Structure and Bioactivities of a Novel Polysaccharide Extracted From Dendrobium huoshanense by Subcritical Water

Abstract

In this study, the polysaccharide was extracted by subcritical water from Dendrobium huoshanense. A novel polysaccharide (DHPs-1) was obtained through several purification steps and its structure and bioactivity were investigated. Structural analysis indicated that the weight-average molecular weight of DHPs-1 was 5.0 × 104 Da and it was mainly composed of glucose (65.04%), mannose (14.23%), galactose (8.17%), galacturonic acid (6.41%), rhamnose (2.34%), and xylose (1.25%). 1,4-Glcp, and 1,4,6-Galp were existed in the backbone of DHPs-1. The residues of 1,3,4-Galp, 1,4-Manp, 1,4-Galp, and 1,3,4,6-Galp could be in the backbone or the side chains with the non-reducing terminal of α-Manp. Bioactivity tests indicated that DHPs-1 had immunomodulatory activity in that it significantly enhanced transcript levels of cytokines [Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-1β (IL-1β), and Interleukin-10 (IL-10)]. DPPH and hydroxyl radical scavenging tests showed that it had good antioxidant activity. These results reveal that DHPs-1 could be developed as a safe immunomodulatory agent and antioxidant for pharmacological or functional food applications.

Quantitative Identification of Antioxidant Basis for Dendrobium Nobile Flower by High Performance Liquid Chromatography-Tandem Mass Spectrometry

“…Some flavonoids, such as quercetin, rutin, and isoquercitrin, had been reported to be correlated antioxidant activities in D. officinale, D. catenatum, and D. huoshanense [12,[19][20][21]. Some polysaccharides were also considered as functional antioxidants in D. officinale, D. huoshanense, and D. nobile [15,22,23]. Recently, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/ MS) had been used for metabolic analysis, chemical differentiation, quality control, and pharmaceutical identification in some Dendrobium species [19,20,24].…”

Preparation of nano-selenium from chestnut polysaccharide and characterization of its antioxidant activity

“…The chemical bonds and functional groups of chestnut polysaccharides CP and CP-1a were determined by FT-IR technology ( 51 , 52 ). Figure 8 shows that the absorption peak between 950 and 1,160 cm −1 is generated by the tensile vibration of pyranose ring ( 53 – 57 ).…”

Extraction and Purification, Structural Characterization and Biological Activity of a Polysaccharide, PRP‐1, from Plumeria rubra

“…SEM was a very useful tool to study surface structure of polysaccharides [26] . The surface morphology of PRP‐1 at 100, 200, 500, and 1000 times were observed by SEM (Figure 6.A–6.D).…”

“…SEM was a very useful tool to study surface structure of polysaccharides. [26] The surface morphology of PRP-1 at 100, 200, 500, and 1000 times were observed by SEM (Figure 6.A-6.D). The images revealed PRP-1 has a compact three-dimensional curling network structure with the surface was smooth.…”