Structure‐activity studies on the mutagenicity of tris(2,3‐dibromopropyl) phosphate (tris‐BP) and its metabolites in salmonella

The article contains sections titled: 1. Introduction 2. Phosphines 2.1. Properties 2.2. Production 2.2.1. Primary and Secondary Phosphines 2.2.2. Tertiary Phosphines 2.3. Uses 3. Halophosphines 3.1. Properties 3.2. Production 3.3. Uses 4. Phosphonium Salts 4.1. Properties 4.2. Production 4.3. Uses 5. Phosphine Oxides and Sulfides 5.1. Properties 5.2. Production 5.3. Uses 6. Phosphonous Acid Derivatives 6.1. Production 6.2. Properties and Uses 7. Phosphinic Acids and their Derivatives 7.1. Properties 7.2. Production 7.3. Phosphinic Acids and Phosphinate Esters 7.4. Thiophosphinates 7.5. Other Phosphinic Acid Derivatives 8. Phosphites and Hydrogenphosphonates 8.1. Properties 8.2. Production 8.3. Trialkyl Phosphites 8.4. Triaryl Phosphites and Alkyl Aryl Phosphites 8.5. Dialkyl and Diaryl Phosphonates 8.6. Alkyl Phosphonates 9. Phosphonic Acids and their Derivatives 9.1. Properties 9.2. Production 9.3. Phosphonic Acids and Phosphonocarboxylic Acids 9.4. Esters of Phosphonic Acid 9.5. Other Derivatives of Phosphonic Acid 10. Esters of Phosphoric Acid 10.1. Properties 10.2. Production 10.3. Trialkyl Phosphates 10.4. Triaryl and Alkyl Aryl Phosphates 10.5. Mono‐ and Dialkyl Phosphates 11. Esters of Thiophosphoric Acid 11.1. Properties 11.2. Production 11.3. Monothiophosphates 11.4. Dithiophosphates 12. Economic Aspects 13. Toxicology

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The stability of mutagenic chemicals stored in solution

Pagano

Zeiger

1985

Environ. mutagen

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Using the Salmonella/microsome assay, we have evaluated the stability of mutagenic responses of chemicals stored frozen over a period of 18 months. Each of the standard mutagens was prepared in January 1982, and aliquots were stored at -20 degrees C and at -80 degrees C. Sodium azide (NaN3) was dissolved in water; 4-nitro-o-phenylenediamine (4NOP), 4-nitroquinoline-N-oxide (4NQO), benzo(a)pyrene (B[a]P), and 2-aminoanthracene (2AA) were dissolved in DMSO, all at 100 micrograms/ml. At various times, aliquots were removed, thawed, and tested in parallel with freshly prepared mutagen samples using strain TA100 in a standard plate test and freshly prepared Aroclor 1254-induced rat liver S-9 mix where needed. 4NOP (2-10 micrograms/plate), 4NQO (0.01-0.10 micrograms/plate), B(a)P (0.5-2.5 micrograms/plate), and 2AA (0.25-2.0 micrograms/plate) showed no significant differences between the freshly prepared solutions and the solutions stored at -20 degrees C and -80 degrees C. NaN3 (0.1-0.8 micrograms/plate) did show a statistically significant difference, with the fresh samples giving the lowest mean responses (over all doses) and the -80 degrees C treatment giving the highest. The freezing of mutagen solutions is adaptable to routine use and provides the advantage of reducing the time required to prepare positive control chemicals and reducing the exposure of laboratory personnel to known mutagens.

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Structure‐activity studies on the mutagenicity of tris(2,3‐dibromopropyl) phosphate (tris‐BP) and its metabolites in salmonella

Cited by 6 publications

References 11 publications

Phosphorus Compounds, Organic

Phosphorus Compounds, Organic

Phosphorus Compounds, Organic

The stability of mutagenic chemicals stored in solution

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