Structure and function analysis of Arabidopsis thaliana SRO protein family

Li, Baozhu; Zhao, Xiang; Zhao, Xin; Lei, Peng

doi:10.3724/sp.j.1005.2013.01189

Cited by 11 publications

(15 citation statements)

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“…Methyl jasmonate can also be used as the core signal factor of plant resistance to insect invasion [26]. Salicylic acid (SA) is an important signaling molecule for plants to cope with biotic or abiotic stress [12]. Gibberellins are phytohormones that regulate multiple developmental processes, such as seed germination, stem elongation, flowering, and fruit development [27].…”

Section: Discussionmentioning

confidence: 99%

“…Six known ID of Arabidopsis thaliana SRO genes [12] were put into Arabidopsis genome database (TAIR) [31] to obtain their protein sequences. Using Arabidopsis SRO protein sequences as probes, the candidate members of Chinese cabbage SRO family were searched and the coding sequences (CDS) and amino acid sequences of the B. rapa SRO genes were downloaded from the Brassica database [32].…”

Section: Identification and Sequence Analysis Of Sro Genes In Chinese Cabbagementioning

confidence: 99%

“…AtSRO5 interacts with transcription factors to regulate gene expression, and overexpression of AtSRO5 can increase the salt tolerance of transgenic plants by lowering the level of H 2 O 2 in the roots [11]. AtSRO2 and AtSRO3 can respond to strong light, salt, and ozone stress; AtSRO4 has no clear function reported [12]. SROs have also been partially studied in apple, rice, wheat, corn, continental cotton, tomato, and other crops.…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Analysis of SRO Gene Family in Chinese Cabbage (Brassica rapa L.)

Qiao

Gao

Liu

et al. 2020

Plants

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Similar to radical-induced cell death 1 (SROs) is a family of small proteins unique to plants. SRO transcription factors play an important role in plants’ response to biotic and abiotic stresses. In this study, we identified 12 BrSRO genes in Chinese cabbage (Brassica rapa L.). Among them, a comprehensive overview of the SRO gene family is presented, including physical and chemical characteristics, chromosome locations, phylogenetic analysis, gene structures, motif analysis, and cis-element analyses. The number of amino acids of BrSRO genes is between 77–779 aa, isoelectric point changed from 6.02 to 9.6. Of the 12 BrSRO genes, 11 were randomly distributed along the 7 chromosomes, while BrSRO12 was located along unassigned scaffolds. Phylogenetic analysis indicated that the SRO proteins from six species, including Arabidopsis, banana, rice, Solanum lycopersicum, Zea mays, and Chinese cabbage were divided into eleven groups. The exon-rich BrSRO6 and BrSRO12 containing 15 exons were clustered to group K. All 12 genes have motif 2, which indicate that motif 2 is a relatively conservative motif. There are many hormone and stress response elements in BrSRO genes. The relative expression levels of 12 BrSRO genes under high temperature, drought, salt, and low temperature conditions were analyzed by real-time fluorescence quantitative PCR. The results indicated the relative expression level of BrSRO8 was significantly up-regulated when plants were exposed to high temperature. The relative expression levels of BrSRO1, 3, 7, 8, and 9 were higher under low temperature treatment. The up-regulated genes response to drought and salt stresses were BrSRO1, 5, 9 and BrSRO1, 8, respectively. These results indicated that these genes have certain responses to different abiotic stresses. This work has provided a foundation for further functional analyses of SRO genes in Chinese cabbage.

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Section: Discussionmentioning

confidence: 99%

Section: Identification and Sequence Analysis Of Sro Genes In Chinese Cabbagementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Analysis of SRO Gene Family in Chinese Cabbage (Brassica rapa L.)

Qiao

Gao

Liu

et al. 2020

Plants

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“…Overexpression of AtSRO5 could mediate proline metabolism in Arabidopsis mitochondria, thereby improving plant salt stress and antioxidant capacity ( Borsani et al, 2005 ). AtSRO2 , AtSRO3 and AtSRO5 have shown changes in transcript levels in response to light stress, salt treatment and exposure to O 3 ( Jaspers et al, 2010b ; Li et al, 2013 ), but AtSRO4 has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Evolutionary Analysis of the SRO Gene Family in Tomato

Wang

et al. 2021

Front. Genet.

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SRO (SIMILAR TO RCD ONE) is a family of plant-specific small molecule proteins that play an important role in plant growth and development and environmental responses. However, SROs still lack systematic characterization in tomato. Based on bioinformatics methods, SRO family genes were identified and characterized from cultivated tomatoes and several wild tomatoes. qRT-PCR was used to study the expression of SRO gene in cultivated tomatoes. Phylogenetic and evolutionary analyses showed that SRO genes in angiosperms share a common ancestor and that the number of SRO family members changed as plants diverged and evolved. Cultivated tomato had six SRO members, five of which still shared some degree of identity with the ancestral SRO genes. Genetic structure and physicochemical properties showed that tomato SRO genes were highly conserved with chromosomal distribution. They could be divided into three groups based on exon-intron structure, and cultivated tomato contained only two of these subclades. A number of hormonal, light and abiotic stress-responsive cis-regulatory elements were identified from the promoter of the tomato SRO gene, and they also interacted with a variety of stress-responsive proteins and microRNAs. RNA-seq analysis showed that SRO genes were widely expressed in different tissues and developmental stages of tomato, with significant tissue-specific features. Expression analysis also showed that SRO genes respond significantly to high temperature and salt stress and mediate the tomato hormone regulatory network. These results provide a theoretical basis for further investigation of the functional expression of tomato SRO genes and provide potential genetic resources for tomato resistance breeding.

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“…Subsequently, five homologs (named as AtSRO1-5) of AtRCD1 were also identified in Arabidopsis. AtSRO1 is the closest homolog of AtRCD1, both of them contained WWE domain at the N-terminus and possessed similar expression patterns, but they play unequally redundant and independent roles in certain developmental processes and the response to various stresses [14,[16][17][18]. For example, sro1-1 mutants displayed only unconspicuous morphological phenotypes, while rcd1-3 sro1-1 double mutants showed even more extreme phenotypes than rcd1-3 [13,14].…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning, Characterization, and Expression Analysis of SIMILAR TO RCD-ONE (SRO) Family Genes Responding to Abiotic and Biotic Stress in Cucumber

Xiao

ZHOU²,

Zhu³

et al. 2022

Horticulturae

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SIMILAR TO RCD-ONE (SRO) is a plant-specific small protein family that controls many biological processes including physiological development and stress responses. The SRO gene family has been studied in several plant species, but no detailed characterization and expression profiles of this important gene family were performed in cucumber. In this study, we characterize the SRO genes in cucumber, and determined their transcript levels in various tissues and under exposure to diverse biotic and abiotic stressors. Four SRO genes (named as CsSRO1–CsSRO4) were identified and isolated, which were distributed on three different chromosomes. Gene duplication analysis showed that only one pair of segmental duplication event was identified, but no tandem duplication events were detected. All CsSROs consist of the PARP domain and a C-terminal RST domain, while the N-terminal WWE domain was only present in CsSRO2 and CsSRO4. SROs from 15 plant species are divided into two groups (I and II), and group I can be further divided into four subgroups (Ia to Id) according to the phylogenetic tree. The conserved motif and gene structure analyses showed that SROs within the same branch of the phylogenetic tree have analogous conserved motifs configuration and gene structures. However, SRO genes possessed variable numbers of introns in different subgroups, which may affect the evolution of new family members. RNA-Seq data and qRT-PCR results showed that the four CsSRO genes have distinct expression pattern in various tissues and under diverse stresses, suggesting their multiple functions in plant growth and stress responses. The findings provide a basis for further research aiming at functional characterization of the regulatory mechanism to reveal the roles of CsSRO genes in developmental and stress-related processes of cucumber.

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Structure and function analysis of Arabidopsis thaliana SRO protein family

Cited by 11 publications

References 0 publications

Genome-Wide Identification and Analysis of SRO Gene Family in Chinese Cabbage (Brassica rapa L.)

Genome-Wide Identification and Analysis of SRO Gene Family in Chinese Cabbage (Brassica rapa L.)

Genome-Wide Identification and Evolutionary Analysis of the SRO Gene Family in Tomato

Molecular Cloning, Characterization, and Expression Analysis of SIMILAR TO RCD-ONE (SRO) Family Genes Responding to Abiotic and Biotic Stress in Cucumber

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