Structural Studies on the Second Mycobacterium smegmatis Dps: Invariant and Variable Features of Structure, Assembly and Function

M. tuberculosis Pantothenate Kinase: Dual Substrate Specificity and Unusual Changes in Ligand Locations

“…4,5 The structures of the enzyme in the CoA complex and in the complexes involving AMPPNP and ADP also show significant differences. 4,5 We have been exploring the structures and interactions of PanK from Mycobacterium tuberculosis (MtPanK) as part of a program in this laboratory [6][7][8][9][10][11][12] and an international effort involving structural studies on mycobacterial proteins. [13][14][15][16][17] The CoA complex of MtPanK has a structure very similar to that of the corresponding EcPanK complex.…”

Campylobacter jejuni Dps Protein Binds DNA in the Presence of Iron or Hydrogen Peroxide

“…Since this pioneer study, many different Dps proteins have been characterized. These proteins are structurally conserved and widely distributed in prokaryotes (8,9). Unlike typical ferritins that have 24 subunits and 432 symmetry, Dps proteins assemble in a quasispherical dodecamer with 23 symmetry and can store ϳ500 iron atoms (4,10).…”

A Histidine Aspartate Ionic Lock Gates the Iron Passage in Miniferritins from Mycobacterium smegmatis

“…MsDps1 is the only Dps that has been shown to exist as a trimer and a dodecamer in solution (55). MsDps2 on the other hand exhibits a stable dodecameric form in vitro (23). In this study, we have used as our model MsDps2 to study the mechanism of iron uptake and release in mycobacterial Dps molecules.…”

“…A search of TIGR database with the MsDps1 sequence led to the identification of the second Dps namely MsDps2 (23). MsDps1 has a long C-terminal tail rich in positively charged residues for DNA binding unlike MsDps2, which lacks any N-or C-terminal extensions, despite which it can still bind to DNA.…”

“…The protein is roughly spherical with a hollow core and pores at the 3-fold axes. The dodecamer exhibits two types of 3-fold interfaces namely, the ferritin-like interface and the dps-like interface (23). The ferritin-like symmetrical interface is thought to channel iron into the protein cavity.…”