Structure and function of SWI/SNF chromatin remodeling complexes and mechanistic implications for transcription

Tang, Liling; Nogales, Eva; Ciferri, Claudio

doi:10.1016/j.pbiomolbio.2010.05.001

Cited by 192 publications

(165 citation statements)

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“…The SWI/SNF and ISWI complexes regulate gene expression through distinct molecular mechanisms (Kadam and Emerson, 2003;Narlikar et al, 2002;Tang et al, 2010). Nevertheless, similar defects in nuclear degradation were observed in both Brg1 (He et al, 2010) and Snf2h (Figs 2 and 5) cKO lenses.…”

Section: Molecular Analysis Of the Lens Fiber Cell Denucleation Pathwmentioning

confidence: 61%

Chromatin remodeling enzyme Snf2h regulates embryonic lens differentiation and denucleation

Limi

McGreal

et al. 2016

Development

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Ocular lens morphogenesis is a model for investigating mechanisms of cellular differentiation, spatial and temporal gene expression control, and chromatin regulation. Brg1 (Smarca4) and Snf2h (Smarca5) are catalytic subunits of distinct ATP-dependent chromatin remodeling complexes implicated in transcriptional regulation. Previous studies have shown that Brg1 regulates both lens fiber cell differentiation and organized degradation of their nuclei (denucleation). Here, we employed a conditional Snf2h flox mouse model to probe the cellular and molecular mechanisms of lens formation. Depletion of Snf2h induces premature and expanded differentiation of lens precursor cells forming the lens vesicle, implicating Snf2h as a key regulator of lens vesicle polarity through spatial control of Prox1, Jag1, p27Kip1 (Cdkn1b) and p57Kip2 (Cdkn1c) gene expression. The abnormal Snf2h −/− fiber cells also retain their nuclei. RNA profiling of Snf2h −/− and Brg1 −/− eyes revealed differences in multiple transcripts, including prominent downregulation of those encoding Hsf4 and DNase IIβ, which are implicated in the denucleation process. In summary, our data suggest that Snf2h is essential for the establishment of lens vesicle polarity, partitioning of prospective lens epithelial and fiber cell compartments, lens fiber cell differentiation, and lens fiber cell nuclear degradation.

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Section: Molecular Analysis Of the Lens Fiber Cell Denucleation Pathwmentioning

confidence: 61%

Chromatin remodeling enzyme Snf2h regulates embryonic lens differentiation and denucleation

Limi

McGreal

et al. 2016

Development

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“…Close counterparts of RSC have been identified in humans and other eukaryotes. Structural and mechanistic studies have borne out the close relationship between yeast RSC and SWI/SNF complexes and the corresponding human complexes (Tang et al 2010).…”

Section: Chromatin-remodelingmentioning

confidence: 99%

Chromatin-remodeling and the initiation of transcription

Lorch

Kornberg

2015

Quart. Rev. Biophys.

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“…These include the HAT enzymes PCAF and GCN5, the Tip60 acetyltransferase, the co-activator CBP, the transcriptional protein TAF1, BRD2, BPTF, SNF2L4, BAZ2A, BAZ2B, and the nuclear proteins Sp100, Sp110 and Sp140. [119][120][121] Moreover, many proteins that contain BRDs also have other domains including PHD, PWWP, B-box type zinc finger, Ring finger, SAND, FY Rich, SET, TAZ zinc finger, helicase, ATPase, BAH, WD40 repeat and MBDs. 119 The complexity of BRDcontaining proteins role in the regulation of gene expression can be exemplified by the BRDs in human SWI/SNF BRG1 and hBRM ATPases, which have dynamic capacity to bind H3K14ac, H4K8ac, H4K5ac and H4K122ac, as well as DNA, via AT-hook or zinc finger binding motifs.…”

Section: Heterochromatin Proteinsmentioning

confidence: 99%