Structural Study on Teichoic Acids of Listeria monocytogenes Types 4a and 4d1

Fujii, H.; Kamisango, Keiichi; Nagaoka, Masato; Uchikawa, K.; Sekikawa, Isao; Yamamoto, K; Azuma, Ichiro

doi:10.1093/oxfordjournals.jbchem.a135130

Cited by 26 publications

(41 citation statements)

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“…This category contains genes that are known or putatively associated with teichoic acid biosynthesis, genes encoding wall anchored proteins, and genes associated with wall biosynthesis. Teichoic acids are a major determinant of the somatic antigens in L. monocytogenes strains (20,22,38,45,55). One of the serotype 4b-specific RDs (RD13) encodes the gtcA gene that is necessary for galactose decoration of the teichoic acid of serotype 4 strains (45).…”

Section: Resultsmentioning

confidence: 99%

Genome Diversification in Phylogenetic Lineages I and II ofListeria monocytogenes: Identification of Segments Unique to Lineage II Populations

Zhang¹,

Ju²,

Nietfeldt³

et al. 2003

J Bacteriol

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Thirteen different serotypes of Listeria monocytogenes can be distinguished on the basis of variation in somatic and flagellar antigens. Although the known virulence genes are present in all serotypes, greater than 90% of human cases of listeriosis are caused by serotypes 1/2a, 1/2b, and 4b and nearly all outbreaks of food-borne listeriosis have been caused by serotype 4b strains. Phylogenetic analysis of these three common clinical serotypes places them into two different lineages, with serotypes 1/2b and 4b belonging to lineage I and 1/2a belonging to lineage II. To begin examining evolution of the genome in these serotypes, DNA microarray analysis was used to identify lineage-specific and serotype-specific differences in genome content. A set of 44 strains representing serotypes 1/2a, 1/2b, and 4b was probed with a shotgun DNA microarray constructed from the serotype 1/2a strain 10403s. Clones spanning 47 different genes in 16 different contiguous segments relative to the lineage II 1/2a genome were found to be absent in all lineage I strains tested (serotype 4b and 1/2b) and an additional nine were altered exclusively in 4b strains. Southern hybridization confirmed that conserved alterations were, in all but two loci, due to absence of the segments from the genome. Genes within these contiguous segments comprise five functional categories, including genes involved in synthesis of cell surface molecules and regulation of virulence gene expression. Phylogenetic reconstruction and examination of compositional bias in the regions of difference are consistent with a model in which the ancestor of the two lineages had the 1/2 somatic serotype and the regions absent in the lineage I genome arose by loss of ancestral sequences.Listeria monocytogenes is a ubiquitous gram-positive bacterium that can cause life-threatening infections including meningitis, septicemia, abortion, and fetal death. Its primary route of transmission to humans is through contaminated food and despite the relatively low incidence of listeriosis in humans, outbreaks of listeriosis often have high associated morbidity, particularly among pregnant women, unborn fetuses, and immunocompromised individuals (24). These characteristics, coupled with its physiological durability and its ubiquitous distribution in nature have propelled L. monocytogenes to the forefront of food safety research and the regulatory arena.Pathogenesis of listeriosis is a consequence of the organism's ability to invade and replicate within several different cell types in mammalian tissues, including intestinal, liver, and neural tissues. During the course of food-borne infections, the bacteria penetrate the intestinal lining through cell invasion and translocation and use the lymphatic system as a conduit to reach the main target tissues within the liver and spleen (41). Prolonged replication in the liver, facilitated by depressed cell mediated immunity, is thought to be an antecedent to spread of the bacteria to brain tissue and breach of the placental barrier in pregnant wo...

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Section: Resultsmentioning

confidence: 99%

Genome Diversification in Phylogenetic Lineages I and II ofListeria monocytogenes: Identification of Segments Unique to Lineage II Populations

Zhang¹,

Ju²,

Nietfeldt³

et al. 2003

J Bacteriol

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“…Ribitol-type WTA is present in the cell walls of B. subtilis, S. aureus, and Listeria monocytogenes as well as L. plantarum, and the WTA structures of these organisms have been elucidated, 17,18) but multiple glucosylated monomers such as F1 and F2 are unknown outside L. plantarum NRIC 1068. So far, several rare WTA structures have been found in other organisms, which contain arabitol, erythritol, or mannitol in the main chain and are modified by N-acetylglucosamine or 6-O-alanylglucose.…”

Section: Discussionmentioning

confidence: 99%

Structures of Two Monomeric Units of Teichoic Acid Prepared from the Cell Wall ofLactobacillus plantarumNRIC 1068

Tomita¹,

Furihata²,

Nukada³

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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“…After their translocation across the cytoplasmic membrane through an ABC transporter (38), polymerized TA is covalently attached to the peptidoglycan via this linkage unit. The TA of L. monocytogenes is a poly(ribitol phosphate) carrying glycosidic substitutions on ribitol residues (19,24,30).…”

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Identification of an Essential Gene ofListeria monocytogenesInvolved in Teichoic Acid Biogenesis

et al. 2006

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Listeria monocytogenes is a facultative intracellular gram-positive bacterium responsible for severe opportunistic infections in humans and animals. We had previously identified a gene encoding a putative UDP-Nacetylglucosamine 2-epimerase, a precursor of the teichoic acid linkage unit, in the genome of L monocytogenes strain EGD-e. This gene, now designated lmo2537, encodes a protein that shares 62% identity with the cognate epimerase MnaA of Bacillus subtilis and 55% identity with Cap5P of Staphylococcus aureus. Here, we addressed the role of lmo2537 in L. monocytogenes pathogenesis by constructing a conditional knockout mutant. The data presented here demonstrate that lmo2537 is an essential gene of L. monocytogenes that is involved in teichoic acid biogenesis. In vivo, the conditional mutant is very rapidly eliminated from the target organs of infected mice and thus is totally avirulent.

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Structural Study on Teichoic Acids of Listeria monocytogenes Types 4a and 4d1

Cited by 26 publications

References 0 publications

Genome Diversification in Phylogenetic Lineages I and II ofListeria monocytogenes: Identification of Segments Unique to Lineage II Populations

Genome Diversification in Phylogenetic Lineages I and II ofListeria monocytogenes: Identification of Segments Unique to Lineage II Populations

Structures of Two Monomeric Units of Teichoic Acid Prepared from the Cell Wall ofLactobacillus plantarumNRIC 1068

Identification of an Essential Gene ofListeria monocytogenesInvolved in Teichoic Acid Biogenesis

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