Structure–activity relationships of Candida rugosa lipase immobilized on polylactic acid nanoparticles

Chronopoulou, Laura; Kamel, Gihan; Sparago, Carolina; Bordi, F.; Lupi, S.; Diociaiuti, Marco; Palocci, Cleofe

doi:10.1039/c0sm00712a

Cited by 59 publications

(31 citation statements)

References 35 publications

(37 reference statements)

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“…Protein adsorption on nanoparticles resulted in improved activity [14]. Enhanced thermal stability of enzymes was observed after adsorption.…”

Section: Page 7 Of 34mentioning

confidence: 98%

“…A c c e p t e d M a n u s c r i p t 14 Gel filtration chromatography gave 18.2% recovery of enzyme activity. SDS-PAGE analysis showed a single band, indicating complete purification of the enzyme.…”

Section: Identification Of Psychrophilic Strainsmentioning

confidence: 98%

“…Inactivation parameters comprising half-life (t 1/2 ), decay rate constant (k) and deactivation energy(E d ) were calculated according to Ortega et al (2004) [14]. The PGA concentration was 0.75% for this purpose.…”

Section: Thermal Stabilitymentioning

confidence: 99%

See 2 more Smart Citations

Nanotechnology based activation-immobilization of psychrophilic pectate lyase: A novel approach towards enzyme stabilization and enhanced activity

Mukhopadhyay

Bhattacharyya

Dasgupta

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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“…Protein adsorption on nanoparticles resulted in improved activity [14]. Enhanced thermal stability of enzymes was observed after adsorption.…”

Section: Page 7 Of 34mentioning

confidence: 98%

Section: Identification Of Psychrophilic Strainsmentioning

confidence: 98%

See 1 more Smart Citation

Nanotechnology based activation-immobilization of psychrophilic pectate lyase: A novel approach towards enzyme stabilization and enhanced activity

Mukhopadhyay

Bhattacharyya

Dasgupta

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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“…Moreover, in the spectrum of M-CRL2 the amide III band is partially overlapped with the amide II one consequently, to avoid errors in the baseline correction, these two bands were included in the fitting procedure. CRL conformation was determined by X-ray diffraction on lipase crystals [29,30]. XRD data revealed in the CRL open conformation a content 30% for α-helices and 12% for β-sheets.…”

Section: Resultsmentioning

confidence: 99%

Frozen Microemulsions for MAPLE Immobilization of Lipase

Califano

Bloisi

Perretta

et al. 2017

Preprint

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MAPLE (matrix assisted pulsed laser evaporation) depositions of Candida Rugosa lipase were carried out from ice matrices whose composition is optimized in order to minimize conformational damage of the protein, which strongly influences its catalytic activity. To induce lid opening and to protect lipase during the MAPLE process, pentane and m-DOPA amino acid were added to the liquid matrix giving a target formed by a frozen water-lipase-pentane microemulsion. FTIR and AFM were used to investigate the structure of MAPLE deposited lipase films. The ability of MAPLE films to promote transesterification was determined by thin layer chromatography. It was shown that m-DOPA has influence on the aggregation but not on the unfolding of lipase induced by MAPLE, while the microemulsion formed by the addition of pentane to the target composition is effective in protecting lipase during the MAPLE process. MAPLE deposited lipases showed a modified specificity.

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“…Thus, regeneration for reuse is often a difficult task and requires high-speed centrifugation for a long time. Non-magnetic nanoparticle carriers include zirconia, silica, polystyrene, chitosan, and polylactic acid (Table 1) [39,[47][48][49][50][51]. When immobilized on a hydrophilic inorganic surface, lipases have poor binding ability because a polypeptide chain "lid" of lipases secludes the catalytic site [52].…”

Section: Non-magnetic Nanoparticlesmentioning

confidence: 99%

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

Kim

Lee

2018

Catalysts

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Abstract:The cost of biodiesel production relies on feedstock cost. Edible oil is unfavorable as a biodiesel feedstock because of its expensive price. Thus, non-edible crop oil, waste oil, and microalgae oil have been considered as alternative resources. Non-edible crop oil and waste cooking oil are more suitable for enzymatic transesterification because they include a large amount of free fatty acids. Recently, enzymes have been integrated with nanomaterials as immobilization carriers. Nanomaterials can increase biocatalytic efficiency. The development of a nano-immobilized enzyme is one of the key factors for cost-effective biodiesel production. This paper presents the technology development of nanomaterials, including nanoparticles (magnetic and non-magnetic), carbon nanotubes, and nanofibers, and their application to the nano-immobilization of biocatalysts. The current status of biodiesel production using a variety of nano-immobilized lipase is also discussed.

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Structure–activity relationships of Candida rugosa lipase immobilized on polylactic acid nanoparticles

Cited by 59 publications

References 35 publications

Nanotechnology based activation-immobilization of psychrophilic pectate lyase: A novel approach towards enzyme stabilization and enhanced activity

Nanotechnology based activation-immobilization of psychrophilic pectate lyase: A novel approach towards enzyme stabilization and enhanced activity

Frozen Microemulsions for MAPLE Immobilization of Lipase

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

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