Structure and expression of genes coding for structural proteins of the plant cell wall

Jose, Matilde; Puigdomènech, Pere

doi:10.1111/j.1469-8137.1993.tb03881.x

Cited by 72 publications

(37 citation statements)

References 176 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The 500-bp region upstream to the transcription start site of PM-HRGP showed 60% homology with the upstream element of the maize extensin gene. The upstream regulatory sequences of the HRGP genes responsible for tissue specificity, wound inducibility and activation during infection have been extensively studied in different species such as maize, carrot, tobacco and oilseed rape (Jose and Puigdomenech 1993). Further studies directed toward the identification of the regulatory elements present in the PM-HRGP gene could lead to a better understanding of its role during stress, pathogen attack and elicitor treatment.…”

Section: Actamentioning

confidence: 99%

Characterization of a hydroxyproline-rich glycoprotein in pearl millet and its differential expression in response to the downy mildew pathogen Sclerospora graminicola

et al. 2011

View full text Add to dashboard Cite

A monoclonal antibody, JIM 20, derived against an extensin type of hydroxyproline-rich glycoprotein (HRGP) from pea, showed high affinity for HRGP in pearl millet [Pennisetum glaucum (L.) R. Br.]. Electrophoretic separation of Tris-SDS extracted proteins from suspension cells of pearl millet revealed a range of PM-HRGP polypeptides having a glycan epitope, which reacted with JIM 20. A high molecular mass band, probably an HRGP aggregate or polymer, and a few low molecular mass polypeptides were recognized by JIM 20 during Western blot analysis. Treatment of pearl millet suspension cells with hydrogen peroxide in the presence of an endogenous peroxidase resulted in insolubilization of HRGP polypeptides with molecular weights between 45 and 33 kDa. To investigate the gene coding for an extensin type of HRGP, a fosmid-based genomic library of pearl millet having a fourfold genome coverage was constructed. A partial sequence of 378 bp of an HRGP gene was obtained by PCR amplification of pearl millet DNA with a primer pair designed from the conserved regions of monocotyledon extensin type of HRGPs. Screening the genomic library using the homologous probe developed from the 378-bp PCR product resulted in the isolation of five fosmid clones. Restriction mapping of these fosmids resulted in an 11.8-kb region around an HRGP gene in pearl millet. The newly characterized gene, PM-HRGP, had all the characteristic features of a monocotyledon extensin type of HRGP. An intron at the 3 0 untranslated region of the gene was identified by cDNA cloning. Differential expression of the PM-HRGP gene was observed during compatible and incompatible interactions of pearl millet with the downy mildew pathogen Sclerospora graminicola (Sacc) Schroet. Induced expression of the gene was observed only in case of an incompatible interaction.

show abstract

Section: Actamentioning

confidence: 99%

Characterization of a hydroxyproline-rich glycoprotein in pearl millet and its differential expression in response to the downy mildew pathogen Sclerospora graminicola

et al. 2011

View full text Add to dashboard Cite

show abstract

“…As well as being a 'sense organ' of the plant, acting to trigger changes within the plant body, the structure of the cell wall itself changes due to attack by pathogens, or damage by external stresses. Structural proteins such as the hydroxyproline-rich glycoproteins ( H R G P s ) or extensins; the proline rich proteins (PRPs); and the glycine-rich proteins (GRPs) are known to be induced in response to pathogen infection and physical damage, and it is therefore likely that they play an important primary role in plant protection [ 1,4,9,18 ].…”

Section: Introductionmentioning

confidence: 99%

A gene for Brassica napus extensin is differentially expressed on wounding

1996

View full text Add to dashboard Cite

We have analysed the expression of the endogenous extensin genes in Brassica napus, using northern hybridisation and dot blotting. In the unstressed plant, the extA gene is only expressed in the root, expression in the leaf, petiole and stem being absent. We have found that wounding dramatically alters this normal pattern of expression. Expression in wounded leaf is seen after 36 h, in wounded petioles after 11 h and in wounded stem after 17 h. Differences in the amount of extensin mRNA accumulated are also seen: wounded petiole accumulating extensin message to a level higher than the leaf or the stem. Inhibitors of ethylene biosynthesis greatly delay the onset of accumulation of extensin mRNA in wounded tissues. Wounding the root causes the level of extensin message to decline with time, until levels below the limit of non-specific hybridisation are reached 11 h after wounding. Thus, application of the wounding stimulus results in the accumulation of extensin gene transcripts to different degrees and at different times in the aerial parts of the plant, and results in a decline in the same transcripts in the roots. Extensin transcript accumulation as a result of wounding is also dependent on the age of the tissue; high levels of message are seen in old wounded leaves, while expression in young wounded leaves is absent.

show abstract

“…Extensins are proteins that are secreted into the primary wall of plant cells (Jose and Puigdomenech, 1993;Showalter, 1993;Kieliszewski and Lamport, 1994;Cassab, 1998;Sommer-Knudsen et al, 1998;Jamet et al, 2000;Showalter et al, 2010;Lamport et al, 2011). The pentapeptide motif Ser(Pro) 4 occurs many times throughout the primary structure of extensins and comprises their structural characteristic feature.…”

Section: Introductionmentioning

confidence: 99%

Histochemical map of the ectopic expression of the Arabidopsis atExt1 extensin gene in transgenic tobacco

Merkouropoulos

Shirsat

2012

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Histochemical analysis of transgenic tobacco plants harboring the promoter of the Arabidopsis extensin gene atExt1 fused to the β-glucuronidase gene coding sequence demonstrated expression of the transgene in stem tissues. The transgene was expressed in cells of the internal and external phloem at the nodal and internodal regions of the older parts of mature stems. In younger parts of the same stem, expression of the transgene was slightly modified: expression was detected in the internal phloem in the internodal areas. In the nodal areas, the phloem tissue and the surrounding parenchyma were stained, demonstrating transgene expression. Expression was also seen in the phloem of the inflorescence; it was non-specific at the junctions of the flowers to the inflorescence. β-glucuronidase (reporter gene) staining was also observed in the pollen grains and at the base of the corolla.

show abstract

Structure and expression of genes coding for structural proteins of the plant cell wall

Cited by 72 publications

References 176 publications

Characterization of a hydroxyproline-rich glycoprotein in pearl millet and its differential expression in response to the downy mildew pathogen Sclerospora graminicola

Characterization of a hydroxyproline-rich glycoprotein in pearl millet and its differential expression in response to the downy mildew pathogen Sclerospora graminicola

A gene for Brassica napus extensin is differentially expressed on wounding

Histochemical map of the ectopic expression of the Arabidopsis atExt1 extensin gene in transgenic tobacco

Contact Info

Product

Resources

About