Structure and expression of two members of the d4 gene family in mouse

Мерцалов, И. Б.; Куликова, Д. А.; Alimova-Kost, Maria V.; Ninkina, Natalia; Korochkin, L. I.; Buchman, Vladimir L.

doi:10.1007/s003350010014

Cited by 12 publications

(5 citation statements)

References 10 publications

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“…In the mouse, Dpf1 (Neud4) is expressed predominantly in the brain, and may have an important role in developing neurons through regulation of cell survival as a neurospecific transcription factor (Lessard et al 2007). Dpf2 (ubi-d4/requiem) is ubiquitously expressed (Mertsalov et al 2000) and implicated to be required for cell death after deprivation of trophic factors (Gabig et al 1994).…”

Section: Dpf3 Is a Muscle Expressed Member Of The D4 Zinc And Doublmentioning

confidence: 99%

Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex

Lange¹,

Kaynak²,

Förster³

et al. 2008

Genes Dev.

204

213

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Chromatin remodeling and histone modifications facilitate access of transcription factors to DNA by promoting the unwinding and destabilization of histone-DNA interactions. We present DPF3, a new epigenetic key factor for heart and muscle development characterized by a double PHD finger. DPF3 is associated with the BAF chromatin remodeling complex and binds methylated and acetylated lysine residues of histone 3 and 4. Thus, DPF3 may represent the first plant homeodomains that bind acetylated lysines, a feature previously only shown for the bromodomain. During development Dpf3 is expressed in the heart and somites of mouse, chicken, and zebrafish. Morpholino knockdown of dpf3 in zebrafish leads to incomplete cardiac looping and severely reduced ventricular contractility, with disassembled muscular fibers caused by transcriptional deregulation of structural and regulatory proteins. Promoter analysis identified Dpf3 as a novel downstream target of Mef2a. Taken together, DPF3 adds a further layer of complexity to the BAF complex by representing a tissue-specific anchor between histone acetylations as well as methylations and chromatin remodeling. Furthermore, this shows that plant homeodomain proteins play a yet unexplored role in recruiting chromatin remodeling complexes to acetylated histones.[Keywords: Heart and skeletal muscle development and function; PHD finger; BAF chromatin remodeling complex; SMARCD3-BAF60; acetylated and methylated histones; Mef2] Supplemental material is available at http://www.genesdev.org.

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Section: Dpf3 Is a Muscle Expressed Member Of The D4 Zinc And Doublmentioning

confidence: 99%

Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex

Lange¹,

Kaynak²,

Förster³

et al. 2008

Genes Dev.

204

213

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“…In the mouse, BAF45a is essential for the maintenance of hematopoietic stem cells (Krasteva et al., 2017) and for the self-renewal of neural progenitors and is replaced by BAF45b / c as neural progenitors differentiate (Kadoch and Crabtree, 2015), whereas BAF45c is critical for heart and muscle development (Lange et al., 2008). BAF45d , also called Dpf2 , is the only ubiquitously expressed BAF45 subunit (Mertsalov et al., 2000) and, so far, has been implicated in the programmed cell death response after deprivation of interleukin-3 from myeloid cells (Gabig et al., 1994). However, the biochemical interaction of DPF2 with pluripotency TFs in ESCs (Pardo et al., 2010, van den Berg et al., 2010) suggests a function of this BAF subunit in pluripotent cells, which has not been examined to date.…”

Section: Introductionmentioning

confidence: 99%

The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation

et al. 2019

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Summary BAF complexes are composed of different subunits with varying functional and developmental roles, although many subunits have not been examined in depth. Here we show that the Baf45 subunit Dpf2 maintains pluripotency and ESC differentiation potential. Dpf2 co-occupies enhancers with Oct4, Sox2, p300, and the BAF subunit Brg1, and deleting Dpf2 perturbs ESC self-renewal, induces repression of Tbx3, and impairs mesendodermal differentiation without dramatically altering Brg1 localization. Mesendodermal differentiation can be rescued by restoring Tbx3 expression, whose distal enhancer is positively regulated by Dpf2-dependent H3K27ac maintenance and recruitment of pluripotency TFs and Brg1. In contrast, the PRC2 subunit Eed binds an intragenic Tbx3 enhancer to oppose Dpf2-dependent Tbx3 expression and mesendodermal differentiation. The PRC2 subunit Ezh2 likewise opposes Dpf2-dependent differentiation through a distinct mechanism involving Nanog repression. Together, these findings delineate distinct mechanistic roles for specific BAF and PRC2 subunits during ESC differentiation.

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“…Members of the evolutionarily conserved d4 family of transcription factors DPF1/ neuro‐d4 , DPF2 /requiem/ubi‐d4 and DPF3/ cer‐d4 play important roles in programmed cell death and development (Buchman et al, ; Gabig, Mantel, Rosli, & Crean, ; Lange et al, ; Lim, Seah, Mantalaris, Yap, & Wong, ; Tando et al, ; Wu et al, ). DPF1/ neuro‐d4 and DPF3/ cer‐d4 were originally described as transcription factors that are expressed only in the central and peripheral nervous systems of mice (Buchman et al, ; Kulikova, Mertsalov, & Simonova, ; Lessard et al, ; Mertsalov et al, ). In contrast, DPF2 /requiem/ubi‐d4 is expressed in all tissues and organs of mice and is required for the apoptotic response upon IL‐3 withdrawal in myeloid cells (Gabig et al, ).…”

Section: Introductionmentioning

confidence: 99%

DPFF‐1 transcription factor deficiency causes the aberrant activation of MPK‐1 and meiotic defects in the Caenorhabditis elegans germline

Villanueva-Chimal

Salinas

Fernández-Cárdenas

et al. 2017

Genesis

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The d4 family of transcription factors consists of three members in mammals. DPF1/neuro-d4 is expressed mainly in neurons and the peripheral nervous system, and is important for brain development. DPF2/requiem/ubi-d4 is expressed ubiquitously and presumably functions as an apoptotic factor, especially during the deprivation of trophic factors. DPF3/cer-d4 is expressed in neurons and in the heart, and is important for heart development and function in zebrafish. In Drosophila, there is only one member, dd4, whose function is still unknown, but it is expressed in many tissues and is particularly abundant in the brain of developing embryos and in adults. Here, we present DPFF-1, the only member of this family of proteins in the nematode C. elegans. DPFF-1 is similar to its mammalian homolog DPF2/requiem/ubi-d4 because it is ubiquitously expressed during embryogenesis and in adult tissues, and because it is important for the induction of germ cell apoptosis during stress. Here, we show that dpff-1 null mutant animals produce less progeny than wild-type nematodes, presumably due to meiotic defects. Gonads of dpff-1 deficient animals showed more germ cells in pachytene and overexpressed the P-MPK-1 signal. Additionally, these animals presented higher levels of p53-induced germ cell apoptosis than wild-type animals. Furthermore, we observed that dpff-1 deficient animals are more sensitive to heat shock. This is the first report showing that the d4 family of transcription factors could be involved in meiosis and stress protection.

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Structure and expression of two members of the d4 gene family in mouse

Cited by 12 publications

References 10 publications

Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex

Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex

The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation

DPFF‐1 transcription factor deficiency causes the aberrant activation of MPK‐1 and meiotic defects in the Caenorhabditis elegans germline

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