<i>in vitro</i>hypoglycemic activity of a homogenous polysaccharide purified from<i>Sargassum pallidum</i>

Li, Chao; Zhang, Bin; Li, Chao; Huang, Qiang; Fu, Xiong; Liu, Rui Hai

doi:10.1039/c8fo02525h

Cited by 41 publications

(21 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Induction of IR‐HepG2 model was based on previous researches (Cao, Li, et al., 2019; Cao, Zhang, et al., 2019). HepG2 cells were seeded in a 96‐well plate at 1 × 10 5 cells/well and cultured for 24 hr.…”

Section: Methodsmentioning

confidence: 99%

Hypoglycemic effect of Taraxacum officinale root extract and its synergism with Radix Astragali extract

Chai

Guo

et al. 2021

Food Science & Nutrition

View full text Add to dashboard Cite

Taraxacum officinale (dandelion) and Radix Astragali are traditional medicinal and edible plants with high nutritional value. In this study, the synergistic hypoglycemic effect of DRE and Radix Astragali extract (RAE) was evaluated. Our results showed that water extract of dandelion (DRE‐w), mainly containing polysaccharides (63.92 ± 1.82 mg/g), total flavonoid (2.57 ± 0.06 mg/g), total phenolic compounds (8.93 ± 0.34 mg/g), and saponins (0.54 ± 0.05 mg/g), exhibited significantly inhibitory effect on α‐glucosidase and α‐amylase. DRE‐w and RAE had synergistic hypoglycemic effect; we found that DRE‐w and its combination with RAE could relieve the state of insulin resistance in IR‐HepG2 cells. The combination could more significantly increase the glucose consumption and intracellular glycogen content, and improve the activity of hexokinase and pyruvate kinase in IR‐HepG2 cells. In summary, DRE and its combination with RAE can be developed as the drugs or functional foods for diabetes prevention and treatment.

show abstract

Section: Methodsmentioning

confidence: 99%

Hypoglycemic effect of Taraxacum officinale root extract and its synergism with Radix Astragali extract

Chai

Guo

et al. 2021

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…The extraction process of S. pallidum polysaccharides (SPP) was performed according to the previously reported method (Cao et al, 2019). Briefly, the sample was added to 95% (v/v) ethanol solution with a solid/ liquid ratio of 1:8 g/mL, and was heated at 70°C for 3 h in order to remove small molecules such as lipid, oligosaccharides, polyphenols and pigment.…”

Section: Preparation Of Polysaccharides By Fractional Ethanol Precipitationmentioning

confidence: 99%

“…The molecular weights of the samples were measured by high performance gel permeation chromatography (HPGPC) according to previous method (Cao et al, 2019). TSK-GEL columns (Tosoh Co., Ltd, Tokyo, Japan) coupled with an Agilent instrument equipped with Agilent 1260 refractive index detector (RID) were used.…”

Section: Determination Of Molecular Weightmentioning

confidence: 99%

“…Sargassum pallidum (Turn.) C. Agardh, which belongs to the family of Sargassum, is a typical brown alga widely distributed in China and other Asian countries (Li et al, 2017a;Cao et al, 2019). This alga is an edible and medicinal resource for treating many chronic diseases (Yuan et al, 2020a).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have reported that S. pallidum contains abundant bioactive compounds, such as polysaccharides, polyphenols, flavonoids, essential amino acids, triterpenoids and etc. In particular, S. pallidum polysaccharides have been found to exert antioxidant, hypoglycemic, prebiotic and antitumour activities (Cao et al, 2018;Cao et al, 2019;Yuan et al, 2020b).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Physicochemical properties and bioactivity of polysaccharides from Sargassum pallidum by fractional ethanol precipitation

Zhang

Yuan

et al. 2021

Int J of Food Sci Tech

Self Cite

View full text Add to dashboard Cite

Three polysaccharides (SPP-30, SPP-60 and SPP-90) were fractionated from Sargassum pallidum by 30%, 60% and 90% (v/v) ethanol, successively. Their physicochemical properties, antioxidant and antibacterial activities of SPP-30, SPP-60 and SPP-90 were comparatively investigated. The results showed that three polysaccharide fractions contained different neutral sugar, protein, uronic acid and sulphate contents. SPP-30 had the highest molecular weight (3.77-206.73 kDa), followed by SPP-60 (5.55-189.44 kDa) and SPP-90 (4.92 kDa). SPP-30, SPP-60 and SPP-90 were composed of fucose, arabinose, galactose, glucose, xylose and mannose but with different molar ratios. Scanning electron microscopy analysis suggested that they had different surface morphology and sizes of fragmentation. In vitro antioxidant assays suggested that SPP-30 and SPP-90 exhibited stronger scavenging activities against DPPH, ABTS and hydroxyl radicals than SPP-60. Moreover, SPP-30 and SPP-60 exhibited antibacterial activities against Staphylococcus aureus, while SPP-90 exhibited moderate antibacterial activity against both Staphylococcus aureus and Escherichia coli.

show abstract

Fucoidan derived from Sargassum pallidum alleviates metabolism disorders associated with improvement of cardiac injury and oxidative stress in diabetic mice

Lin

Wang

Yan

et al. 2023

Phytotherapy Research

View full text Add to dashboard Cite

Type 2 diabetes mellitus (T2DM) and its complications have become a serious global health epidemic. Cardiovascular complications have considered as a major cause of high mortality in diabetic patients. Fucoidans from brown algae have diverse medicinal activities, however, few studies reported pharmacological activity of Sargassum. pallidum fucoidan (Sp‐Fuc). Therefore, the aim of this study was to investigate the effects of Sp‐Fuc on diabetic symptoms and cardiac injury in spontaneous diabetic db/db mice. SP‐Fuc at 200 mg/(kg/d) was administered intragastrically to db/db mice for 8 weeks, the effects on hyperlipidemia, hyperglycemia, insulin resistance, and cardiac damage, as well as oxidative stress, inflammation, Nrf2/ARE, and NF‐κB signaling pathways, were investigated. Our data demonstrated that Sp‐Fuc significantly (p < 0.05) decreased body weights, hyperlipidemia, and hyperglycemia in db/db mice, along with improved insulin sensitivity. Additionally, Sp‐Fuc significantly (p < 0.05) alleviated cardiac dysfunction and pathological morphology of cardiac tissue. Sp‐Fuc also significantly (p < 0.05) decreased lipid peroxidation, increased antioxidant function, as well as reduced cardiac inflammation, possibly through Nrf2/ARE and NF‐κB signaling. Sp‐Fuc can ameliorate the metabolism disorders of glucose and lipid in diabetic mice by activating Nrf2/ARE antioxidant signaling, simultaneously reducing cardiac redox imbalance and inflammatory damage. The present findings provide a perspective on the therapy strategy for T2DM and its complications.

show abstract

Structure andin vitrohypoglycemic activity of a homogenous polysaccharide purified fromSargassum pallidum

Abstract: A homogeneous polysaccharide (PSP-2) from Sargassum pallidum and its hypoglycemic activity.

Cited by 41 publications

References 38 publications

Hypoglycemic effect of Taraxacum officinale root extract and its synergism with Radix Astragali extract

Hypoglycemic effect of Taraxacum officinale root extract and its synergism with Radix Astragali extract

Physicochemical properties and bioactivity of polysaccharides from Sargassum pallidum by fractional ethanol precipitation

Fucoidan derived from Sargassum pallidum alleviates metabolism disorders associated with improvement of cardiac injury and oxidative stress in diabetic mice

Contact Info

Product

Resources

About