Structure and Expression of Endogenous Retroviral Sequences in the Permanent LMH Chicken Cell Line

Ronfort, Corinne; Chebloune, Yahia; Cosset, François‐Loïc; Fauré, Christine; Nigon, Victor; Verdier, Gérard

doi:10.3382/ps.0740127

Cited by 5 publications

(2 citation statements)

References 33 publications

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“…There was a numerical 21.1% reduction in nitrogen excreted when 15% CP supplemented with Met and Lys in the present study. These results are in agreement with several studies reporting that low-protein amino acid-supplemented diets had no negative effect on broiler performance, while reducing nitrogen excretion by 33.6% [ 24 , 43 , 52 , 54 , 55 ]. The reduction of nitrogen excretion also influences ammonia volatilization in poultry houses: supplementing amino acids in low-protein diets reduced ammonia volatilization by 45% in poultry [ 4 , 5 , 56 ].…”

Section: Discussionsupporting

confidence: 93%

“…Some studies have shown that broilers eat more feed to satisfy their protein or amino acid needs under hot climate [ 9 , 50 , 51 ]. The feed conversion ratios in this study are similar to other studies showing that there were no significant differences in feed intake and efficiency of feed use among birds fed a low protein and a high-protein diets [ 8 , 51 , 52 , 53 ].…”

Section: Discussionsupporting

confidence: 89%

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Multiple Amino Acid Supplementations to Low-Protein Diets: Effect on Performance, Carcass Yield, Meat Quality and Nitrogen Excretion of Finishing Broilers under Hot Climate Conditions

Attia

Bovera

Wang

et al. 2020

Animals

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The objective of this study was to evaluate the effect of low-protein diets with amino acid supplementation on growth performance, carcass yield, meat quality and nitrogen excretion of broilers raised under hot climate conditions during the finisher period. In trial 1, broilers from 28 to 49 days of age were fed 18% crude protein (CP) as a positive control or 15% CP supplemented with (1) DL-methionine (Met) + L-lysine (Lys), (2) Met + Lys + L-Arginine (Arg), or (3) Met + Lys + L-Valine (Val). In trial 2, broilers from 30 to 45 days of age, were fed an 18% CP diet as a positive control or 15% CP supplemented with Met, Lys, Arg, Val, L-Isoleucine (Ile) or combination with glycine (Gly) and/or urea as nitrogen sources: (1) Met + Lys, (2) Met + Lys + Arg, (3) Met + Lys + Val, (4) Met + Lys + Ile, (5) Met + Lys + Arg +Val + Ile + Gly, and (6) Met+ Lys + Arg + Val + Ile + Gly + urea. Protein use was improved by feeding low-protein amino acid-supplemented diets as compared to the high-protein diet. Feeding 15% crude protein diet supplemented with only methionine and lysine had no negative effects on carcass yield, CP, total lipids and moisture% of breast meat while decreasing nitrogen excretion by 21%.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 89%

Multiple Amino Acid Supplementations to Low-Protein Diets: Effect on Performance, Carcass Yield, Meat Quality and Nitrogen Excretion of Finishing Broilers under Hot Climate Conditions

Attia

Bovera

Wang

et al. 2020

Animals

View full text Add to dashboard Cite

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An improved self-deleting retroviral vector derived from avian leukemia and sarcoma virus

et al. 2008

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We have previously developed a self-deleting avian leukosis and sarcoma virus (ALSV)- based retroviral vector carrying an additional attachment (att) sequence. Resulting proviruses underwent deletion of viral sequences and were flanked either by two LTRs (LTRs proviruses) or by the additional att sequence and the 3' LTR (att proviruses). Herein, we have tried to increase (1) the self-deleting properties of this vector, either by raising the selection pressure applied on target cells or by optimizing the size of the internal att sequence, (2) the titer of the vector by deleting or inverting some viral sequences. Moreover, a new type of provirus flanked by att sequences at each end was isolated. Finally, under specific conditions, 100% of proviruses had internal sequences deleted, and as many as 92-100% of proviruses were no longer mobilizable by a replication-competent virus. The inactivation procedure achieved here might improve the biosafety of retroviral vectors.

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Quantification of HIV-based lentiviral vectors: influence of several cell type parameters on vector infectivity

2011

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A human immunodeficiency virus type (HIV-1)-based lentiviral vector pseudotyped with the vesicular stomatitis virus envelope glycoprotein and encoding the GFP reporter gene was used to evaluate different methods of lentiviral vector titration. GFP expression, viral DNA quantification and the efficiency of vector DNA integration were assayed after infection of conventional HIV-1-permissive cell lines and human primary adult fibroblasts with the vector. We found that vector titers based on GFP expression determined by flow cytometry may vary by more than 50-fold depending on the cell type and the promoter-cell combination used. Interestingly, we observed that the viral integration process in primary HDFa cells was significantly more efficient compared to that in SupT1 or 293T cells. We propose that determination of the amount of integrated viral DNA by quantitative PCR be used in combination with the reporter gene expression assay.

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Structure and Expression of Endogenous Retroviral Sequences in the Permanent LMH Chicken Cell Line

Cited by 5 publications

References 33 publications

Multiple Amino Acid Supplementations to Low-Protein Diets: Effect on Performance, Carcass Yield, Meat Quality and Nitrogen Excretion of Finishing Broilers under Hot Climate Conditions

Multiple Amino Acid Supplementations to Low-Protein Diets: Effect on Performance, Carcass Yield, Meat Quality and Nitrogen Excretion of Finishing Broilers under Hot Climate Conditions

An improved self-deleting retroviral vector derived from avian leukemia and sarcoma virus

Quantification of HIV-based lentiviral vectors: influence of several cell type parameters on vector infectivity

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