Structure and expression of glutathione S-transferase genes from the midgut of the Common cutworm, Spodoptera litura (Noctuidae) and their response to xenobiotic compounds and bacteria

Huang, Yingwei; Xu, Zhibin; Lin, Xianyu; Feng, Qiang; Zheng, Sichun

doi:10.1016/j.jinsphys.2011.05.001

Cited by 91 publications

(91 citation statements)

References 35 publications

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“…For the Omega-class GSTs, this protective role against oxidative stress is mediated by DHA reductase and thiol transferase activities (Yamamoto et al 2011), whereas other insect GSTs do not exhibit these activities. Currently, lack of knowledge of endogenous insect GST substrates makes it difficult to elucidate the precise roles of different insect GST classes, and not all insect GSTs are involved in detoxification and antioxidative stress (Huang et al, 2011). In this study, several antioxidant response elements, such as HSF and AP-1, were predicted in the 5′-flanking region of AccGSTO2.…”

Section: Discussionmentioning

confidence: 97%

“…These results suggest that the function of GSTOs in insects is conserved. In general, the elevated mRNA levels of GSTO are always correlated with their known roles (Huang et al 2011;Yamamoto et al 2011;Wan et al 2009). This appears to the case in C. elegans, where specific silencing of the GSTO1-1 by RNAi created worms with an increased sensitivity to several pro-oxidants, arsenite, and heat shock (Burmeister et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…GSTs catalyse the conjugation of reduced glutathione with compounds containing an electrophilic centre, which forms more soluble, nontoxic peptide derivatives that can be excreted from cells. Therefore, GSTs play central roles in the detoxification of endogenous and xenobiotic compounds, including drugs, herbicides and insecticides (Huang et al 2011). GSTs also participate in the biosynthesis and intracellular transport of hormones and in the protection against oxidative stress (Hayes et al 2005;Cnubben et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress

Zhang

Yan

et al. 2013

Cell Stress and Chaperones

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Oxidative stress may be the most significant threat to the survival of living organisms. Glutathione S-transferases (GSTs) serve as the primary defences against xenobiotic and peroxidative-induced oxidative damage. In contrast to other well-defined GST classes, the Omega-class members are poorly understood, particularly in insects. Here, we isolated and characterised the GSTO2 gene from Apis cerana cerana (AccGSTO2). The predicted transcription factor binding sites in the AccGSTO2 promoter suggested possible functions in early development and antioxidant defence. Real-time quantitative PCR (qPCR) and western blot analyses indicated that AccGSTO2 was highly expressed in larvae and was predominantly localised to the brain tissue in adults. Moreover, AccGSTO2 transcription was induced by various abiotic stresses. The purified recombinant AccGSTO2 exhibited glutathione-dependent dehydroascorbate reductase and peroxidase activities. Furthermore, it could prevent DNA damage. In addition, Escherichia coli overexpressing AccGSTO2 displayed resistance to long-term oxidative stress exposure in disc diffusion assays. Taken together, these results suggest that AccGSTO2 plays a protective role in counteracting oxidative stress.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress

Zhang

Yan

et al. 2013

Cell Stress and Chaperones

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“…The nested gene-specific primers (Table 1) for the TAIL-PCR were designed according to the core region. The TAIL-PCR was performed as described by Huang (Huang et al 2011). The amplified PCR products were subjected to sequencing.…”

Section: Cloning Of the Phytase Genementioning

confidence: 99%

Microbial diversity of mangrove sediment in Shenzhen Bay and gene cloning, characterization of an isolated phytase-producing strain of SPC09 B. cereus

Zhang

Liao

et al. 2015

Appl Microbiol Biotechnol

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Phytases hydrolyze phytate to release inorganic phosphate, which decreases the requirement for phosphorus in fertilizers for crops and thus reduces environmental pollutants. This study analyzed microbial communities in rhizosphere sediment, collected in September 2012 from Shenzhen Bay, Guangdong, China, using high-throughput pyrosequencing; the results showed that the dominant taxonomic phyla were Chloroflexi, Firmicutes, and Proteobacteria, and the proportion of the beneficial bacteria, Bacillus, was 4.95 %. Twenty-nine culturable, phytase-producing bacteria were isolated, their phosphorus solubilization capacity was analyzed, and they were taxonomically characterized. Their phylogenetic placement was determined using 16S ribosomal RNA (rRNA) gene sequence analysis. The result shows that most of the isolates are members of the order Bacillales, although seven strains of Enterobacteriales, two strains of Pseudomonadales, and one strain of Oceanospirillales were also identified. The phytase gene was cloned from SPC09, Bacillus cereus, which showed the highest phosphorus solubilizing ability among the isolated strains. The gene encoded a primary translation product of 335 amino acids. A construct including the 1005-nt ORF fragment, Bc-phy, was transformed into Escherichia coli. The recombinant phytase was produced and purified, which revealed the temperature optima at 60 °C and pH optima at 6.5. The assessment by quantitative PCR (qPCR) showed an abundance of bacteria containing the Bc-phy gene; the level was generally higher in the mangrove forest than in the tidal flats and in surface soil compared to bottom soil, and the highest value was obtained in June. Herein, we report on the cloning, characterization, and activity of a novel phytase isolated from a mangrove system.

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“…Insect GSTs are known to be specifically or preferentially expressed into major detoxification organs such as fat body, midgut but also in epidermis and in Malpighian tubules (Huang et al, 2011). However, the expression of GSTs as well as their activities have been also highlighted in the olfactory organs of several insect species.…”

Section: Introductionmentioning

confidence: 99%

Glutathione-S-Transferases in the Olfactory Organ of the Noctuid Moth Spodoptera littoralis, Diversity and Conservation of Chemosensory Clades

et al. 2018

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Glutathione-S-transferases (GSTs) are conjugating enzymes involved in the detoxification of a wide range of xenobiotic compounds. The expression of GSTs as well as their activities have been also highlighted in the olfactory organs of several species, including insects, where they could play a role in the signal termination and in odorant clearance. Using a transcriptomic approach, we identified 33 putative GSTs expressed in the antennae of the cotton leafworm Spodoptera littoralis. We established their expression patterns and revealed four olfactory-enriched genes in adults. In order to investigate the evolution of antennal GST repertoires in moths, we re-annotated antennal transcripts corresponding to GSTs in two moth and one coleopteran species. We performed a large phylogenetic analysis that revealed an unsuspected structural—and potentially functional—diversity of GSTs within the olfactory organ of insects. This led us to identify a conserved clade containing most of the already identified antennal-specific and antennal-enriched GSTs from moths. In addition, for all the sequences from this clade, we were able to identify a signal peptide, which is an unusual structural feature for GSTs. Taken together, these data highlight the diversity and evolution of GSTs in the olfactory organ of a pest species and more generally in the olfactory system of moths, and also the conservation of putative extracellular members across multiple insect orders.

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Structure and expression of glutathione S-transferase genes from the midgut of the Common cutworm, Spodoptera litura (Noctuidae) and their response to xenobiotic compounds and bacteria

Cited by 91 publications

References 35 publications

A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress

A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress

Microbial diversity of mangrove sediment in Shenzhen Bay and gene cloning, characterization of an isolated phytase-producing strain of SPC09 B. cereus

Glutathione-S-Transferases in the Olfactory Organ of the Noctuid Moth Spodoptera littoralis, Diversity and Conservation of Chemosensory Clades

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