Structural study of the sugar chains of human leukocyte common antigen CD45

Satô, Takeshi; Furukawa, Kiyoshi; Autero, Matti; Gahmberg, Carl G.; Kobata, Akira

doi:10.1021/bi00210a019

Cited by 53 publications

(33 citation statements)

References 51 publications

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“…3C). In this case, galectin-1 may bind to other CD45 glycans that bear the lactosamine sequences (13,34,52,53). However, the interaction of 35 stromal cell galectin-1 with CD45 on T cells appeared markedly reduced on BW5147 cells lacking the C2GnT (Fig.…”

Section: Discussionmentioning

confidence: 98%

CD45 Modulates Galectin-1-Induced T Cell Death: Regulation by Expression of Core 2O-Glycans

Nguyen¹,

Evans²,

Galvan³

et al. 2001

The Journal of Immunology

185

187

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Galectin-1 induces death of immature thymocytes and activated T cells. Galectin-1 binds to T cell-surface glycoproteins CD45, CD43, and CD7, although the precise roles of each receptor in cell death are unknown. We have determined that CD45 can positively and negatively regulate galectin-1-induced T cell death, depending on the glycosylation status of the cells. CD45+ BW5147 T cells lacking the core 2 β-1,6-N-acetylglucosaminyltransferase (C2GnT) were resistant to galectin-1 death. The inhibitory effect of CD45 in C2GnT− cells appeared to require the CD45 cytoplasmic domain, because Rev1.1 cells expressing only CD45 transmembrane and extracellular domains were susceptible to galectin-1 death. Moreover, treatment with the phosphotyrosine-phosphatase inhibitor potassium bisperoxo(1,10-phenanthroline)oxovanadate(V) enhanced galectin-1 susceptibility of CD45+ T cell lines, but had no effect on the death of CD45− T cells, indicating that the CD45 inhibitory effect involved the phosphatase domain. Expression of the C2GnT in CD45+ T cell lines rendered the cells susceptible to galectin-1, while expression of the C2GnT in CD45− cells had no effect on galectin-1 susceptibility. When CD45+ T cells bound to galectin-1 on murine thymic stromal cells, only C2GnT+ T cells underwent death. On C2GnT+ cells, CD45 and galectin-1 co-localized in patches on membrane blebs while no segregation of CD45 was seen on C2GnT− T cells, suggesting that oligosaccharide-mediated clustering of CD45 facilitated galectin-1-induced cell death.

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Section: Discussionmentioning

confidence: 98%

CD45 Modulates Galectin-1-Induced T Cell Death: Regulation by Expression of Core 2O-Glycans

Nguyen¹,

Evans²,

Galvan³

et al. 2001

The Journal of Immunology

185

187

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“…However, it has been reported that CD45 expressed on T and B cells is a major protein that reacts with anti-i and anti-I antibodies (28,29) and contains mainly bi-, tri-, and tetraantennary complex-type sugar chains. Tetraantennary complex-type N-glycan (30) and O-glycan (28, 31) on CD45 possess polylactosamine. We investigated the glycans on CD45 molecules by lectin microarray analysis (data not shown) and found LEL signals on anti-CD45 mAb immunoprecipitates from WT leukocytes.…”

Section: Discussionmentioning

confidence: 99%

Polylactosamine on glycoproteins influences basal levels of lymphocyte and macrophage activation

Togayachi

Kozono

Ishida

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

104

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␤1,3-N-acetylglucosaminyltransferase 2 (␤3GnT2) is a polylactosamine synthase that synthesizes a backbone structure of carbohydrate structures onto glycoproteins. Here we generated ␤3GnT2-deficient (␤3GnT2 ؊/؊ ) mice and showed that polylactosamine on N-glycans was markedly reduced in their immunological tissues. In WT mice, polylactosamine was present on CD28 and CD19, both known immune costimulatory molecules. However, polylactosamine levels on these molecules were reduced in ␤3GnT2 ؊/؊ mice. ␤3GnT2 ؊/؊ T cells lacking polylactosamine were more sensitive to the induction of intracellular calcium flux on stimulation with anti-CD3 /CD28 and proliferated more strongly than T cells from WT mice. ␤3GnT2 ؊/؊ B cells also showed hyperproliferation on BCR stimulation. Macrophages from ␤3GnT2 ؊/؊ mice had higher cell surface CD14 levels and enhanced responses to endotoxin. These results indicate that polylactosamine on Nglycans is a putative immune regulatory factor presumably suppressing excessive responses during immune reactions.␤-1,3-N-acetylglucosaminyltransferase ͉ glycosyltransferase ͉ hyperactivation ͉ immune response

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“…Kishihara, K., Penninger, J., Wallace, V. A., Kundig, T. M., Kawai, to the multiply sialylated complex-type than the mono-sialylated K., Wakeham, A., Timms, E., Pfeffer, K., Ohashi, P. S., Thomas, oligosaccharides [31]. complex-type with and without poly(N-acetyllactosamine) [28]. by the electrophoretic mobility of them, and may not interact …”

Section: Discussionmentioning

confidence: 99%

“…Structural studies of the N-linked sugar chains of CD45 from human peripheral blood leukocytes revealed that they are membrane and cytoplasmic domains [17Ϫ19]. The individual isoforms showed differences in the interaction with CD4-T-cell mostly of complex-type with highly branched tri-and tetra-antennary structures and poly(N-acetyllactosamine) units [28]. antigen receptor-complex [20] and in the activation of intracellular signals [21Ϫ24].…”

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confidence: 99%

Structural study of the O‐linked sugar chains of human leukocyte tyrosine phosphatase CD45

Furukawa

Funakoshi

Autero

et al. 1998

European Journal of Biochemistry

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The O-linked sugar chains of the human leukocyte cell surface glycoprotein CD45 were released as tritium-labeled oligosaccharides by β-elimination in the presence of NaB 3 H4. Mono Q column chromatography revealed that they comprise neutral (64%) and acidic (36%) oligosaccharides, the latter of which were converted to neutral ones by Arthrobacter ureafaciens sialidase treatment. Structural studies of each oligosaccharide fractionated on a Bio-Gel P-4 column by sequential exoglycosidase digestion and by methylation analysis revealed that human leukocyte CD45 contains mainly core 1 and core 2 oligosaccharides, 15% of which are modified with poly (N-acetyllactosamine) chains in different extensions. CD45 consists of several isoforms which were isolated after cell surface sialic acid residues were labeled by periodate/NaB 3 H 4 treatment. Bio-Gel P-6 column chromatography of a mixture of the tritium-labeled glycopeptide/oligosaccharides obtained by pronase-digestion followed by mild alkaline borohydride treatment showed that distribution of the sialylated core 2 oligosaccharides is different among CD45 isoforms.Keywords : human leukocyte; CD45 ; O-linked sugar chain.CD45 is a receptor-like leukocyte cell surface glycoprotein and may be involved in apoptosis of T-lineage cells [25]. Similarly, mannose residues of CD45 expressed on mouse immature with protein tyrosine phosphatase activity in its cytoplasmic domain [1Ϫ6]. Analysis of mature T-cell and B-cell lines deficient thymocytes are recognized by the serum mannan-binding protein, and may be important for the development and maturation in the expression of CD45 revealed that the activation signals mediated by T-cell antigen receptor and B-cell antigen receptor of thymocytes [26], presumably by interaction with DEC-205, a membrane-bound protein which is homologous to the macroare impaired [7Ϫ11]. Similarly, in CD45-gene disrupted mutant mice, the maturation of T cells was severely disturbed and the phage mannose receptor, expressed on thymic dendritic cells [27]. The sialylated N-linked sugar chains of CD45 contain only peripheral T cells failed to respond to T-cell-antigen-receptorϪ mediated signaling [12]. These results indicate that CD45 is in-A-2,6-linked sialic acid residues [28] and may be involved in cell adhesion mediated by the Ig superfamily member CD22 [29], a dispensable for exerting antigen-specific activation of T and B cells. CD45 exists as multiple isoforms generated from the sialic-acid-binding lectin [30]. Binding studies of oligosaccharides with different sialylation levels to a column immobilized single gene by alternate splicing of exons which encode a part of the extracellular domain [13Ϫ16]. The expression of distinct with CD22-IgG chimeric protein revealed that CD22 has higher affinity toward highly sialylated complex-type oligosaccharides CD45 isoforms varies among T-cell subgroups and through Tcell ontogeny, but all the isoforms contain the identical trans- [31]. Structural studies of the N-linked sugar chains of CD45 from human peripheral...

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Structural study of the sugar chains of human leukocyte common antigen CD45

Cited by 53 publications

References 51 publications

CD45 Modulates Galectin-1-Induced T Cell Death: Regulation by Expression of Core 2O-Glycans

CD45 Modulates Galectin-1-Induced T Cell Death: Regulation by Expression of Core 2O-Glycans

Polylactosamine on glycoproteins influences basal levels of lymphocyte and macrophage activation

Structural study of the O‐linked sugar chains of human leukocyte tyrosine phosphatase CD45

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