Structural Studies on Teichoic Acids in Cell Walls of Several Serotypes of Listeria monocytogenes1

Uchikawa, K.; Sekikawa, Isao; Azuma, Ichiro

doi:10.1093/oxfordjournals.jbchem.a135486

Cited by 74 publications

(87 citation statements)

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“…A total of 18 of 29 Listeria strains tested were lysed by P70, including food isolates, reference strains, and the novel species L. marthii (9) but not L. rocourtiae (17). Consistent with other Listeria phages isolated so far, P70 does not bind to or lyse serovar 3 strains that lack the rhamnose residue in the polyribitol phosphate backbone of wall teichoic acids (7,26).…”

mentioning

confidence: 80%

Bacteriophage P70: Unique Morphology and Unrelatedness to Other Listeria Bacteriophages

Schmuki

Erne

Loessner

et al. 2012

J Virol

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Listeria monocytogenes is an important food-borne pathogen, and its bacteriophages find many uses in detection and biocontrol of its host. The novel broad-host-range virulent phage P70 has a unique morphology with an elongated capsid. Its genome sequence was determined by a hybrid sequencing strategy employing Sanger and PacBio techniques. The P70 genome contains 67,170 bp and 119 open reading frames (ORFs). Our analyses suggest that P70 represents an archetype of virus unrelated to other known Listeria bacteriophages. Listeria monocytogenes is a Gram-positive food-borne pathogen that causes the rare but severe disease listeriosis, which is characterized by a high mortality rate (6, 28). Although many Listeria-specific bacteriophages have been described, only a limited number have been fully sequenced (2,5,15,21,29). Most Listeria phages are temperate siphoviruses, with B054, A511, and P100 being the only described myoviruses (2,5,15). Listeria can be efficiently detected and controlled by the use of highly virulent and specific bacteriophages (2, 22, 23). Bacteriophage-based biocontrol of food-borne pathogens has received increasing interest in recent years, due to its practicability, efficacy, safety, and low cost (8, 10-12). In our search for additional virulent phages useful for biocontrol, we discovered phage P70, which turned out to be a novel type of Listeriaspecific bacterial virus.Phage P70 was isolated from a grass silage sample from a dairy farm in Homburg, Switzerland. Samples taken were amplified using L. monocytogenes WSLC1001 and WSLC1042 as phage hosts, and potential phage in the resulting lysates was assessed by the soft agar overlay method (16,27). Phage P70 was propagated on semiconfluent soft agar plates of Listeria ivanovii WSLC3009 (16). Purified phage stocks were obtained by polyethylene glycol (PEG) precipitation and density gradient centrifugation (15,24). Hostrange analysis was performed as described elsewhere (18,19), and P70 was found to feature an unusually broad host range within the genus Listeria, infecting serovars 1/2a, 1/2b, 1/2c, 4a, 4c, 4d, 4e, 5, 6a, and 6b with similar efficiencies. A total of 18 of 29 Listeria strains tested were lysed by P70, including food isolates, reference strains, and the novel species L. marthii (9) but not L. rocourtiae

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mentioning

confidence: 80%

Bacteriophage P70: Unique Morphology and Unrelatedness to Other Listeria Bacteriophages

Schmuki

Erne

Loessner

et al. 2012

J Virol

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“…The main components of the listerial cell wall are PG, LPS, and TAs (253). The WTAs of serovars 1/2, 3, and 7 are decorated with GlcNAc or Rha residues, while GlcNAc is incorporated into the backbone of the WTAs of serovars 4, 5, and 6, and the GlcNAc is again decorated with Glc and Gal residues (254).…”

Section: Fig 6 O-antigen Lps Biosynthesis and Lps Assembly And Transpmentioning

confidence: 99%

The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates

Tytgat

Lebeer

2014

Microbiol Mol Biol Rev

128

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SUMMARY Humans have been increasingly recognized as being superorganisms, living in close contact with a microbiota on all their mucosal surfaces. However, most studies on the human microbiota have focused on gaining comprehensive insights into the composition of the microbiota under different health conditions (e.g., enterotypes), while there is also a need for detailed knowledge of the different molecules that mediate interactions with the host. Glycoconjugates are an interesting class of molecules for detailed studies, as they form a strain-specific barcode on the surface of bacteria, mediating specific interactions with the host. Strikingly, most glycoconjugates are synthesized by similar biosynthesis mechanisms. Bacteria can produce their major glycoconjugates by using a sequential or an en bloc mechanism, with both mechanistic options coexisting in many species for different macromolecules. In this review, these common themes are conceptualized and illustrated for all major classes of known bacterial glycoconjugates, with a special focus on the rather recently emergent field of glycosylated proteins. We describe the biosynthesis and importance of glycoconjugates in both pathogenic and beneficial bacteria and in both Gram-positive and -negative organisms. The focus lies on microorganisms important for human physiology. In addition, the potential for a better knowledge of bacterial glycoconjugates in the emerging field of glycoengineering and other perspectives is discussed.

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“…Most of the descriptions of the composition and suspected structure of the L. monocytogenes membrane, peptidoglycan, and associated polymers were made about 20 to 40 years ago (57,65,171,180,181). However, this topic now is receiving further attention, which should help to refine the overall structure (for a review, see reference 145).…”

Section: The L Monocytogenes Cellular Envelopementioning

confidence: 99%

Listeria monocytogenesSurface Proteins: from Genome Predictions to Function

Bierne

Cossart

2007

Microbiol Mol Biol Rev

210

257

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SUMMARY The genome of the human food-borne pathogen Listeria monocytogenes is predicted to encode a high number of surface proteins. This abundance likely reflects the ability of this bacterium to survive in diverse environments, including soil, food, and the human host. This review focuses on the various mechanisms by which listerial proteins are attached at the bacterial surface and their many functions, including peptidoglycan metabolism, protein processing, adhesion to host cells, and invasion of host tissues. Extensive in silico analysis of the domains or motifs present in these mosaic proteins reveals that diverse structural features allow the surface proteome to interact with diverse bacterial or host components. This diversity offers new clues about the molecular bases of Listeria pathogenesis.

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Structural Studies on Teichoic Acids in Cell Walls of Several Serotypes of Listeria monocytogenes1

Cited by 74 publications

References 0 publications

Bacteriophage P70: Unique Morphology and Unrelatedness to Other Listeria Bacteriophages

Bacteriophage P70: Unique Morphology and Unrelatedness to Other Listeria Bacteriophages

The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates

Listeria monocytogenesSurface Proteins: from Genome Predictions to Function

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