Structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in Arabidopsis thaliana

Leutwiler, Leslie S.; Meyerowitz, Elliot M.; Tobin, Elaine M.

doi:10.1093/nar/14.10.4051

Cited by 240 publications

(193 citation statements)

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“…The CAB2-specific probe is a 22-base oligonucleotide from the specific RNA leader of the CAB2 transcript (Leutwiler et al, 1986), 5Ј-ATGACTAACTTGTGAG-TGAGA-3Ј. The CAB1 probe is a 42-base oligonucleotide from the CAB1 mRNA leader region, 5Ј-CAGCAC-AAAGTAAACGTTTAAGATTTGTTGTTGTAAGCCAA-3Ј.…”

Section: Rna Gel Blot Analysismentioning

confidence: 99%

“…The alcohol dehydrogenase (ADH) gene probe is a 0.6-kb SalI-NotI fragment of the 194E11T7 expressed sequence tag clone, and the albumin (ALB) 2S gene probe is a 0.7-kb SalI-NotI fragment of the 107I7T7 expressed sequence tag clone. As loading control for total RNA, we used an 18S rDNA fragment cloned from Arabidopsis (Takahashi et al, 1995).The CAB2-specific probe is a 22-base oligonucleotide from the specific RNA leader of the CAB2 transcript (Leutwiler et al, 1986), 5Ј-ATGACTAACTTGTGAG-TGAGA-3Ј. The CAB1 probe is a 42-base oligonucleotide from the CAB1 mRNA leader region, 5Ј-CAGCAC-AAAGTAAACGTTTAAGATTTGTTGTTGTAAGCCAA-3Ј.…”

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An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals

et al. 1998

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A new mutant called psi2 (for p hytochrome si gnaling) was isolated by screening for elevated activity of a chlorophyll a / b binding protein-luciferase ( CAB2-LUC ) transgene in Arabidopsis. This mutant exhibited hypersensitive induction of CAB1 , CAB2 , and the small subunit of ribulose-1,5-bisphosphate carboxylase ( RBCS ) promoters in the very low fluence range of red light and a hypersensitive response in hypocotyl growth in continuous red light of higher fluences. In addition, at high-but not low-light fluence rates, the mutant showed light-dependent superinduction of the pathogenrelated protein gene PR-1a and developed spontaneous necrotic lesions in the absence of any pathogen. Expression of genes responding to various hormone and environmental stress pathways in the mutant was not significantly different from that of the wild type. Analysis of double mutants demonstrated that the effects of the psi2 mutation are dependent on both phytochromes phyA and phyB. The mutation is recessive and maps to the bottom of chromosome 5. Together, our results suggest that PSI2 specifically and negatively regulates both phyA and phyB phototransduction pathways. The induction of cell death by deregulated signaling pathways observed in psi2 is reminiscent of retinal degenerative diseases in animals and humans. INTRODUCTIONBecause light provides the energy source for photosynthesis, plants must adapt to changes in ambient light quantity and quality to optimize the efficiency of this important process. The results of this adaptation are apparent: almost every step of plant development, from seed germination to fruit maturation, is dependent on light. Moreover, light regulates the circadian rhythm, the adaptative size modification of tissue and organs, and the expression of photosynthetic genes and many other known and unknown genes (Terzaghi and Cashmore, 1995).To date, what we know about light information processing and signal transduction concerns the nature and function of photoreceptors. A family of photoreceptors called phytochromes has been shown to be involved in the perception of red and far-red light. Their biochemical and molecular properties and physiological roles are now well described (reviewed in Quail et al., 1995;Smith, 1995). In addition, a longpostulated UV and blue light receptor (cryptochrome) was recently characterized by Ahmad and Cashmore (1993). Mutants lacking one or several of these receptors have provided useful information concerning the role of individual photoreceptors and their interaction in transducing light signals (reviewed in Barnes et al., 1997).Much less is known concerning molecules that link light perception to gene activation. The CONSTITUTIVE PHOTO-MORPHOGENIC/DEETIOLATED/FUSCA (COP/DET/FUS) class of proteins has been suggested to play an important role in regulating the repression of light-inducible genes and the maintenance of skotomorphogenesis (Chory et al., 1989). For some of these proteins, the genes have been cloned and include COP1 (Deng et al., 1992), DET1 (Pepper et al., ...

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Section: Rna Gel Blot Analysismentioning

confidence: 99%

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An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals

et al. 1998

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“…Filter hybridizations were performed at 65OC in a solution containing 7% SDS, 500 mM NaH2P04, pH 7.2,1% BSA, and 1 mM EDTA. The DNA probes used are as follows: a 0.55-kb DNA fragment (generated by polymerase chain reaction) corresponding to the entire open reading frame of the Arabidopsis small subunit of ribulose-l,5-bisphosphate carboxylase rbcS gene (Krebbers et al, 1988); a 0.5-kb BamHI-Sstl DNA fragment corresponding to the coding region of the Arabidopsis chlorophyll alb (cab3) gene (Leutwiler et al, 1986); a 1.5-kb Hincll-ECORI DNA fragment containing the Arabidopsis ferredoxin type A (fedA) gene (Somers et al, 1990); a 1.2-kb Bglll-Xbal DNA fragment containing most of the coding region of a spinach chloroplast plastid @sbA) gene (Zurawski et al, 1982); and a 2-kb Hindlll fragment containing the entire p-glucuronidase (GUS) coding sequence used for the cabl promoter-GUS constructs (Deng et al, 1991). For all probes, -200 ng of each purified DNA fragment was labeled to high specific activity (-2 to 3 x 105 cpmlng DNA) by random oligomer priming, denatured by boiling for 5 min, and used for hybridization.…”

Section: Rna Analysismentioning

confidence: 99%

A New Class of Arabidopsis Constitutive Photomorphogenic Genes Involved in Regulating Cotyledon Development.

1993

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Light signals have profound effects on morphogenesis of hypocotyls and cotyledons of Arabidopsis seedlings, but the mechanisms by which light signals are transduced and integrated to control these processes are poorly understood.We report here the identification of a new class of constitutive photomorphogenic (cop) mutants, cop2, cop3, and cop4, in which dark-grown seedlings have open and enlarged cotyledons resembling those of light-grown wild-type seedlings. The epistatic relationships of these three mutations to previously characterized phytochrome-deficient mutations suggest that C O R , COP3, and COP4 may act downstream of phytochrome in the light regulatory pathway. Mutations in each of the three loci.alleviate the normal inhibition of cell-type differentiation, cell enlargement, and lateral cell division obsenred in cotyledons of dark-grown wild-type seedlings, but do not affect plastid differentiation. The cop4 mutation also leads to high-leve1 dark expression of nuclear, but not plastid-encoded, light-inducible genes. We further show that for the nuclear cabl gene encoding a chlorophyll alb binding protein of the photosynthetic light-harvesting complex, activation in dark-grown cop4 mutants is achieved by modulation of promoter activity. Interestingly, COP4 modulates cabl promoter activity through a pathway distinct from that of COPl and COP9. Furthermore, cop4 mutants are detecrive in both root and shoot gravitropic responses, indicating that the COP4 locus may be involved in both light-signaling and gravity-sensing processes.

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“…In Arabidopsis, several multiple loci exist for genes of Lhcb1 and Lhcb2. There are at least five (Lhcb1.1-Lhcb1.5) (AT1G29920, AT1G29910, AT1G29930, AT2G34430, AT2G34420), and three (Lhcb2.1-Lhcb2.3) (AT2G05100, AT2G05070, AT3G27690) multiple loci encoding Lhcb1 and Lhcb2 respectively in A. thaliana [3][4][5] (Table 1). The amino acid sequences of these individual Lhcb1 and Lhcb2 proteins are slightly different.…”

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Genome-wide analysis of the family of light-harvesting chlorophyll a/b-binding proteins in Arabidopsis and rice

Umate

2010

Plant Signaling & Behavior

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Structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in Arabidopsis thaliana

Cited by 240 publications

References 24 publications

An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals

An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals

A New Class of Arabidopsis Constitutive Photomorphogenic Genes Involved in Regulating Cotyledon Development.

Genome-wide analysis of the family of light-harvesting chlorophyll a/b-binding proteins in Arabidopsis and rice

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