Structure and Cooperativity of the Cytosolic Domain of the CorA Mg2+ Channel from Escherichia coli

Lerche, M.; Sandhu, Hena; Flöckner, Lukas; Högbom, Martin; Rapp, Mikaela

doi:10.1016/j.str.2017.05.024

Cited by 14 publications

(11 citation statements)

References 51 publications

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“…The transport mechanisms of loading and unloading in vascular tissues, in particular, remain unknown 16 . In prokaryotes, the so-called CorA channel, and in eukaryotes, its homologue Mitochondrial RNA Splicing 2 (MRS2), are the major Mg 2+ uptake systems 18 . The best-studied Mg transport family identified in higher plants belongs to homologues of the CorA Mg transporters and are called MGTs, standing for magnesium transporters.…”

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confidence: 99%

Root growth in light of changing magnesium distribution and transport between source and sink tissues in potato (Solanum tuberosum L.)

Koch

Winkelmann

Hasler

et al. 2020

Sci Rep

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This study depicts relations between magnesium (Mg) transport and re-translocation, photoassimilate partitioning, cation and ion concentrations, and finally root growth of potato under different Mg supplies. Potato plants were grown in a hydroponic culture system under different Mg regimes while investigating Mg concentrations, the expression of various Mg transporters, soluble sugars, and cations and anions in source and sink organs at different growth stages. Reports from literature about the impact of Mg deficiency on root growth are inconsistent. As Mg is known to be a phloem mobile nutrient, it is expected to be re-translocated under restricted availability of Mg from source to sink organs. Thus, we assume that plants can tolerate a slight Mg restriction without severe root growth reduction. However, under severe Mg deficiency, the process of Mg re-translocation is hampered, resulting in an impaired photoassimilate partitioning, and finally root growth. This might also explain the findings of studies claiming that Mg deficiency does not impair root growth as plants of these studies likely only suffered a slight Mg restriction. Finally, this study gives indications that an interruption of the process of Mg-re-translocation in early plant growth could be an indicator for growth reductions of the plant at a later growth stage.

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confidence: 99%

Root growth in light of changing magnesium distribution and transport between source and sink tissues in potato (Solanum tuberosum L.)

Koch

Winkelmann

Hasler

et al. 2020

Sci Rep

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“…3A). Structural analysis of CorA has shown that the protein is a pentamer, with each subunit contributing two transmembrane (TM) helices and containing a bulky cytosolic part (74,75). The stable association of CorA with type III-B CRISPR-Cas strongly suggests a functional connection, and more specifically, a link between the CRISPRmediated defense and membrane processes.…”

Section: Crispricity Pipelinementioning

confidence: 99%

Systematic prediction of genes functionally linked to CRISPR-Cas systems by gene neighborhood analysis

Shmakov

Makarova

Wolf

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

145

147

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The CRISPR-Cas systems of bacterial and archaeal adaptive immunity consist of direct repeat arrays separated by unique spacers and multiple CRISPR-associated () genes encoding proteins that mediate all stages of the CRISPR response. In addition to the relatively small set of core genes that are typically present in all CRISPR-Cas systems of a given (sub)type and are essential for the defense function, numerous genes occur in CRISPR- loci only sporadically. Some of these have been shown to perform various ancillary roles in CRISPR response, but the functional relevance of most remains unknown. We developed a computational strategy for systematically detecting genes that are likely to be functionally linked to CRISPR-Cas. The approach is based on a "CRISPRicity" metric that measures the strength of CRISPR association for all protein-coding genes from sequenced bacterial and archaeal genomes. Uncharacterized genes with CRISPRicity values comparable to those of genes are considered candidate CRISPR-linked genes. We describe additional criteria to predict functionally relevance for genes in the candidate set and identify 79 genes as strong candidates for functional association with CRISPR-Cas systems. A substantial majority of these CRISPR-linked genes reside in type III CRISPR- loci, which implies exceptional functional versatility of type III systems. Numerous candidate CRISPR-linked genes encode integral membrane proteins suggestive of tight membrane association of CRISPR-Cas systems, whereas many others encode proteins implicated in various signal transduction pathways. These predictions provide ample material for improving annotation of CRISPR- loci and experimental characterization of previously unsuspected aspects of CRISPR-Cas system functionality.

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“…The crystal structure of CorA from Thermotoga maritima in its Mg 2+ -bound closed state (Eshaghi et al, 2006; Lunin et al, 2006; Payandeh and Pai, 2006) revealed a 5-fold symmetric homo-pentamer forming an ~11 nm funnel-like structure, with large intracellular domains and Mg 2+ -ions bound between the subunits (Eshaghi et al, 2006; Guskov et al, 2012; Lerche et al, 2017; Lunin et al, 2006; Payandeh and Pai, 2006). Through CorA, Mg 2+ -homeostasis is achieved by a negative feedback mechanism in which Mg 2+ acts as both, charge carrier and ligand (Dalmas et al, 2014b).…”

Section: Introductionmentioning

confidence: 99%

Real time dynamics of Gating-Related conformational changes in CorA

Rangl

Schmandt

Perozo

et al. 2019

eLife

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CorA, a divalent-selective channel in the metal ion transport superfamily, is the major Mg2+-influx pathway in prokaryotes. CorA structures in closed (Mg2+-bound), and open (Mg2+-free) states, together with functional data showed that Mg2+-influx inhibits further Mg2+-uptake completing a regulatory feedback loop. While the closed state structure is a symmetric pentamer, the open state displayed unexpected asymmetric architectures. Using high-speed atomic force microscopy (HS-AFM), we explored the Mg2+-dependent gating transition of single CorA channels: HS-AFM movies during Mg2+-depletion experiments revealed the channel’s transition from a stable Mg2+-bound state over a highly mobile and dynamic state with fluctuating subunits to asymmetric structures with varying degree of protrusion heights from the membrane. Our data shows that at Mg2+-concentration below Kd, CorA adopts a dynamic (putatively open) state of multiple conformations that imply structural rearrangements through hinge-bending in TM1. We discuss how these structural dynamics define the functional behavior of this ligand-dependent channel.

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Structure and Cooperativity of the Cytosolic Domain of the CorA Mg2+ Channel from Escherichia coli

Cited by 14 publications

References 51 publications

Root growth in light of changing magnesium distribution and transport between source and sink tissues in potato (Solanum tuberosum L.)

Root growth in light of changing magnesium distribution and transport between source and sink tissues in potato (Solanum tuberosum L.)

Systematic prediction of genes functionally linked to CRISPR-Cas systems by gene neighborhood analysis

Real time dynamics of Gating-Related conformational changes in CorA

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