Structure and Function of Human Matrix Metalloproteinases

Laronha, Helena; Caldeira, Jorge

doi:10.3390/cells9051076

Cited by 349 publications

(397 citation statements)

References 35 publications

Supporting

Mentioning

331

Contrasting

Unclassified

Order By: Relevance

“…As shown in Figure 2 , the spherical catalytic domains share the same structural organization: three α-helixes, five β-sheets, connected by eight loops. Additionally, they contain a catalytic zinc ion coordinated by three histidine residues, a structural zinc ion, and three structural calcium ions required for enzyme stability [ 37 ]. The specificity of the MMP-substrate interaction depends on specific subsites or pockets (S) within the MMP molecule that interacts with corresponding substituents (P) in the substrate.…”

Section: Basic Aspects Of Mmp-13mentioning

confidence: 99%

Overview of MMP-13 as a Promising Target for the Treatment of Osteoarthritis

Ecker

2021

IJMS

195

134

View full text Add to dashboard Cite

Osteoarthritis (OA) is a common degenerative disease characterized by the destruction of articular cartilage and chronic inflammation of surrounding tissues. Matrix metalloproteinase-13 (MMP-13) is the primary MMP involved in cartilage degradation through its particular ability to cleave type II collagen. Hence, it is an attractive target for the treatment of OA. However, the detailed molecular mechanisms of OA initiation and progression remain elusive, and, currently, there are no interventions available to restore degraded cartilage. This review fully illustrates the involvement of MMP-13 in the initiation and progression of OA through the regulation of MMP-13 activity at the molecular and epigenetic levels, as well as the strategies that have been employed against MMP-13. The aim of this review is to identify MMP-13 as an attractive target for inhibitor development in the treatment of OA.

show abstract

Section: Basic Aspects Of Mmp-13mentioning

confidence: 99%

Overview of MMP-13 as a Promising Target for the Treatment of Osteoarthritis

Ecker

2021

IJMS

195

134

View full text Add to dashboard Cite

show abstract

“…MMP-9 plays a critical role in angiogenesis, immune cell migration, activation of cytokines and chemokines, and progression and metastasis of cancer cells [43][44][45] . MMP-9 degrades collagen types IV, V, XIk´, XIVl´, elastin, aggrecan, link protein, decorinr, lamininn, entactin, SPARCq, myelin basic proteinm, ∞2Mn, ∞1Pli, IL-1βj, and proTNF-∞k 46,47 . In the current study, TCs exhibited proteolytic activities that had a strong immunoaffinity for MMP-9.…”

Section: Discussionmentioning

confidence: 99%

Telocytes are major constituents of the angiogenic apparatus

Soliman

2021

Sci Rep

View full text Add to dashboard Cite

The current study investigated role of telocytes (TCs) in angiogenesis during embryonic development of quail using immunohistochemistry (IHC), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The angiogenic apparatus consisted of TCs, endothelial cells, and macrophages. TCs were identified morphologically by their telopodes and podoms using TEM and SEM and immunohistochemically using CD34, and vascular endothelial growth factor (VEGF). TCs also expressed CD68. TCs formed a three-dimensional network and established direct contact with blood vessels, sprouting endothelial cells, and active macrophages, while exerting their effect through paracrine signaling. VEGF was also expressed by endothelial cells and macrophages. Matrix metalloproteinase–9 (MMP-9) was expressed by TCs, endothelial cells, and macrophages. In conclusion, the expression of VEGF by TCs, endothelial cells, and macrophages is required for the proliferation and migration of endothelial cells and vascular growth. The expression of MMP-9 by TCs, endothelial cells, and macrophages is essential for the degradation of extracellular matrix (ECM) components during neoangiogenesis. Macrophages may facilitate phagocytosis and elimination of the degraded ECM components.

show abstract

“…The present study identified MMP13 and MMP14 as novel downstream effectors of GATA2 in trophoblast cells. MMP13 (also known as collagenase-3) is secreted into the extracellular space as an inactive proenzyme (pro-MMP13); its activation requires cleavage of pro-MMP13 by MMP3 and MMP14 [ 62 , 63 ]. Active MMP13 initiates activation of pro-MMP9 [ 64 ] and degrades native fibrillar collagen (e.g., collagen type 1/2) [ 40 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases

Ogoyama

Ohkuchi

Zhao

et al. 2021

IJMS

View full text Add to dashboard Cite

The invasion of extravillous trophoblast (EVT) cells into the maternal decidua, which plays a crucial role in the establishment of a successful pregnancy, is highly orchestrated by a complex array of regulatory mechanisms. Non-coding RNAs (ncRNAs) that fine-tune gene expression at epigenetic, transcriptional, and post-transcriptional levels are involved in the regulatory mechanisms of EVT cell invasion. However, little is known about the characteristic features of EVT-associated ncRNAs. To elucidate the gene expression profiles of both coding and non-coding transcripts (i.e., mRNAs, long non-coding RNAs (lncRNAs), and microRNAs (miRNAs)) expressed in EVT cells, we performed RNA sequencing analysis of EVT cells isolated from first-trimester placentae. RNA sequencing analysis demonstrated that the lncRNA H19 and its derived miRNA miR-675-5p were enriched in EVT cells. Although miR-675-5p acts as a placental/trophoblast growth suppressor, there is little information on the involvement of miR-675-5p in trophoblast cell invasion. Next, we evaluated a possible role of miR-675-5p in EVT cell invasion using the EVT cell lines HTR-8/SVneo and HChEpC1b; overexpression of miR-675-5p significantly promoted the invasion of both EVT cell lines. The transcription factor gene GATA2 was shown to be a target of miR-675-5p; moreover, small interfering RNA-mediated GATA2 knockdown significantly promoted cell invasion. Furthermore, we identified MMP13 and MMP14 as downstream effectors of miR-675-5p/GATA2-dependent EVT cell invasion. These findings suggest that miR-675-5p-mediated GATA2 inhibition accelerates EVT cell invasion by upregulating matrix metalloproteinases.

show abstract

Structure and Function of Human Matrix Metalloproteinases

Cited by 349 publications

References 35 publications

Overview of MMP-13 as a Promising Target for the Treatment of Osteoarthritis

Overview of MMP-13 as a Promising Target for the Treatment of Osteoarthritis

Telocytes are major constituents of the angiogenic apparatus

LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases

Contact Info

Product

Resources

About