Amino acid surfactants have attracted attention as a naturally derived substrate for body cleansers and cosmetics. In this study, the antibacterial activity of lauroyl sarcosine, lauroyl glycine, and lauroyl alanine against Staphylococcus aureus and Staphylococcus epidermidis was evaluated. The minimum inhibitory concentrations of these surfactants were tens of μg mL -1 . These concentrations were smaller than most surfactants, indicating that amino acid surfactants are useful as antimicrobial agents against Staphylococci. In addition, they were less than the critical micelle concentration, suggesting that these surfactants act on the bacteria in a monomolecularly soluble state, rather than disrupting the bacterial membrane by solubilization. These findings suggest that amino acid surfactants are useful as antimicrobial agents in personal care products.