Structure and conformation of acetan polysaccharide

Ojinnaka, Cordelia; Jay, A.J.; Colquhoun, Ian J.; Brownsey, G.J.; Morris, Edwin R.; Morris, V. J.

doi:10.1016/0141-8130(96)01120-8

Cited by 30 publications

(16 citation statements)

References 21 publications

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“…One of the most studied polysaccharides is a xanthan gum-like EPS, named ‘acetan’, the structure of which is shown in Supplementary Information. The chemical repeat unit consists of a cellobiose unit solubilized by attachment of a charged pentasaccharide sidechain to one of the glucose residues 33, 34 . Genetic analysis of RZS01 shows that the biosynthesis of acetan occurs via a pathway similar to that for BC biosynthesis.…”

Section: Resultsmentioning

confidence: 99%

Complete genome sequence of the cellulose-producing strain Komagataeibacter nataicola RZS01

Zhang

Xiao

et al. 2017

Sci Rep

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Komagataeibacter nataicola is an acetic acid bacterium (AAB) that can produce abundant bacterial cellulose and tolerate high concentrations of acetic acid. To globally understand its fermentation characteristics, we present a high-quality complete genome sequence of K. nataicola RZS01. The genome consists of a 3,485,191-bp chromosome and 6 plasmids, which encode 3,514 proteins and bear three cellulose synthase operons. Phylogenetic analysis at the genome level provides convincing evidence of the evolutionary position of K. nataicola with respect to related taxa. Genomic comparisons with other AAB revealed that RZS01 shares 36.1%~75.1% of sequence similarity with other AAB. The sequence data was also used for metabolic analysis of biotechnological substrates. Analysis of the resistance to acetic acid at the genomic level indicated a synergistic mechanism responsible for acetic acid tolerance. The genomic data provide a viable platform that can be used to understand and manipulate the phenotype of K. nataicola RZS01 to further improve bacterial cellulose production.

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Section: Resultsmentioning

confidence: 99%

Complete genome sequence of the cellulose-producing strain Komagataeibacter nataicola RZS01

Zhang

Xiao

et al. 2017

Sci Rep

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“…50 Morris suggested that there was a possibility that pyruvate group in xanthan has a crucial role in the synergistic interaction with galactomannan and/or glucomannan but that pyruvate groups were not always necessary based on the results of the synergistic interaction between acetan, a bacterial anionic polysaccharide with a similar structure to xanthan, having a longer side chain, pentasaccharide instead of the trisaccharide and having no pyruvic substituent. 35,51,52 However, there is still a possibility that molecular interaction mechanisms are different for xanthan/galactomannan and acetan/galactomannan, and it was reported that a pronounced thermal hysteresis was observed on heating−cooling cycle for the mixture of deacetylated acetan and LBG, which was not seen for xanthan/galactomannan. 51,52 Constructing molecular structure model of the intermolecular association in liquid is not possible only based on NOE data at this stage due to spin diffusion.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…35,51,52 However, there is still a possibility that molecular interaction mechanisms are different for xanthan/galactomannan and acetan/galactomannan, and it was reported that a pronounced thermal hysteresis was observed on heating−cooling cycle for the mixture of deacetylated acetan and LBG, which was not seen for xanthan/galactomannan. 51,52 Constructing molecular structure model of the intermolecular association in liquid is not possible only based on NOE data at this stage due to spin diffusion. Therefore, the role of the pyruvate groups had not been clear.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Solution Structure of Molecular Associations Investigated Using NMR for Polysaccharides: Xanthan/Galactomannan Mixtures

Takemasa

Nishinari

2016

J. Phys. Chem. B

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Although the intermolecular nuclear Overhauser effect (NOE) signal was valuable to elucidate molecular association structure, it could not always be observed for associated molecules due to the short spin-spin relaxation time T2 in NMR measurements, especially for high molar mass systems. While almost no study has been reported for high molar mass polymers (>1 × 10(6)), especially for polysaccharide-polysaccharide interactions, NOE signals were observed for the first time between two different types of polysaccharides, xanthan and galactomannan (locust bean gum), forming a synergistic gel, as a direct evidence of intermolecular binding of polysaccharides. The NOE peak was found between pyruvic acid in xanthan and anomeric proton of mannose of galactomannan. This NOE signal was observed only when mixing time >0.5 s, indicating indirect NOEs caused by spin diffusion. Therefore, this NOE could not be used to construct the molecular models. However, it is a direct evidence for the binding between two different types of polysaccharide to elucidate the synergistic gelation. This NOE signal was observed only for low molar mass galactomannans (1.4 × 10(4)). T2 of pyruvate methyl drastically decreased at low temperatures in the presence of synergistic interaction, suggesting that pyruvate group at terminal end of side chain in xanthan plays an essential role in synergistic interaction.

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“…27 It is structurally similar to xanthan (Figure 1) but contains a pentasaccharide side chain. [27][28][29][30] Both xanthan and acetan contain noncarbohydrate substituents. 23,29,30 In both cases, the (1 f 2) D-mannose residue is partially O-acetylated.…”

Section: Introductionmentioning

confidence: 99%

“…[27][28][29][30] Both xanthan and acetan contain noncarbohydrate substituents. 23,29,30 In both cases, the (1 f 2) D-mannose residue is partially O-acetylated. Acetan shows no equivalent to the acetyl or pyruvate substitution of xanthan on the terminal mannose residue.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Interactions of Acetan with Carob or Konjac Mannan

1998

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New experimental evidence has been obtained which demonstrates synergistic interaction of mixtures of acetan with carob or konjac mannan. These binary mixtures have been shown to form thermoreversible gels under conditions for which the individual components do not gel. The rheological properties of acetan-carob and acetan-konjac mannan mixtures have been studied at various concentrations above and below the threshold concentration for gelation. In dilute solutions, the synergisms manifested themselves as an enhancement of the viscosity, whereas at higher concentrations gelation occurred. The synergistic effects were much weaker for acetan-konjac mannan mixtures than for acetancarob mixtures. Deacetylation of acetan enhanced the synergistic interactions, particularly for acetankonjac mannan mixtures, and also altered the polymer ratio at which the maximum interactions were observed. The influence of the stability of the acetan helix on the gelation of the binary mixtures was investigated by varying the mixing (preparation) temperature and the level of salt added to the mixtures. These studies suggest that factors destabilizing the acetan helix favor gelation.

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Structure and conformation of acetan polysaccharide

Cited by 30 publications

References 21 publications

Complete genome sequence of the cellulose-producing strain Komagataeibacter nataicola RZS01

Complete genome sequence of the cellulose-producing strain Komagataeibacter nataicola RZS01

Solution Structure of Molecular Associations Investigated Using NMR for Polysaccharides: Xanthan/Galactomannan Mixtures

Synergistic Interactions of Acetan with Carob or Konjac Mannan

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