Structure-Activity Relationship of Synthetic Variants of the Milk-Derived Antimicrobial Peptide α _s2 -Casein f(183–207)

Milk bioactive peptide database: A comprehensive database of milk protein-derived bioactive peptides and novel visualization

“…4C), and hydrolysis of this section can result in several peptides with antimicrobial activity (Zucht, Raida, Adermann, Mägert, & Forssmann, 1995). The positively charged amino acids in this sequence (e.g., lysine 197, lysine 199, and arginine 205) are important for the antimicrobial function (Alvarez-Ordóñez, Begley, Clifford, Deasy, Considine, & Hill, 2013). …”

Virtual screening of a milk peptide database for the identification of food‐derived antimicrobial peptides

“…In a similar way, the peptide ␣ S1casein 95-104 exhibited a weak activity against E. coli NEB5␣ and B. subtilis ATCC6051, while the loss of Lys103 and Tyr104 from the C-terminus (␣ S1 -casein 95-102 ) abolished the inhibitory activity completely (Table 1). Finally, the peptides ␣ S1 -casein [16][17][18][19][20][21][22][23] and ␣ S1 -casein [17][18][19][20][21][22][23][24] , which also show sequence overlapping with isracidin, failed to exhibit antimicrobial activity.…”

“…Referring to the net charges and MICs of peptide candidates in Table 1, ␣ S2 -casein 151-181 and ␣ S2 -casein 182-207 had the highest positive charge and showed the highest inhibitory activity against the tested bacteria. Most of the neutral peptides failed to inhibit the bacterial growth, such as ␣ S1 -casein 93-100 and ␣ S1 -casein [17][18][19][20][21][22][23][24] . Four neutral peptides turned out to be relatively weak inhibitors and only one of them, ␣ S1 -casein 94-100, exerted moderate activity against the three test strains.…”

Use of Peptide Libraries for Identification and Optimization of Novel Antimicrobial Peptides

“…On the other hand, replacement of proline at positions 14 and 20 increased the potency. In addition enrichment of the peptide with Trp and Phe residues resulted in increased potency towards L. monocytogenes, however displayed no such effect against C. sakazakii [133].…”