Structure and function of abscisic acid receptors

Miyakawa, Takuya; Fujita, Yasunari; Yamaguchi-Shinozaki, Kazuko; Tanokura, Masaru

doi:10.1016/j.tplants.2012.11.002

Cited by 168 publications

(122 citation statements)

References 82 publications

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“…No such adjustments in receptor gene expression were found in the stay-green type that maintained ABA homeostasis under long-term stress. Collectively, all these results point to different levels of ABA in short-term versus long-term stress having an impact on perception by various PYR/ PYL members that appear to have specialized functions with respect to signaling dynamic cellular ABA content (Miyakawa et al, 2013;Okamoto et al, 2013).…”

Section: Expression Dynamics Of Aba Perception Genes Under Short-termmentioning

confidence: 86%

Abscisic Acid Flux Alterations Result in Differential Abscisic Acid Signaling Responses and Impact Assimilation Efficiency in Barley under Terminal Drought Stress

et al. 2014

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Abscisic acid (ABA) is a central player in plant responses to drought stress. How variable levels of ABA under short-term versus long-term drought stress impact assimilation and growth in crops is unclear. We addressed this through comparative analysis, using two elite breeding lines of barley (Hordeum vulgare) that show senescence or stay-green phenotype under terminal drought stress and by making use of transgenic barley lines that express Arabidopsis (Arabidopsis thaliana) 9-cis-epoxycarotenoid dioxygenase (AtNCED6) coding sequence or an RNA interference (RNAi) sequence of ABA 89-hydroxylase under the control of a droughtinducible barley promoter. The high levels of ABA and its catabolites in the senescing breeding line under long-term stress were detrimental for assimilate productivity, whereas these levels were not perturbed in the stay-green type that performed better. In transgenic barley, drought-inducible AtNCED expression afforded temporal control in ABA levels such that the ABA levels rose sooner than in wild-type plants but also subsided, unlike as in the wild type , to near-basal levels upon prolonged stress treatment due to down-regulation of endogenous HvNCED genes. Suppressing of ABA catabolism with the RNA interference approach of ABA 89-hydroxylase caused ABA flux during the entire period of stress. These transgenic plants performed better than the wild type under stress to maintain a favorable instantaneous water use efficiency and better assimilation. Gene expression analysis, protein structural modeling, and protein-protein interaction analyses of the members of the PYRABACTIN RESISTANCE1/PYRABACTIN RESISTANCE1-LIKE/REGULATORY COMPONENT OF ABA RECEPTORS, TYPE 2C PROTEIN PHOSPHATASE Sucrose nonfermenting1-related protein kinase2, and ABA-INSENSITIVE5/ABA-responsive element binding factor family identified specific members that could potentially impact ABA metabolism and stress adaptation in barley.Drought compromises grain yield in cereals, especially when the stress occurs during postanthesis (Boyer and Westgate, 2004;Sreenivasulu et al., 2007).In some crops, selection for and use of stay-green types has been promoted as a means for combating drought stress susceptibility (Thomas and Howarth, 2000). However, the molecular basis of stay-green phenotype in barley (Hordeum vulgare) has not been explored in detail. As the flag leaf is the principal source organ, sustaining its photosynthetic activity under postanthesis drought stress is considered a strategy to mitigate the yield penalty. Water use efficiency (WUE) is the amount of biomass (carbon) accumulated per unit of water, hence an important trait under water-limited conditions (Condon and Richards, 1992;Rebetzke et al., 2002;Richards et al., 2002). Breeding for WUE is considered important for developing drought-tolerant crops (Blum, 1996;Richards, 1996;Richards et al., 2002). Genetic loci that control transpiration efficiency have been identified (Teulat et al., 2002;Hall et al., 2005;Juenger et al., 2005). In Arabidopsis (Arabidopsis tha...

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Section: Expression Dynamics Of Aba Perception Genes Under Short-termmentioning

confidence: 86%

Abscisic Acid Flux Alterations Result in Differential Abscisic Acid Signaling Responses and Impact Assimilation Efficiency in Barley under Terminal Drought Stress

et al. 2014

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“…However, the (Risk et al 2009;Taiz and Zeiger 2010;Miyakawa et al 2013). By contrast, the action of PYR/RCAR as an ABA receptor is well supported by several studies (Kharenko et al 2013;Gonzalez-Guzman et al 2014;Kim et al 2014).…”

Section: Induction Mechanism Under Drought Stressmentioning

confidence: 95%

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

González‐Villagra

Kurepin

2017

Planta

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Main conclusion ABA is involved in anthocyanin synthesis through the regulation of microRNA156, augmenting the level of expression of anthocyanin synthesisrelated genes and, therefore, increasing anthocyanin level.Drought stress is the main cause of agricultural crop loss in the world. However, plants have developed mechanisms that allow them to tolerate drought stress conditions. At cellular level, drought stress induces changes in metabolite accumulation, including increases in anthocyanin levels due to upregulation of the anthocyanin biosynthetic pathway. Recent studies suggest that the higher anthocyanin content observed under drought stress conditions could be a consequence of a rise in the abscisic acid (ABA) concentration. This plant hormone crosses the plasma membrane by specific transporters, and it is recognized at the cytosolic level by receptors known as pyrabactin resistance (PYR)/regulatory component of ABA receptors (PYR/RCARs) that regulate downstream components. In this review, we discuss the hypothesis regarding the involvement of ABA in the regulation of microRNA156 (miRNA156), which is upregulated as part of dehydration stress responsiveness in different species. The miRNA156 upregulation produces a greater level of anthocyanin gene expression, forming the multienzyme complex that will synthesize an increased level of anthocyanins at the cytosolic face of the rough endoplasmic reticulum (RER). After synthesis, anthocyanins are transported from the RER to the vacuole by two possible models of transport: (1) membrane vesicle-mediated transport, or (2) membrane transporter-mediated transport. Thus, the aim was to analyze the recent findings on synthesis, transport and the possible mechanism by which ABA could increase anthocyanin synthesis under drought stress conditions potentially throughout microRNA156 (miRNA156).

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“…Corroborating this analysis, mutation of the corresponding Lys to Gln in PYL2 completely abolished its affinity with ABA [11]. Ever since the discovery of PYLs being ABA receptors, extensive studies have been committed to the mechanistic elucidation of various PYL members [11,12,[14][15][16][17][18][19][20][21][22][23]; however, the function of PYL13 has remained elusive. In this report, we combine structural biology and biochemical approaches to systematically investigate the function and mechanism of PYL13.…”

Section: Introductionmentioning

confidence: 93%

Molecular basis for the selective and ABA-independent inhibition of PP2CA by PYL13

Wang

Sheng

et al. 2013

Cell Res

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PYR1/PYL/RCAR family proteins (PYLs) are well-characterized abscisic acid (ABA) receptors. Among the 14 PYL members in Arabidopsis thaliana, PYL13 is ABA irresponsive and its function has remained elusive. Here, we show that PYL13 selectively inhibits the phosphatase activity of PP2CA independent of ABA. The crystal structure of PYL13-PP2CA complex, which was determined at 2.4 Å resolution, elucidates the molecular basis for the specific recognition between PP2CA and PYL13. In addition to the canonical interactions between PYLs and PP2Cs, an extra interface is identified involving an element in the vicinity of a previously uncharacterized CCCH zinc-finger (ZF) motif in PP2CA. Sequence blast identified another 56 ZF-containing PP2Cs, all of which are from plants. The structure also reveals the molecular determinants for the ABA irresponsiveness of PYL13. Finally, biochemical analysis suggests that PYL13 may hetero-oligomerize with PYL10. These two PYLs antagonize each other in their respective ABA-independent inhibitions of PP2Cs. The biochemical and structural studies provide important insights into the function of PYL13 in the stress response of plant and set up a foundation for future biotechnological applications of PYL13.

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Structure and function of abscisic acid receptors

Cited by 168 publications

References 82 publications

Abscisic Acid Flux Alterations Result in Differential Abscisic Acid Signaling Responses and Impact Assimilation Efficiency in Barley under Terminal Drought Stress

Abscisic Acid Flux Alterations Result in Differential Abscisic Acid Signaling Responses and Impact Assimilation Efficiency in Barley under Terminal Drought Stress

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

Molecular basis for the selective and ABA-independent inhibition of PP2CA by PYL13

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