Structurally Intact Pap and Reg Proteins Are Critical for Their Immunologic, but Not Mitogenic, Effects

Comparison of His and GST tagged versions of recombinant pancreatitis associated protein 2 in modulation of inflammatory responses

“…The methodology employed to obtain recombinant PAP2 is critical for this protein. Indeed, comparison with other commercially available forms of PAP2, where protein was subjected to ammonium chloride (NH 4 Cl) precipitation as part of the purification protocol, demonstrated that NH 4 Cl methodology completely abolished protein function in our macrophage stimulation assays [21]. It is clear that this protein family (Reg, PAP) requires particular handling and delicate purification methods to maintain optimal biological function.…”