Background: The abnormal of DHX32 was related to the occurrence, development and clinicopathological features of cancer, but DHX32 has not been studied in Lung squamous carcinoma (LUSC). We aimed to investigate the expression , prognosis and biological significance of DHX32 in LUSC.
Method: The LUSC-related datasets (GSE30219 and GSE8894) were included in the analysis. Firstly, the expression and diagnosis of DHX32 were analyzed. The differentially expressed genes (DEGs) of GSE30219 were screened. The Cox analysis was used to screen prognostic genes. Based on the risk scores of LUSC samples, the samples were divided into high-low risk groups. The risk model was constructed by cox analysis in GSE30219, and verified in GSE8894. Furthermore, nomogram, immune infiltration analysis and drug sensitivity analysis were constructed.
Results: Compared with the control group, the expression of DHX32 in LUSC samples was obviously higher. The survival of high truncation value group was signally higher than that of low truncation value group. Based on 347 DEGs, the 3 prognostic genes (CLDN11, PID1 and MAMDC2) were screened. In GSE30219 and GSE8894, the risk model had high accuracy and excellent performance. In addition, the riskScore was independent prognostic risk factors for LUSC by cox analysis. Immune infiltration analysis and drug sensitivity analysis showed that memory B cells and five drug had a difference in two risk groups.
Conclusion: DHX32 could be used as a diagnostic gene and independent prognostic factor for LUSC, providing the insight for the diagnosis and treatment of LUSC.