Structural variations and roles of rhamnose-rich cell wall polysaccharides in Gram-positive bacteria

Guérin, H.; Kulakauskas, Saulius; Chapot‐Chartier, Marie‐Pierre

doi:10.1016/j.jbc.2022.102488

Cited by 22 publications

(22 citation statements)

References 114 publications

(235 reference statements)

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“…How deeply are rhamnosomes conserved and when did they evolve? Outside of plants, rhamnose is synthesized in bacteria ( 28, 29 ), fungi ( 30 ), protozoa ( 31 ), and some invertebrate animals ( 32 ). RHM1 homologs (composed of both BD1 and BD2) are present in unicellular algae, mosses, and angiosperms (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Protein sequences for RHM1-homologs from viridiplantae proteins were identified using NCBI blast. Protein sequences from bacteria ( 28, 29 ), fungi ( 30 ), protozoa ( 31 ), and invertebrate animals ( 32 ) were identified from previous literature, and their amino acid sequences were retrieved from Uniprot. Protein sequences used for alignment and phylogenetic tree construction are in Table S5.…”

Section: Yeast System Assaysmentioning

confidence: 99%

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Arabidopsis thaliana RHAMNOSE 1 condensate formation drives UDP-rhamnose synthesis

Field,

Dorone,

Dwyer

et al. 2024

Preprint

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Rhamnose is an essential component of the plant cell wall and is synthesized from uridine diphosphate (UDP)-glucose by the RHAMNOSE1 (RHM1) enzyme. RHM1 localizes to biomolecular condensates in plants, but their identity, formation, and function remain elusive. Combining live imaging, genetics, and biochemical approaches in Arabidopsis and heterologous systems, we show that RHM1 alone is sufficient to form enzymatically active condensates, which we name rhamnosomes. Rhamnosome formation is required for UDP-rhamnose synthesis and organ development. Overall, our study demonstrates a novel role for biomolecular condensation in metabolism and organismal development, and provides further support for how organisms have harnessed this biophysical process to regulate small molecule metabolism.

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Section: Discussionmentioning

confidence: 99%

Section: Yeast System Assaysmentioning

confidence: 99%

Arabidopsis thaliana RHAMNOSE 1 condensate formation drives UDP-rhamnose synthesis

Field,

Dorone,

Dwyer

et al. 2024

Preprint

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“…This complex polymer, considered to be the equivalent of wall teichoic acids (WTA), may have a more important role than that of bacterial cell wall biogenesis. Roles such as phage receptor or immune response escape are attributed to it (Guerin et al, 2022; Mistou et al, 2016). This RGP is the basis for the classification of S. mutans into 4 different serotypes: c, e, f and k. Serotyping is a way of rapidly identifying any different strains of S. mutans that an individual may have in his or her oral cavity.…”

Section: Introductionmentioning

confidence: 99%

Serotype and distribution of adhesion genes inStreptococcus mutansclinical isolates

Donnet,

Claisse,

Samot

2024

Preprint

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The delicate balance of the oral cavity is disrupted by hygiene and diet changes, leading to caries. The virulence ofStreptococcus mutans, a key caries pathogen, is partly explained by its rhamnose-glucose polysaccharide (RGP) and its sucrose-dependent and -independent adhesion mechanisms. This study investigates the diversity ofS. mutansthrough analysis of its RGP and the distribution of sucrose-independent adhesion proteins (SpaP, WapA, Cnm, Cbm) in Parkinson’s disease patients.In the PARKIDENT clinical trial, strains were isolated from saliva samples of Parkinson’s patients before and after oral hygiene procedures. Strains were preanalyzed by multiplex PCR for serotype and collagen-binding proteins, followed by high-throughput sequencing and bioinformatics analysis using RAST® and BLAST®. Phylogenetic analyses and protein modeling were also conducted.Of 40 patients in the Parkident clinical trial, only 24 hadStreptococcus mutansstrains isolable from salivary swabs. Serotyping revealed that over 80% of the 44 strains isolated were serotype c, in line with prevalence data in the literature. Furthermore, only SpaP types A and B were identified in the strains. The low variability observed for the WapA protein underlines its functional importance. Finally, thecbmgene was not found in any strain, and only 5 strains possessed thecnmgene, all of them serotype c.The study of these 44Streptococcus mutansstrains showed characteristics similar to other populations. Serotype c predominates, with minimal peptide variability in adhesion proteins (SpaP, WapA). Collagen-binding proteins Cnm and Cbm are rare or absent. Further research is essential for detailed strain distribution in Parkinson’s-affected and general French populations due toS. mutans’growing role in extra-oral pathologies.

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“…Tal qual a EF2198 da cepa V583, a EpaA também é homóloga da enzima TagO (NCBI ID: NP_391433), a iniciadora de formação do polímero de ramnose. 55,116 As sequências da EpaA e TagO apresentam 39.06% de identidade e Evalue 1.e −84 , em 99% de cobertura. A TagO adiciona GlcNAc-1-P ao transportador lipídico undecaprenil-fosfato (Und-P), a partir do UDP-GlcNAc.…”

Section: Epaaunclassified

“…117 Já que o modelo da EpaB foi sobreposto com a estrutura 5ndf (Figura 15), co-cristalizado com um dos produtos da reação, o UDP, foi possível identificar potenciais resíduos que interagem com essa molécula, são eles: ASN41 com o grupo amina do UDP, a TYR12 em empilhamento pi com Em bactérias que apresentam cadeias de ramnose ancoradas, é comum a presença de um loci cromossomal denominado rgp, próximo do loci rml. 116 Os genes rgp codificam algumas ramnosil e glicosiltransferases. O trabalho de Guerardel et al, 2020, 55 propõe que a EpaB em Enterococcus faecalis V583 adicione o primeiro resíduo de ramnose ao GlcNAc-P-P-Und, a partir da dTDP-ramnose sintetizada pelas enzimas EpaEFGH.…”

Section: Epabunclassified

>i<Enterococcus faecalis>/i<: >i<Cluster>/i< epa

Clementino

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Como ninguém chega a lugar nenhum sozinho, aqui está minha lista de agradecimento às pessoas e agências de fomento à pesquisa acadêmica, sem as quais eu definitivamente não teria escrito esta tese. Agradecer é só o que me resta.Agradeço à Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) pelo auxílio financeiro, processo nº 2017/24901-8, assim como ao CNPq e CAPES. Agradeço também aos funcionários da Universidade de São Paulo de São Carlos pela gestão e manutenção do local onde desenvolvi minha pesquisa.Agradeço ao professor Leonardo de Boni por sempre estar aberto a me ajudar na área de fotônica, além de ser uma pessoa muito agradável e simpática para um roqueiro, ao Prof. Dr. Francisco Guimarães pela ajuda com medidas confocais, à técnica Dra Andressa Patricia Alves Pinto por sua solicitude, os experimentos envolvendo o azul de molibdênio não ocorreriam sem ela me fornecer 3g do molibdato de amônio presente no Grupo de Biofísica. Agradeço ao Dr. Vinícius Ramos, por, dentre as discussões experimentais, sugerir a técnica de detecção de fosfato a partir do azul de molibdênio, à Dr. Luana Mourão, por sua disposição em ajudar todos os alunos do professor Alessandro durante a transição entre o Grupo de Biologia Molecular para o Grupo de Cristalografia. Muito obrigada.

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Structural variations and roles of rhamnose-rich cell wall polysaccharides in Gram-positive bacteria

Cited by 22 publications

References 114 publications

Arabidopsis thaliana RHAMNOSE 1 condensate formation drives UDP-rhamnose synthesis

Arabidopsis thaliana RHAMNOSE 1 condensate formation drives UDP-rhamnose synthesis

Serotype and distribution of adhesion genes inStreptococcus mutansclinical isolates

>i<Enterococcus faecalis>/i<: >i<Cluster>/i< epa

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