Structural study in AOT reverse micelle containing native and modified α-chymotrypsin

Pitre, Franck; Pileni, Marie‐Paule

doi:10.1007/bfb0118595

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…Another surfactant scrutinized to study its self-assembly in the gas phase is sodium bis (2-ethylhexyl) sulfosuccinate (AOTNa); this surfactant is characterized by the presence of two hydrophobic chains that, in apolar solution, guarantee the reverse micelle formation (Figure 2). [25][26][27][28][29] AOTNa was first investigated using FAB-MS by Lyon et al, 17 both in positive and in negative ion mode. The observed spectra were mainly characterized by prominent peaks due to monomeric species; [AOTNa + Na] + at m/z 467 and [AOT]at m/z 421, respectively.…”

Section: The Mirror Effectmentioning

confidence: 99%

Mass Spectrometry of Surfactant Aggregates

Ceraulo̊

Giorgi

Liveri

et al. 2011

Eur J Mass Spectrom (Chichester)

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In contrast with the enormous amount of literature produced during many decades in the field of surfactant aggregation in liquid, liquid crystalline and solid phases, only a few investigations concerning surfactant self- assembling in the gas phase as charged aggregates have been carried out until now. This lack of interest is disappointing in view of the remarkable theoretical and practical importance of the inherent knowledge. The absence of surfactant-solvent interactions makes it easier to study the role of surfactant-surfactant forces in determining their peculiar self-assembling features as well as the ability of these assemblies to incorporate selected solubilizate molecules. Thus, the study of gas-phase surfactant and surfactant-solubilizate aggregates is a research subject which has exciting potential, including mass and energy transport in the atmosphere, origin of life and simulation of supramolecular aggregation in interstellar space. On the other hand, the structural and dynamic properties of surfactant aggregates in the gas phase could be exploited in a number of interesting applications such as atmospheric cleaning agents, transport and protection of pulmonary drugs or biomolecules and as nanoreactors for specialized chemical reactions in confined space. Spectrometric techniques, together with molecular dynamics simulations, have been the principal investigative tools in this field and appearto be particularly suited to gaining fundamental information on the structure and stability of surfactant-based supramolecular aggregates, charge state effects, entrapment of solubilizate molecules, preferential solubilization sites and chemical reactions localized in a single organized aggregate. The main aim of this review is to present the actual state of the art in this novel and exciting research field underlining the knowledge acquired up to now as well as the aspects needing a more deep understanding. Moreover, intriguing departures of the behavior of surfactant solutions under electrospray ionization conditions from that of ionic, polar and apolar analytes will be discussed.

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Section: The Mirror Effectmentioning

confidence: 99%

Mass Spectrometry of Surfactant Aggregates

Ceraulo̊

Giorgi

Liveri

et al. 2011

Eur J Mass Spectrom (Chichester)

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“…Recently, protein-RM systems have been proposed to study the structure and dynamics of large biomolecular systems, taking advantage of the fast tumbling time of protein-containing RM dissolved in low viscosity nonpolar solvents like CO 2 or isooctane (3)(4)(5). Proof of principle studies on ubiquitin, a-chymotrypsin, and flavodoxin have shown that the protein structure does not change significantly, although there are some changes in the dynamics (3)(4)(5). Early studies of proteins in RM have shown that the proteins are active and that the enzymatic activity is largely maintained (6).…”

mentioning

confidence: 99%

An Alpha-Helical Peptide in AOT Micelles Prefers to be Localized at the Water/Headgroup Interface

Tian

Garcı́a

2009

Biophysical Journal

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A model alpha-helical peptide encapsulated in a reverse micelle is used to study the structure and dynamics of proteins under constrained environments that mimic the membrane-water environment in cells. Molecular dynamics simulations of the self assembly of systems composed of a peptide, sodium bis(2-ethylhexyl) sulfosuccinate (AOT), water, and isooctane show that the peptide prefers to be located at the water/AOT headgroups interface. We explore the effect of the AOT headgroup charge and the peptide charge and find that the peptides migrate to the interface in all cases. These results show that the peptides prefer the constrained hydration environment of the AOT headgroups. The driving force for this configuration is the gain in entropy by released water molecules that otherwise would solvate the protein and surfactant headgroups.

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“…Several papers deal with the self-assembling in gas phase of sodium bis (2-ethylhexyl) sulfosuccinate (AOTNa), a surfactant characterized by the presence of two hydrophobic chains. In apolar solvent, this surfactant is able to form reverse micelles while in water, direct ones (Maitra & Eicke, 1981;Jain, Varshney, & Maitra, 1989;Pitr e & Pileni, 1993;Moran et al, 1995;Abuin et al, 2002).…”

Section: B Experimental Evidences Of Surfactant Aggregation In Gas Pmentioning

confidence: 99%

Charged supramolecular assemblies of surfactant molecules in gas phase

Bongiorno

Ceraulo̊

Indelicato

et al. 2015

Mass Spectrometry Reviews

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The aim of this review is to critically analyze recent literature on charged supramolecular assemblies formed by surfactant molecules in gas phase. Apart our specific interest on this research area, the stimuli to undertake the task arise from the widespread theoretical and applicative benefits emerging from a comprehensive view of this topic. In fact, the study of the formation, stability, and physicochemical peculiarities of non-covalent assemblies of surfactant molecules in gas phase allows to unveil interesting aspects such as the role of attractive, repulsive, and steric intermolecular interactions as driving force of supramolecular organization in absence of interactions with surrounding medium and the size and charge state dependence of aggregate structural and dynamical properties. Other interesting aspects worth to be investigated are joined to the ability of these assemblies to incorporate selected solubilizates molecules as well as to give rise to chemical reactions within a single organized structure. In particular, the incorporation of large molecules such as proteins has been of recent interest with the objective to protect their structure and functionality during the transition from solution to gas phase. Exciting fall-out of the study of gas phase surfactant aggregates includes mass and energy transport in the atmosphere, origin of life and simulation of supramolecular aggregation in the interstellar space. Moreover, supramolecular assemblies of amphiphilic molecules in gas phase could find remarkable applications as atmospheric cleaning agents, nanosolvents and nanoreactors for specialized chemical processes in confined space. Mass spectrometry techniques have proven to be particularly suitable to generate these assemblies and to furnish useful information on their size, size polydispersity, stability, and structural organization. On the other hand molecular dynamics simulations have been very useful to rationalize many experimental findings and to furnish a vivid picture of the structural and dynamic features of these aggregates. Thus, in this review, we will focus on the most important achievements gained in recent years by both these investigative tools.

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Structural study in AOT reverse micelle containing native and modified α-chymotrypsin

Cited by 4 publications

References 5 publications

Mass Spectrometry of Surfactant Aggregates

Mass Spectrometry of Surfactant Aggregates

An Alpha-Helical Peptide in AOT Micelles Prefers to be Localized at the Water/Headgroup Interface

Charged supramolecular assemblies of surfactant molecules in gas phase

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