Structure and biological activity of polysaccharides from Nitraria sibirica pall fruit

Abuduwaili, Aytursun; Mutailifu, Paiheerding; Nuerxiati, Rehebati; Gao, Yanhua; Aisa, Haji Akber; Yili, Abulimiti

doi:10.1016/j.fbio.2021.100903

Cited by 15 publications

(12 citation statements)

References 32 publications

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“…The sodium periodate oxidation results showed that 1 M glycosyl consumed 0.7491 mol of NaIO4 and liberated 0.007 mol of formic acid. The consumption of NaIO4 was more than double that of HCOOH, indicating the possible existence of 1→2, 1→2,6, 1→4, or 1→4,6, as well as 1→ or 1→6 linked glycosidic bonds ( 34 ). Furthermore, the GC analysis of the products of Smith degradation showed that it contained glycerin, further indicating that monosaccharides of HEP-4 could be connected largely by 1→2 or 1→2,6, and 1→ or 1→6 linked glycosidic bonds ( 34 ).…”

Section: Resultsmentioning

confidence: 99%

“…The consumption of NaIO4 was more than double that of HCOOH, indicating the possible existence of 1→2, 1→2,6, 1→4, or 1→4,6, as well as 1→ or 1→6 linked glycosidic bonds ( 34 ). Furthermore, the GC analysis of the products of Smith degradation showed that it contained glycerin, further indicating that monosaccharides of HEP-4 could be connected largely by 1→2 or 1→2,6, and 1→ or 1→6 linked glycosidic bonds ( 34 ). Additionally, the presence of galactose was indicative of HEP-4 with the existence of 1→3, 1→3,4, and 1→3,6, linkages that cannot be oxidized ( 24 ).…”

Section: Resultsmentioning

confidence: 99%

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Extraction, purification, structural characterization, and antioxidant activity of a novel polysaccharide from Lonicera japonica Thunb.

Ren²,

Wu³

et al. 2022

Front. Nutr.

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A novel water-soluble polysaccharide (HEP-4) with a molecular weight of 1.98 × 105Da was extracted from honeysuckle. Structural characterization was performed using high-performance liquid chromatography (HPLC), gas chromatography, Fourier transform-infrared (FT-IR) spectrum, nucleus magnetic resonance (NMR) spectra, and scanning electron microscopy. The results showed that HEP-4 is primarily composed of mannose, rhamnose, galacturonic acid, glucose, galactose, and arabinose with a mole ratio of 6.74:1.56:1.04:14.21:4.31:5.4, and the major types of the glycosidic bond types of HEP-4 were 1-α-D-Glcp, 1,4-β-D-Glcp, 1-β-D-Arap, 1,3,4-β-D-Arap, and 1,3,6-β-D-Manp. The results of bioactivity experiments revealed that HEP-4 had antioxidant in vitro. In addition, HEP-4 inhibited H2O2-induced oxidative damage and increased the activity of HepG2 cells by reducing MDA levels and inhibiting ROS production. Meanwhile, HEP-4 significantly enhanced the activities of GSH-Px and CAT, indicating that HEP-4 exerts a protective effect on H2O2-induced oxidative stress. These results indicate that HEP-4 could be a potential natural antioxidant.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Extraction, purification, structural characterization, and antioxidant activity of a novel polysaccharide from Lonicera japonica Thunb.

Ren²,

Wu³

et al. 2022

Front. Nutr.

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“…In total, 40 mg of polysaccharide was dissolved in 1 mL of 99.9% deuterium oxide and the sample was placed in NMR tubes. The 1 H NMR and 13 C NMR spectra were recorded on an NMR spectrometer (Varian VNMRS 600 spectrometer, Palo Alto, CA, USA) [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

“…The DPPH and ABTS free radicals scavenging activity of the polysaccharides were conducted according to our reported method [ 38 ]. DPPH test described briefly: 1 mL of different concentrations of polysaccharides in water (0.25–2.0 mg/mL) was mixed with 1 mL of freshly prepared DPPH in methanol (0.2 mmol/L).…”

Section: Methodsmentioning

confidence: 99%

Isolation, Structural Characterization, and Biological Activity of the Two Acidic Polysaccharides from the Fruits of the Elaeagnus angustifolia Linnaeus

et al. 2022

Self Cite

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Elaeagnus angustifolia Linnaeus is a medicinal plant and its fruit has pharmacological activity such as antiinflammatory, antiedema, antinociceptive, and muscle relaxant functions, etc. Two acidic homogeneous polysaccharides (EAP-H-a1 and EAP-H-a2) were isolated from the fruits of Elaeagnus angustifolia L. through DEAE-52 and Sephadex G-75 column chromatography, and the physicochemical, structural properties, and biological activities of the polysaccharides were investigated. Both EAP-H-a1 and EAP-H-a2 were composed of Rha, Ara, Xyl, Glc, and Gal with the molar ratios of 13.7:20.5:23.3:8.8:33.4 and 24.8:19.7:8.2:8.4:38.6, respectively, and with the molecular weights of 705.796 kDa and 439.852 kDa, respectively. The results obtained from Fourier transform infrared spectroscopy (FTIR) confirmed the polysaccharide nature of the isolated substances. Congo red assay confirmed the existence of a triple-helix structure. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis revealed that EAP-H-a1 and EAP-H-a2 had irregular fibrous, filament-like surfaces; and both had crystalline and amorphous structures. Bioactivity analysis showed that the crude polysaccharide, EAP-H-a1, and EAP-H-a2 had clear DPPH and ABTS free radical scavenging activity, and could promote the secretion of NO and the phagocytic activities of RAW 264.7 and THP cells, which showed clear antioxidant and immuno-regulatory activity. These results indicated that Elaeagnus angustifolia L. fruit acidic polysaccharides may have potential value in the pharmaceutical and functional food industries.

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“…Water extract–alcohol precipitation is mostly used in the extraction of NSP (Ketha & Gudipati, 2018), and microwave‐assisted, enzyme‐assisted, and ultrasonic‐assisted extraction are also used to extract non‐starch polysaccharide. (Guo et al, 2019; Jiao et al, 2017) Recently, many researchers have directed their attention to polysaccharides because of their biological functions, including antioxidation (Gong et al, 2021; Yang et al, 2021), immunoregulation (Abuduwaili et al, 2021), hypoglycemic effects (Li et al, 2021; Xiao et al, 2018; Zhong et al, 2020; Zhou et al, 2020), and anti‐inflammatory effects (Jin et al, 2020). Polysaccharides can be extracted from grain resources.…”

Section: Introductionmentioning

confidence: 99%

Structural characterization of a non‐starch polysaccharide from sweet corn cobs

Wang

Xiu

Han

et al. 2022

Food Processing Preservation

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In order to improve the utilization value of sweet corn cobs, we extracted a new nonstarch polysaccharide from sweet corn cobs and named it SCP-80-I. It showed that SCP-80-I was a neutral homogeneous polysaccharide composed of glucose with a molecular weight of 175.453 kDa. We also explore the structural features of SCP-80-I and we found that it possessed a backbone of →4-Glc-1 → 4-Glc-1 → 4,6-Glc-1 → 4-Glc-1→, and the branched chain was →6-Glc-1 → linked to the main chain through O-6 of →4,6-Glc-1→. We predicted that SCP-80-I may have a function of antioxidation and hypoglycemic due to its structure. This study provides some reference value for sweet corn cob polysaccharides in the creation of functional food. Novelty impact statement:1. A new non-starch polysaccharide from sweet corn cobs (SCP-80-I) was obtained.2. SCP-80-I was composed of glucose with a molecular weight of 175.453 kDa.

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Structure and biological activity of polysaccharides from Nitraria sibirica pall fruit

Cited by 15 publications

References 32 publications

Extraction, purification, structural characterization, and antioxidant activity of a novel polysaccharide from Lonicera japonica Thunb.

Extraction, purification, structural characterization, and antioxidant activity of a novel polysaccharide from Lonicera japonica Thunb.

Isolation, Structural Characterization, and Biological Activity of the Two Acidic Polysaccharides from the Fruits of the Elaeagnus angustifolia Linnaeus

Structural characterization of a non‐starch polysaccharide from sweet corn cobs

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