Structure and associated mutational effects of the cysteine proteinase (CP1) gene of <i>Drosophila melanogaster</i>

Gray, Yasmine H. M.; Sved, J. A.; Preston, Christine R.; Engels, William R.

doi:10.1046/j.1365-2583.1998.00070.x

Cited by 19 publications

(9 citation statements)

References 8 publications

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“…In some cases NHEJ was inferred from a PCR fragment larger or smaller than that expected from templated repair. In other cases, the mutant phenotype of the CP1 gene (Gray et al 1998) implied loss of sequences flanking the insertion, as would be consistent with end joining. For example, the 4-kb deletion yielded 91 offspring of category A9 (Table 1).…”

Section: Discussionmentioning

confidence: 64%

The Effect of Gap Length on Double-Strand Break Repair in Drosophila

Johnson-Schlitz

Engels

2006

Genetics

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When a double-strand break has a gap between the broken ends, the missing information can be restored through synthesis from a homologous template. Here we address the question of how long such a gap can be before this process fails. We measured the frequency of homologous repair in the Drosophila germ line following the creation of gaps of specific sizes ranging from 3.8 to 210 kb. We found that gaps of #11 kb can be repaired with approximately the same efficiency as breaks with no gap at all. However, a gap of 44 kb was repaired only rarely, and one of 210 kb was not repaired at a measurable frequency. We conclude that DNA gap repair is a length-limited process, but that this limitation is critical only for gaps ?11 kb.

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Section: Discussionmentioning

confidence: 64%

The Effect of Gap Length on Double-Strand Break Repair in Drosophila

Johnson-Schlitz

Engels

2006

Genetics

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“…b encodes a product involved in b-alanine biosynthesis; b mutants are heavily pigmented. Cp1 encodes a product with cathepsin L activity; deletion studies of Cp1 have shown complete female sterility and reduced pigmentation in abdominal segments 1-5 (Gray et al 1998). Bc encodes a tyrosinase, which catalyzes the de novo synthesis of melanin from tyrosine (Wittkopp et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Quantitative Trait Loci Affecting the Difference in Pigmentation BetweenDrosophila yakubaandD. santomea

et al. 2005

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Using quantitative trait locus (QTL) mapping, we studied the genetic basis of the difference in pigmentation between two sister species of Drosophila: Drosophila yakuba, which, like other members of the D. melanogaster subgroup, shows heavy black pigmentation on the abdomen of males and females, and D. santomea, an endemic to the African island of São Tomé, which has virtually no pigmentation. Here we mapped four QTL with large effects on this interspecific difference in pigmentation: two on the X chromosome and one each on the second and third chromosomes. The same four QTL were detected in male hybrids in the backcrosses to both D. santomea and D. yakuba and in the female D. yakuba backcross hybrids. All four QTL exhibited strong epistatic interactions in male backcross hybrids, but only one pair of QTL interacted in females from the backcross to D. yabuka. All QTL from each species affected pigmentation in the same direction, consistent with adaptive evolution driven by directional natural selection. The regions delimited by the QTL included many positional candidate loci in the pigmentation pathway, including genes affecting catecholamine biosynthesis, melanization of the cuticle, and many additional pleiotropic effects.

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“…The first introns of the cow and dog orthologs also are located 11 nucleotides upstream of the ATG initiation codon [59,60]. An intron also is present in the 5 0 -UTR of the Drosophila melanogaster ortholog [61], which is located 13 nucleotides upstream of the translation start site. Since the location of the Ctsl first intron within the 5 0 -UTR is highly conserved in several species, this common feature invites the speculation that the Ctsl first intron plays a general role in the upregulation of this gene.…”

Section: Location Of the Ctsl First Intron Is Conserved In Several Spmentioning

confidence: 99%

The Cathepsin L First Intron Stimulates Gene Expression in Rat Sertoli Cells1

Charron

Chern

Wright

2007

Biology of Reproduction

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Large amounts of cathepsin L (CTSL), a cysteine protease required for quantitatively normal spermatogenesis, are synthesized by mouse and rat Sertoli cells during stages VI to VII of the cycle of the seminiferous epithelium. We previously demonstrated that all of the regulatory elements required in vivo for both Sertoli cell- and stage-specific expression of the Ctsl gene are present within a ~3-kb genomic fragment that contains 2065 nucleotides upstream of the transcription start site and 977 nucleotides of downstream sequence. Most of the downstream region encodes the first intron. In this study, transient transfection assays using primary Sertoli cell cultures and the TM4 Sertoli cell line established that the Ctsl first intron increased reporter gene activity by ~5-fold. While the intron-mediated enhancement in reporter gene activity was not restricted to the Ctsl promoter, positioning the first intron upstream of the Ctsl promoter in either orientation abolished its stimulatory activity, suggesting that it does not contain a typical enhancer. Mutating the 5'-splice site of the Ctsl first intron or replacing the first intron by the Ctsl fourth intron abolished the stimulatory effect. Finally, the intron-dependent increase in reporter gene activity could be explained in part by an increase in the amounts of total RNA and transcript polyadenylation. Results from this study suggest that the stimulatory effect mediated by the Ctsl first intron may explain in part why Sertoli cells in seminiferous tubules at stages VI to VII produce high levels of CTSL.

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Structure and associated mutational effects of the cysteine proteinase (CP1) gene of Drosophila melanogaster

Cited by 19 publications

References 8 publications

The Effect of Gap Length on Double-Strand Break Repair in Drosophila

The Effect of Gap Length on Double-Strand Break Repair in Drosophila

Quantitative Trait Loci Affecting the Difference in Pigmentation BetweenDrosophila yakubaandD. santomea

The Cathepsin L First Intron Stimulates Gene Expression in Rat Sertoli Cells1

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