Structure and activity of lipid membrane biosensor surfaces studied with atomic force microscopy and a resonant mirror

Fisher, M.; Tjärnhage, Torbjörn

doi:10.1016/s0956-5663(00)00105-6

Cited by 46 publications

(24 citation statements)

References 22 publications

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“…In situations where high affinity antibodies are not available or when detection of classes of toxins is desired, nonantibody-based assays have been utilized in fieldable biosensors [41,42,43,44,45,46,47,48,49,50,51,52,53,54]. Biosensor-based detection of neurotoxins has been accomplished using the enzyme acetylcholinesterase [42,47,49,53,54], acetylcholine receptors [41,43], and networks of cultured neural cells [52].…”

Section: Detection Of Toxins Using Cellular Receptorsmentioning

confidence: 99%

“…Biosensor-based detection of neurotoxins has been accomplished using the enzyme acetylcholinesterase [42,47,49,53,54], acetylcholine receptors [41,43], and networks of cultured neural cells [52]. Ganglioside receptors have been used in biosensors to detect binding of toxins such as cholera toxin [44,46,48,51,55,56], Escherichia coli heat-labile enterotoxin [44,49], tetanus toxin [50], and botulinum toxin [44,50].…”

Section: Detection Of Toxins Using Cellular Receptorsmentioning

confidence: 99%

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Array biosensor for detection of toxins

Ligler

Taitt

Shriver‐Lake

et al. 2003

Analytical and Bioanalytical Chemistry

270

133

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The array biosensor is capable of detecting multiple targets rapidly and simultaneously on the surface of a single waveguide. Sandwich and competitive fluoroimmunoassays have been developed to detect high and low molecular weight toxins, respectively, in complex samples. Recognition molecules (usually antibodies) were first immobilized in specific locations on the waveguide and the resultant patterned array was used to interrogate up to 12 different samples for the presence of multiple different analytes. Upon binding of a fluorescent analyte or fluorescent immunocomplex, the pattern of fluorescent spots was detected using a CCD camera. Automated image analysis was used to determine a mean fluorescence value for each assay spot and to subtract the local background signal. The location of the spot and its mean fluorescence value were used to determine the toxin identity and concentration. Toxins were measured in clinical fluids, environmental samples and foods, with minimal sample preparation. Results are shown for rapid analyses of staphylococcal enterotoxin B, ricin, cholera toxin, botulinum toxoids, trinitrotoluene, and the mycotoxin fumonisin. Toxins were detected at levels as low as 0.5 ng mL(-1).

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Section: Detection Of Toxins Using Cellular Receptorsmentioning

confidence: 99%

Section: Detection Of Toxins Using Cellular Receptorsmentioning

confidence: 99%

Array biosensor for detection of toxins

Ligler

Taitt

Shriver‐Lake

et al. 2003

Analytical and Bioanalytical Chemistry

270

133

View full text Add to dashboard Cite

show abstract

“…Studies describing the immobilization and use of different kinds of recognition molecules, such as receptors, in planar waveguide TIRF systems are limited to a handful of papers (82,90,91]. A number of receptors have shown promise for use in fiber optic, SPR and resonant mirror sensors [ [124][125][126][127][128]. The methods used in these studies may also prove successful for the immobilization of functional receptors to planar waveguides for use in TIRF and interferometric biosensors.…”

Section: The Futurementioning

confidence: 99%

TIRF Array Biosensor for Environmental Monitoring

Sapsford¹,

Ligler²

2004

Springer Series on Chemical Sensors and Biosensors

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“…Based on the sensing surface constructed by biological membrane-like lipid films with incorporated GM1 analogues, a reagentless optical biosensor was developed for rapid assay of protein toxins using the resonant mirror in-strument and SPR (surface plasmon resonance). [6][7][8] Direct homogenous detection of CT was reported based on the color changes 9,10 and fluorescence quenching 11,12 or resonant energy transfer. 13,14 Liposomes, spherical vesicles composed of a phospholipid bilayer surrounding an aqueous cavity, were originally developed to study cell membranes.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Sensitive Piezoelectric Immunosensor for Cholera Toxin Based on GM1‐Incorporated Liposome Agglutination

Liu

Wang²,

et al. 2010

Chin. J. Chem.

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A sensitive piezoelelctric immunosensor has been developed for the direct detection of cholera toxin. The proposed technique is based on specific interface agglutination of GM1 incorporated liposome vesicles in the presence of the cholera toxin, which causes a frequency change and is monitored by a piezoelectric device. The interface agglutination reaction would take place as soon as the cholera toxin (CT) was captured by the antibodies modified on the probe surface and the GM1 incorporated liposome in the probe cell. Meanwhile, the agglutination reaction induced both the mass effect and viscoelastic effect acting on the probe surface. The results indicate that the probe signal can be observably multiplied. In addition, an optimization of liposome composition and assay medium were investigated. Moreover, no cross-reactivates were observed with the common protein, such as goat anti-mouse IgG (1 mg/mL), mouse IgG (1 mg/mL), goat anti-human IgG (1 mg/mL), and thrombin (1 mg/mL). The frequency responses of the developed piezoelelctric immunosensor are linearly correlated to cholera toxin concentration in the range of 0.5 to 7.5 µg/mL with a detection limit of 0.27 µg/mL while showed no significant loss in activity over 3 d under storage at 4 ℃.

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Structure and activity of lipid membrane biosensor surfaces studied with atomic force microscopy and a resonant mirror

Cited by 46 publications

References 22 publications

Array biosensor for detection of toxins

Array biosensor for detection of toxins

TIRF Array Biosensor for Environmental Monitoring

A Simple and Sensitive Piezoelectric Immunosensor for Cholera Toxin Based on GM1‐Incorporated Liposome Agglutination

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