Structure and expression of a novel gene family showing male germline specific expression in <i> Drosophila melanogaster</i>

Cara, Francesca Di; Morra, R.; Cavaliere, Daniela; Sorrentino, Anna; Simone, A. De; Polito, Catello; Digilio, A.

doi:10.1111/j.1365-2583.2006.00688.x

Cited by 10 publications

(8 citation statements)

References 33 publications

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“…Northern blot was carried out according to Di Cara et al 2006. The 32 P labeled ix probe was prepared by singlestranded PCR with primer Rev CDS (underlined nucleotides 810-830 in Fig.…”

Section: Northern Analysismentioning

confidence: 99%

Cloning and functional characterization of the intersex homologous gene in the pest lepidopteron Maruca vitrata

Cavaliere

Cara²,

Polito³

et al. 2009

Int. J. Dev. Biol.

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The intersex (ix) gene works in concert with doublesex (dsx) at the bottom of the sexdetermination hierarchy to control somatic sexual differentiation in Drosophila melanogaster females. Here we report the isolation and characterization of the Drosophila intersex (ix) homologue in the pest lepidopteron Maruca vitrata (Mvix). The Mvix gene exhibits major complexity with respect to the Drosophila homolog. It is expressed in males and females and its pre-mRNA is subject to differential splicing events which affect both the protein coding and the non-coding regions. Moreover, Northern blot experiments revealed the presence of a femalespecific transcript in pupae RNA, which appears to be the first described sex specific transcript of ix homologs characterized to date. The expression of Mvix cDNA in D.melanogaster transgenic flies indicates that the MvIX product, which shares a relatively high degree of homology with the D.melanogaster IX protein, is able to partially rescues the Drosophila mutant phenotype.

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“…Northern blot was carried out according to Di Cara et al 2006. The 32 P labeled ix probe was prepared by singlestranded PCR with primer Rev CDS (underlined nucleotides 810-830 in Fig.…”

Section: Northern Analysismentioning

confidence: 99%

Cloning and functional characterization of the intersex homologous gene in the pest lepidopteron Maruca vitrata

Cavaliere

Cara²,

Polito³

et al. 2009

Int. J. Dev. Biol.

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“…mst36Fa and mst36Fb are germline male‐specific genes, the transcripts of which appear in the primary spermatocytes and persist until early postmeiotic stages of spermatogenesis (Di Cara et al ., 2006).…”

Section: Resultsmentioning

confidence: 99%

“…We previously showed that −500 to +800 and −145 to +10 bp around the mst36Fa and mst36Fb transcription start points, respectively, are sufficient for correct cell‐specific transcription of both reporter gene constructs (Di Cara et al ., 2006). Here we further investigate the size of the mst36Fa and mst36Fb promoters.…”

Section: Resultsmentioning

confidence: 99%

“…In a previous work, we showed that a sequence of 800 bp downstream of the mst36Fa transcription start site is able to confer a delay of translation until the postmeiotic stages of the mst36Fa‐ lacZ gene construct (Di Cara et al ., 2006). After reviewing the literature data that indicate that in testes translational control regions are in the 5′ untranslated region (5′UTR; Schäfer et al ., 1990; Blumer et al ., 2002; Renkawitz‐Pohl et al ., 2005; Hempel et al ., 2006), we wanted to see whether this could be the case for mst36Fa and mst36Fb transcripts.…”

Section: Resultsmentioning

confidence: 99%

“…The two transcripts of the cluster, mt36Fa and mst36Fb , are exclusively accumulated in the male germline during spermatogenesis. Although they are strictly clustered and show an identical expression profile, in vivo analysis showed that their expression is triggered by distinct promoter sequences (Di Cara et al ., 2006).…”

Section: Introductionmentioning

confidence: 99%

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Expressional and functional analysis of the male‐specific cluster mst36F during Drosophila spermatogenesis

Cara

Cavaliere

Galliero

et al. 2010

Insect Molecular Biology

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mst36Fa and mst36Fb are two male-specific genes that are part of a novel gene family recently characterized in Drosophila melanogaster. The genes are strictly clustered and show an identical tissue and temporal expression pattern limited to the male germline. Here we demonstrate that the transcription of these two genes, which is triggered by different cis regulatory elements, responds to the same testisspecific factors encoded by the aly and can class meiotic arrest genes. RNA interference was used to decrease expression of these two genes. We obtained a reduction of fertility in the transgenic adult males compared to the wild type. These data suggest that the Mst36Fa and Mst36Fb proteins may have an important role in the production of functional sperm.

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Molecular technologies to improve the effectiveness of the sterile insect technique

Franz

Robinson

2011

Genetica

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The application of the Sterile Insect Technique (SIT) in area-wide integrated pest management (AW-IPM) programmes continues to increase. However, programme efficiency can still be considerably enhanced when certain components of the technology are improved, such as the development of improved strains for mass rearing and release. These include strains that (1) produce only male insects for sterilization and release and (2) carry easily identifiable markers to identify released sterile insects in the field. Using both classical and modern biotechnology techniques, key insect pests are targeted, where SIT programmes are being implemented. The pests include mosquitoes, the Mexican fruit fly, the codling moth, the oriental fruit fly and the pink bollworm. This special issue summarizes the results of research efforts aimed at the development and evaluation of new strains to a level where a decision can be made as to their suitability for use in large scale SIT programmes. Major beneficiaries will be operational AW-IPM programmes that apply the SIT against major insect pests.

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Structure and expression of a novel gene family showing male germline specific expression in Drosophila melanogaster

Cited by 10 publications

References 33 publications

Cloning and functional characterization of the intersex homologous gene in the pest lepidopteron Maruca vitrata

Cloning and functional characterization of the intersex homologous gene in the pest lepidopteron Maruca vitrata

Expressional and functional analysis of the male‐specific cluster mst36F during Drosophila spermatogenesis

Molecular technologies to improve the effectiveness of the sterile insect technique

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