Structural studies of the O-antigen polysaccharide of Salmonella thompson, serogroup C1 (6,7)

Lindberg, Bengt; Leontein, Karin; Lindquist, Ulf; Svenson, Stefan B.; Wrangsell, Göran; Dell, Anne; Rogers, Mark E.

doi:10.1016/0008-6215(88)85100-0

Cited by 49 publications

(26 citation statements)

References 7 publications

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“…1) was the likely structural background for the 0 : 6, determinant. More recently Lindberg et al (1988) reported that the 0 : 6,,6,,7 PS of S. thompson strain IS40 comprises two populations of chains which differ only because one population lacks the a-D-glUCOpyranOSyl group 3-linked to the a-D-mannopyranosyl 111 residue of each repeat unit. As the non-glucosylated, phage-14-endomannosidasesusceptible population has been shown to display only the 0 : 7 specificity (Ekwall et al, 1982), it follows that chains substituted with D-glucose at the a-Dmannopyranosyl I11 residue have both the 0 : 6 , and 0 : 6, determinants.…”

Section: Discussionmentioning

confidence: 99%

O-antigenic determinants in Salmonella species of serogroup C1 are expressed in distinct immunochemical populations of chains

Nnalue

Lindberg

1997

Microbiology

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2 Karol i nska I nst it Ute,The 0-antigenic specificities found among salmonellae of serogroup C, are 0:6,,7,0:6,,7,0:6,,6,, 7 and 0:6,7,14, as defined by classical serology. Factor 0:7 is the group-wide determinant while factors O:6,, 0:6, and 0:14 are found in some strains but not others. Strains of the 0:6,,7 specificity are subject to lysogenic conversion by phages 61 and 14 to the 0:6,,7 and 0:6,7,14 specificities, respectively. To further delineate antigenic complexity and serological relationships among strains of this serogroup monoclonal antibodies (mAbs) were generated against the 0: 6,,6,,7 polysaccharide of Salmonella thompson. Five mAbs of either the O:6, or the O:6, specificities did not bind 0:6,7,14 strains or LPS, showing that the 0:6 determinant in these strains is neither O:6, nor O:6,. Thus antigenic conversion of 0:6,,7 strains by phage 14 is accompanied by addition of 0:14 as well as loss of O:6,. Three mAbs which demonstrated group-wide reactivity, and were thus specific for 0: 7, recognized clearly separable epitopes hereby defined as sub-specificities, 0: 7,, 0: 7, and 0:7,. lmmunoblotting of mAbs against electrophoretically resolved LPS showed that factors O:6, and 0:6, are expressed only in LPS molecules of high molecular mass whereas 0:7, and 0:7, are expressed only in relatively low-molecular-mass chains. These results are consistent with the expression of different antigenic determinants in structurally distinct subpopulations of 0 chains. The implication of the existence of distinct subpopulation of chains is that the published structure of the 0:6,7 repeat unit is not fully representative of the 0-antigenic structure of this group.

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Section: Discussionmentioning

confidence: 99%

O-antigenic determinants in Salmonella species of serogroup C1 are expressed in distinct immunochemical populations of chains

Nnalue

Lindberg

1997

Microbiology

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“…We also reported in 'ecent papers that the serotype A-specific fl-1,2-1inked mannose mit in the mannans of C. albicans disappeared by cultivation )fthe cells under acidic pH [10] or high temperature [11] condiions. Although a single fl-l,2-1inked mannose unit is present n the cell wall polysaccharides of several bacteria, e.g., Serratia narcescens [12] and Salmonella thompson [13], consecutive fl-L,2-1inked mannose units are contained only in the genus Can-"Corresponding author. Fax: (81) (22) dida and several other yeasts.…”

Section: ~ Introductionmentioning

confidence: 99%

Detection of β‐1,2‐mannosyltransferase in Candida albicans cells

et al. 1995

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A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% ~1 riton X-100. Incubation of this fraction with a mannopentaose, Man~l-->2Manotl-->(2Manal-Qz2Man (al~Mans), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by ~H NMR indicates that a~Man5 was changed to Man~l--~ 2Manl~ll--~2Manal-~(2Manal--~)z 2Man (a~lMan6). This gl-l,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn 2÷ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albieans NIH B-792 (serotype B) strain calls, the mannan of which does not possess both the otl3Mans and airMan6 side chains, also exhibited the same/3-1,2-ManTase II activity. /(ey words: Candida albicans; fl-1,2-Linked mannose; Mannosyltransferase ~. IntroductionCandidiasis is an infectious disease frequently seen in early hildhood and in adults with predisposing conditions such as ,tiabetes, cancer, AIDS, and immuno-suppressive therapy [1,2]. Candida albicans strains were divided into two serotypes, A and ~, by Hasenclever and Mitchell [3] from the antigenicity of cell vall mannans. A structural study of the mannans of the 2 albicans serotype A and B strains has been achieved during he last several years [4,5], and we have demonstrated the pres-'nce of phosphodiesterified fl-l,2-1inked oligomannosyl moieies as a group of common epitopes throughout the two seroype strains [6,7]. The major difference between the structural !eatures of mannans from C. albicans serotype A and B strain .',ells is the presence of a fl-1,2-1inked mannose unit attached to m ~-l,2-1inked one in the side chains of the former mannan ,train [8,9], but lacking in the latter ones. We also reported in 'ecent papers that the serotype A-specific fl-1,2-1inked mannose mit in the mannans of C. albicans disappeared by cultivation )fthe cells under acidic pH [10] dida and several other yeasts. Thus, the detection of the circulating mannan antigen containing fl-l,2-1inkages in patients' sera by immunological procedures is important for the diagnosis of invasive candidiasis. Furthermore, several workers reported that the fl-1,2-linkage-containing side chains participate in adherence of C albicans cells to mammalian cells in the initial step of Candida infection [14,15]. Therefore, we tried to detect and characterize a fl-l,2-mannosyltransferase (ManTase) which is responsible for the biosynthesis of the serotype A specific epitope of C. albicans mannan. The biosynthetic pathway of the serotype A-specific side chain can be depicted as shown in Fig. 1 based on the structural analysis findings of C. albicans mannans [4]. Namely, the first transfer of the fl-l,2-linked mannose unit from GDP-mannose to the non-reducing terminal site of the ~-l,2-1inked mannotetraosyl side chain takes place by participation of the fl-l,2-ManTase I. However,...

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“…P-l72-Mannosidic linkages have been described to date only in the bacterial species Salmonella thompson (Lindberg et al, 1988) and Serratia marcescens (Oxley & Wilkinson, 1991) and in yeast species (Kobayashi etal., 199213, 1994a;Shibata etal., 1985Shibata etal., , 1993. In C. albicans, structural analysis has shown their presence either in homopolymers of the acid-labile part of mannan in both serotypes A and B (Shibata e t al., 1989;Kobayashi e t al., 1990) and in short sequences at the reducing end of a-172-linked oligomannosides side-chains in the acid-stable part of serotype A mannan (Kobayashi etal., 1992a).…”

Section: Discussionmentioning

confidence: 99%

Evidence for different mannosylation processes involved in the association of -1,2-linked oligomannosidic epitopes in Candida albicans mannan and phospholipomannan

et al. 1996

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A monoclonal antibody specific for /?-1,2-linked oligomannosides was used to study the association of these residues with Candida albicans mannan and phospholipomannan (PLM) in relation to growth conditions and in mannan mutant strains. Double immunof luorescence assays performed on cells grown under standard conditions indicated a highly heterogeneous cell surface expression of these epitopes in comparison with the homogeneous expression of a-linked oligomannosidic epitopes. Growth in the presence of tunicamycin, which inhibits mannan N-glycosylation, resulted in an absence of / l -1, 2-oligomannosidic epitopes on the cell surface, although PLM synthesis still occurred as shown by autoradiography. Similarly, growth in acidic conditions, which inhibits the incorporation of /l-l,2-oligomannosides in mannan, resulted in an absence of ~-1,2-oligomannosidic epitopes a t the cell surface, although they still associated with PLM as shown by Western blotting. Western blots of C. albicans mutant strains with reduced amounts or an absence of phosphorus and acid-labile B-1,2-oligomannosides in their mannan confirmed that the association of /l-1,2-linked oligomannosides with mannan and with PLM involves different mannosylation processes.Keywords : p-1,2-oligomannosidic epitopes, mannan, phospholipomannan, pmannosylation processes INTRODUCTIONHomopolymers of p-1,2-linked oligomannosides were initially described in Candida albicans cell wall phosphopeptidomannan (PPM), also called mannan, where they correspond to the antigenic factor 5 (Shibata e t al., 1992).Such P-1,2-linked oligomannosidic epitopes were further observed on a C. albicans 14-18 kDa phospholipomannan (PLM) (Trinel e t al., 1992), a glycolipid that induced TNFa production by macrophages (jouault e t al., 1994). Mapping of these p-l,2-oligomannosidic epitopes among glycoconjugates of Candida species revealed that the presence of PLM was restricted to the most pathogenic species of the genus Candida, C. albicans and C. tropicalis (Cantelli e t al., 1995) and also demonstrated that in other t Present address: Facolta di Medecina e Chirurgia, lstituto di Microbiologia, Via Gramsci 14, 43100 Parma, Italy.Abbreviations: AERC, alkaline extraction in reducing conditions; ConA, concanavalin A; IFA, immunofluorescence assay; PLM, phospholipomannan; PPM, phosphopeptidomannan; TNF, tumour necrosis factor.Candida species, such as C. parapsilosis, C. krusei and C. glabrata, p-1,2-oligomannosidic epitopes were shared by numerous mannoproteins according to species-specific profiles.From a physiopathological point of view, recent observations increasingly suggest that P-1,2-oligomannosides are important molecules involved in the host-Candida interplay during pathogenic processes. P-1,2-0ligo-mannosides have been shown to bind to host cell membranes (Li & Cutler, 1993), to trigger the cytokine network (Jouault e t al., 1995) and elicit an antibody response different from those supported by a-Man residues (Poulain e t al., 1993) during candidosis. In contrast to these studies,...

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Structural studies of the O-antigen polysaccharide of Salmonella thompson, serogroup C1 (6,7)

Cited by 49 publications

References 7 publications

O-antigenic determinants in Salmonella species of serogroup C1 are expressed in distinct immunochemical populations of chains

O-antigenic determinants in Salmonella species of serogroup C1 are expressed in distinct immunochemical populations of chains

Detection of β‐1,2‐mannosyltransferase in Candida albicans cells

Evidence for different mannosylation processes involved in the association of -1,2-linked oligomannosidic epitopes in Candida albicans mannan and phospholipomannan

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