Structure and Functional Expression of a Member of the Low Voltage-Activated Calcium Channel Family

Soong, Tuck Wah; Stea, Anthony; Hodson, Connie D.; Dübel, Stefan; Vincent, Steven R.; Snutch, Terry P.

doi:10.1126/science.8388125

Cited by 542 publications

(439 citation statements)

References 41 publications

Supporting

Mentioning

402

Contrasting

Unclassified

Order By: Relevance

“…The only cloned member of the low-threshold Ca 2+ channels is the rat brain ctlE subunit [34]. This channel shows transient kinetics when expressed in Xenopus oocytes, and is sensitive to external Ni 2+ (ICs0 = 28 gM).…”

Section: Resultsmentioning

confidence: 99%

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

Liévano¹,

Santi

Serrano³

et al. 1996

FEBS Letters

160

153

View full text Add to dashboard Cite

There is pharmacological evidence that Ca 2+ channels play an essential role in triggering the mammalian sperm acrosome reaction, an exocytotic process required for sperm to fertilize the egg. Spermatozoa are small terminally differentiated cells that are difficult to study by conventional electrophysiological techniques. To identify the members of the voltagedependent Ca z+ channel family possibly present in sperm, we have looked for the expression of the a~A, tXlB, ale, atD and a~E genes in mouse testis and in purified spermatogenic cell populations with RT-PCR. Our results indicate that all 5 genes are expressed in mouse testis, and in contrast only alE, and to a minor extent CqA, are expressed in spermatogenic cells. In agreement with these findings, only T-type Ca 2+ channels sensitive to the dihydropyridine nifedipine were observed in patch-clamp recordings of pachytene s permatocytes. These 2+ results suggest that low-threshold Ca channels are the dihydropyridine-sensitive channels involved in the sperm acrosome reaction.

show abstract

Section: Resultsmentioning

confidence: 99%

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

Liévano¹,

Santi

Serrano³

et al. 1996

FEBS Letters

160

153

View full text Add to dashboard Cite

show abstract

“…This class contains several types of calcium channels: N-type, 12,16,42,45 Q-type 39 or R-type. 11,38 To what extent each of the candidates is present could not be determined on the basis of voltage-dependent and kinetic properties alone.…”

Section: Current Separationmentioning

confidence: 99%

Calcium currents in pyramidal CA1 neurons in vitro after kindling epileptogenesis in the hippocampus of the rat

1996

View full text Add to dashboard Cite

Calcium currents in pyramidal CA1 neurons in vitro after kindling epileptogenesis in the hippocampus of the rat Faas, G.C.; Vreugdenhil, M.; Wadman, W.J. Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Abstract--Calcium is an important second messenger which plays a role in the regulation of neuronal excitability and in many forms of synaptic plasticity. In kindling epileptogenesis, a model of focal epilepsy, calcium plays an important role. The in situ patch-clamp technique was used to record calcium currents in slices obtained from kindled rats and controls. We found that low-voltage-activated calcium currents, probably of dendritic origin, were larger after kindling (80%). The transient high-voltage-activated calcium currents were also enhanced after kindling (50% higher). The increase of the current is accompanied by a decrease in the time constant of inactivation. The change was still present six weeks after the kindling stimulations were stopped. These data demonstrate that low-voltage-activated calcium currents are involved in epileptogenesis. Their enhancement in the dendrites will boost synaptic depolarization and result in enhanced calcium influx, which is critically dependent on the specific activation pattern.

show abstract

“…Both domains determine the attachment of the fl subunit to the c~ 1 channel. This linkage is required for the observed 13 subunit regulation of the current properties of the C~l subunit, that is the increase in current amplitude [6][7][8][9][10] and the modifications in kinetics and voltage dependence of the channel [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Identification of critical amino acids involved in α₁‐β interaction in voltage‐dependent Ca²⁺ channels

et al. 1996

View full text Add to dashboard Cite

In voltage-dependent Ca 2+ channels, the al and subunits interact via two cytoplasmic regions defined as the Alpha Interaction Domain (AID) and Beta Interaction Domain (BID). Several novel amino acids for that interaction have now been mapped in both domains by point mutations. It was found that three of the nine amino acids in AID and four of the eight BID amino acids tested were essential for the interaction. Whereas the important AID amino acids were clustered around five residues, the important BID residues were more widely distributed within a larger 16 amino acid sequence. The affinity of the AID A GST fusion protein for the four interacting/]lb BID mutants was not significantly altered compared with the wild-type ~b despite the close localization of mutated residues to disruptive BID amino acids. Expression of these interactive S] mutants with the fulllength OLIA subunit only slightly modified the stimulation efficiency when compared with the wild-type ~b subunit. Our data suggest that non-disruptive BID sequence alterations do not dramatically affect the/] subunit-induced current stimulation.

show abstract

Structure and Functional Expression of a Member of the Low Voltage-Activated Calcium Channel Family

Cited by 542 publications

References 41 publications

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

Calcium currents in pyramidal CA1 neurons in vitro after kindling epileptogenesis in the hippocampus of the rat

Identification of critical amino acids involved in α₁‐β interaction in voltage‐dependent Ca²⁺ channels

Contact Info

Product

Resources

About

Structure and Functional Expression of a Member of the Low Voltage-Activated Calcium Channel Family

Cited by 542 publications

References 41 publications

T‐type Ca2+ channels and α1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

T‐type Ca2+ channels and α1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

Calcium currents in pyramidal CA1 neurons in vitro after kindling epileptogenesis in the hippocampus of the rat

Identification of critical amino acids involved in α1‐β interaction in voltage‐dependent Ca2+ channels

Contact Info

Product

Resources

About

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

Identification of critical amino acids involved in α₁‐β interaction in voltage‐dependent Ca²⁺ channels