Structural studies of the O-polysaccharide from the lipopolysaccharide of Moraxella (Branhamella) catarrhalis serotype A (strain ATCC 25238)

Edebrink, Per; Jansson, Per‐Erik; Rahman, Motiur; Widmalm, Göran; Holme, Tord; Weintraub, Andrej

doi:10.1016/0008-6215(94)80040-5

Cited by 71 publications

(58 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The outer membrane of B. catarrhalis contains LOS, which lacks the long O-polysaccharide side chains observed in enteric gram-negative bacteria (51,61). In this regard, the LOS of B. catarrhalis resembles other nonenteric gram-negative bacteria.…”

Section: Lipooligosaccharidementioning

confidence: 99%

Lower respiratory tract infection

Sharland¹

2016

OSH Manual of Childhood Infections

View full text Add to dashboard Cite

Section: Lipooligosaccharidementioning

confidence: 99%

Lower respiratory tract infection

Sharland¹

2016

OSH Manual of Childhood Infections

View full text Add to dashboard Cite

“…Differences in terminal sugars appear to constitute the basis for the antigenic specificities of the three serotypes. A terminal a -~-GlcNAc-( 1 -2)-p-D-Glc was found to be specific for serotype A LPS [2,3], a terminal /?-D-Gal-( 1-4)-p-DGlc for serotype B (unpublished observation) and a terminal P-D-Gal-( 1 -4)-a-~-GlcNAc for serotype C [13]. The common inner core of the three serotypes contains a terminal a-D-Gal-( 1 -4)-/?-~-Gal-( 1 -4)-a-Glc epitope [2].…”

Section: Introductionmentioning

confidence: 95%

“…Each LPS preparation was run in SDS-PAGE as described previously [2] and the gel was equilibrated in cold transfer buffer (25 m~ Tris-HC1 containing 192 m~ glycine, pH 8.3) for 30 min. Electrophoretic transfer of the sample to a nitrocellulose membrane was done at lOOV, 250mA for 1 h at 4°C.…”

Section: Sds-page and Immunoblottingmentioning

confidence: 99%

“…Three major antigenic types can be distinguished, accounting for 95% of all strains. Structural studies have shown that the carbohydrate parts of the LPS of the different Corresponding author: Dr M. Rahman serotypes consist of branched polysaccharides with a common inner core [2,131. Differences in terminal sugars appear to constitute the basis for the antigenic specificities of the three serotypes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antibody response in rabbits to serotype-specific determinants in lipopolysaccharides from Moraxella catarrhalis

Rahman

Holme²

1996

Journal of Medical Microbiology

View full text Add to dashboard Cite

Antibodies against the serotype determinant epitopes of Moraxella catarrhalis lipopolysaccharides (LPS) were demonstrated in sera from rabbits immunised with whole bacterial cells. Purified LPS preparations from eight strains of M. catarrhalis were used as antigens in enzyme-linked immunosorbent assays (ELISA) and immunoblotting. The serotype specificity of the antibodies was shown by neutralisation with LPS and with purified polysaccharide obtained from LPS prepared from strains belonging to different serotypes. In immunoblots, antisera against the A and B LPS serotypes reacted only with LPS of its own type, confirming the presence of type-specific antibodies. A weak band was observed with type A LPS and antibody to type C, indicating cross-reactivity between the A and C serotypes. This cross-reaction can be explained on the basis of the known chemical structure of the LPS of the different serotypes. After heterologous absorption of sera, bands were obtained with homologous LPS antigen. These results suggest that the predominant antibody response in rabbits to LPS from M. catarrhalis is serotype-specific, unlike that previously observed in infected human patients.

show abstract

“…The primary sugar sequence of the OS from each of the major serotypes has been determined [11][12][13] and reveals that all contain a common branched pentasaccharide core ( Figure 1 sugars A, B, C, E, and F) linked through two Kdo residues to lipid A. An unusual feature of this pentasaccharide inner core OS of M. catarrhalis is that it does not contain heptose residues and the inner core instead is comprised of glucose residues, one of which is directly bonded to Kdo.…”

Section: Introductionmentioning

confidence: 99%

An Unusual Carbohydrate Conformation is Evident in Moraxella catarrhalis Oligosaccharides

et al. 2015

View full text Add to dashboard Cite

Oligosaccharide structures derived from the lipooligosaccharide of M. catarrhalis show that the highly branched glucose-rich inner core of the oligosaccharide has an altered conformation compared to the most truncated tetra-glucose-Kdo lgt1/4Δ oligosaccharide structure. Addition of one residue each to the (1-4) and (1-6) chains to give the lgt2Δ oligosaccharide is the minimum requirement for this conformational change to occur. Extensive molecular modeling and NMR investigations have shown that the (1-3), (1-4), and (1-6) glycosidic linkages from the central α-D-Glcp have significantly altered conformational preferences between the two structures. For the lgt1/4Δ oligosaccharide the (1-3) and (1-4) linkage populates predominantly the syn minimum on the conformational free energy map and for the (1-6) linkage conformational flexibility is observed, which is supported by 1 H-NMR T1 measurements. For the lgt2Δ oligosaccharide the unusual "(1-4)anti-ψ(1-6)gg" conformation, which could be confirmed by long-range NOE signals, is a dominant conformation in which the oligosaccharide is very compact with the terminal α-D-GlcNAc residue folding back towards the center of the molecule leading to an extensive intra-molecular hydrophobic interaction between the terminal residues. Comparing effective H-H distances, OPEN ACCESSMolecules 2015, 20 14235 which were calculated for conformational sub-ensembles, with the NOE distances revealed that typically multiple conformations could be present without significantly violating the measured NOE restraints. For lgt2Δ the presence of more than one conformation is supported by the NOE data.

show abstract

Structural studies of the O-polysaccharide from the lipopolysaccharide of Moraxella (Branhamella) catarrhalis serotype A (strain ATCC 25238)

Cited by 71 publications

References 26 publications

Lower respiratory tract infection

Lower respiratory tract infection

Antibody response in rabbits to serotype-specific determinants in lipopolysaccharides from Moraxella catarrhalis

An Unusual Carbohydrate Conformation is Evident in Moraxella catarrhalis Oligosaccharides

Contact Info

Product

Resources

About