Abstract:We have examined in vitro the morphology and visual response properties of retinal ganglion cells innervating a component of the cat's lateral geniculate nucleus known as the geniculate wing (or retinorecipient zone of the pulvinar). Ganglion cells were first labeled in situ by retrograde transport of fluorescent microspheres from the geniculate wing. Labeled cells were injected intracellular with Lucifer yellow and biocytin in the isolated retina and visualized immunohistochemically. With one exception, stain… Show more
“…These 3 morphological types of ganglion cells, α-, β-, and γ-cells corresponded to the physiologically defined groups of Y-, X-, and W-cells, respectively [4,15,38]. So far, RGCs have been divided into 3 major types in most mammals, but it is probable that they could be classified more precisely [25,29,36].…”
Section: Intracellular Filling With Lucifer Yellow (Ly)mentioning
ABSTRACT. Classification of retinal ganglion cells (RGCs) in the chick central retina was studied by retrograde labeling of carbocyanine dye (DiI) and intracellular filling with Lucifer Yellow. Ganglion cells were divided into 4 groups, Group Ic/Is, Group IIc/IIs, Group IIIs, Group IVc, according to sizes of somal area and dendritic field and dendritic branching pattern. Group I cells had small somal area and small dendritic field. They were further divided into 2 subgroups by complexity (subgroup Ic) and simplicity (subgroup Is) of the dendritic arborization. Group II cells had medium-sized soma and dendritic field. They were also divided into subgroup IIc and IIs by the same definitions as those of subgroup Ic and Is. Group IIIs had medium-sized soma, large and simple dendritic arborization. Group IVc in which all cells had large soma, showed large and complex dendritic arborization. Cell populations of each group were 51.8% (subgroup Ic), 21.1% (subgroup Is), 6.2% (subgroup IIc), 14.6% (subgroup IIs), 4.2% (Group IIIs), and 2.1% (Group IVc). Subgroup Ic cells, which were very similar to β-cells in the mammalian central area, represented about a half of the ganglion cell population. Cells in subgroup Is and IIs, which were not reported in the mammalian retina, were found in the chick central retina in relatively high population (35.7%). Morphological features of chick RGCs in the central retina were considered in comparison with those of other vertebrates.-KEY WORDS: bird, cell type, ganglion cell, intracellular filling, retina.
“…These 3 morphological types of ganglion cells, α-, β-, and γ-cells corresponded to the physiologically defined groups of Y-, X-, and W-cells, respectively [4,15,38]. So far, RGCs have been divided into 3 major types in most mammals, but it is probable that they could be classified more precisely [25,29,36].…”
Section: Intracellular Filling With Lucifer Yellow (Ly)mentioning
ABSTRACT. Classification of retinal ganglion cells (RGCs) in the chick central retina was studied by retrograde labeling of carbocyanine dye (DiI) and intracellular filling with Lucifer Yellow. Ganglion cells were divided into 4 groups, Group Ic/Is, Group IIc/IIs, Group IIIs, Group IVc, according to sizes of somal area and dendritic field and dendritic branching pattern. Group I cells had small somal area and small dendritic field. They were further divided into 2 subgroups by complexity (subgroup Ic) and simplicity (subgroup Is) of the dendritic arborization. Group II cells had medium-sized soma and dendritic field. They were also divided into subgroup IIc and IIs by the same definitions as those of subgroup Ic and Is. Group IIIs had medium-sized soma, large and simple dendritic arborization. Group IVc in which all cells had large soma, showed large and complex dendritic arborization. Cell populations of each group were 51.8% (subgroup Ic), 21.1% (subgroup Is), 6.2% (subgroup IIc), 14.6% (subgroup IIs), 4.2% (Group IIIs), and 2.1% (Group IVc). Subgroup Ic cells, which were very similar to β-cells in the mammalian central area, represented about a half of the ganglion cell population. Cells in subgroup Is and IIs, which were not reported in the mammalian retina, were found in the chick central retina in relatively high population (35.7%). Morphological features of chick RGCs in the central retina were considered in comparison with those of other vertebrates.-KEY WORDS: bird, cell type, ganglion cell, intracellular filling, retina.
“…Several other anatomical types have coverage factors greater than two (Rodieck 1998;Wässle and Boycott 1991), and even as large as six (Berson 2003;Pu et al 1994). In a recent anatomical classification of rabbit ganglion cells, Rockhill et al (2002) found that their 13 morphological types needed to have an average coverage factor of 3.2 to account for the ganglion cell density that they measured.…”
Segev, Ronen, Jason Puchalla, and Michael J. Berry II. Functional organization of ganglion cells in the salamander retina. J Neurophysiol 95: 2277-2292, 2006. First published November 23, 2005 doi:10.1152/jn.00928.2005. Recently, we reported a novel technique for recording all of the ganglion cells in a retinal patch and showed that their receptive fields cover visual space roughly 60 times over in the tiger salamander. Here, we carry this analysis further and divide the population of ganglion cells into functional classes using quantitative clustering algorithms that combine several response characteristics. Using only the receptive field to classify ganglion cells revealed six cell types, in agreement with anatomical studies. Adding other response measures served to blur the distinctions between these cell types rather than resolve further classes. Only the biphasic OFF type had receptive fields that tiled the retina. Even when we attempted to split these classes more finely, ganglion cells with almost identical functional properties were found to have strongly overlapping spatial receptive fields. A territorial spatial organization, where ganglion cell receptive fields tend to avoid those of other cells of the same type, was only found for the biphasic OFF cell. We further studied the functional segregation of the ganglion cell population by computing the amount of visual information shared between pairs of cells under natural movie stimulation. This analysis revealed an extensive mixing of visual information among cells of different functional type. Together, our results indicate that the salamander retina uses a population code in which every point in visual space is represented by multiple neurons with subtly different visual sensitivities.
I N T R O D U C T I O NIn now classic work, Wässle and colleagues showed that the alpha ganglion cell in the cat, which is distinguished by its large soma size, formed two plexuses with their dendritic arbors-one with ON-type light responses and the other with OFF-type-that precisely covered visual space (Wässle and Boycott 1991). The somas of neighboring cells tended to be spaced one dendritic diameter apart from one another, the tips of their dendrites just barely touching. A similar territorial organization and dendritic coverage factors close to one have been found in other, prominent morphological types of ganglion cells both in the monkey and the rabbit (Dacey 1993;Vaney 1994). Tight correspondences between anatomy and function have also been found (Wässle and Boycott 1991), along with an increasingly intricate functional segregation of axons and dendrites in the inner plexiform layer (MacNeil et al. 1999;Pang et al. 2002;Roska and Werblin 2001;Sterling 1983;Wassle 2004;Zhang et al. 2004). For all these reasons, tiling has come to be regarded as a fundamental principle of retinal organization.However, this simple picture of efficient tiling does not hold for all of the ganglion cells. Several other anatomical types have coverage factors greater than two (Rodieck ...
“…Subsets of mammalian ganglion cell classes project to an array of central targets (Fukuda and Stone, 1974;Farmer and Rodieck, 1982;Leventhal et al, 1985;Rodieck and Watanabe, 1993;Pu et al, 1994;Rodieck, 1998), but a unified description of all classes and distributions in the ganglion cell layer has remained elusive. Several summaries of how neuronal typologies might be abstracted have emerged, some accompanied by debates regarding methods, definitions, and results (Rowe and Stone, 1977;Hughes, 1979;Holden, 1981;Rodieck and Brening, 1982;Famiglietti, 1992;Wingate et al, 1992;Cook, 1998;Masland and Raviola, 2000).…”
Classifying all of the ganglion cells in the mammalian retina has long been a goal of anatomists, physiologists, and cell biologists. The rabbit retinal ganglion cell layer was phenotyped using intrinsic small molecule signals (aspartate, glutamate, glycine, glutamine, GABA, and taurine) and glutamate receptorgated 1-amino-4-guanidobutane excitation signals as the clustering dimensions for formal classification. Intrinsic signals alone yielded 7 ganglion cell superclasses and 1 amacrine cell superclass; the addition of excitation signals ultimately resolved 14 natural ganglion cell classes and 3 amacrine cell classes. Ganglion cells comprise two-thirds to three-quarters of the cells in the ganglion cell layer and exhibited distinct metabolic, coupling, and excitation phenotypes, as well as characteristic sizes, population fractions, and patterns. Metabolic signatures (mixtures of glutamate, aspartate, glutamine, and GABA) chemically discriminated ganglion from amacrine cells. Coupling signatures reflected heterologous coupling states across ganglion cells: (1) uncoupled, (2) coupled to GABAergic amacrine cells, and (3) coupled to glycinergic amacrine cells. Excitation signatures reflected differential channel permeation rates across classes after AMPA activation. Extraction of unique size and patterning features from the data sets further validated the robustness of the classification. Because the classifications were explicitly blinded to structure, this is strong evidence that molecular phenotype classes are natural classes. Correspondences of molecular phenotype classes to functional classes were inferred from size, coupling, encounter, and physiological attributes. Ganglion cell classes display markedly different ionotropic drives, which may partly explain the physiological brisk-sluggish spectrum of ganglion cell spiking patterns.
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