Structural studies of the Escherichia coli O-149 O-antigen polysaccharide

Adeyeye, Adenrele; Jansson, Per‐Erik; Lindberg, Bengt; Abaas, Saleem; Svenson, Stefan B.

doi:10.1016/0008-6215(88)80134-4

Cited by 23 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the major (B, C) and the minor rhamnosyl residues (D, E, F), the a-configuration was also supported by the downfield chemical shift observed for H5 of these residues (6 > 3.8) compared to the chemical shift of H5 for P-rhamnopyranosyl residues ( 6~3 . 4 ) [16]. In agreement with the methylation analysis, the HMQC experiment also showed [17], and thereby confirmed the pyranosyl ring form of the galactosyl unit deduced from the ' T -N M R data for this residue (Table 1).…”

Section: ~0 supporting

confidence: 83%

See 1 more Smart Citation

Structural Elucidation of two Polysaccharides Present in the Lipopolysaccharide of a Clinical Isolate of Burkholderia Cepacia

Cérantola

Montrozier

1997

European Journal of Biochemistry

View full text Add to dashboard Cite

Based on the sugar composition, methylation analyses and Smith degradation, supported by NMR spectroscopic analyses and fast-atom-bombardment MS experiments, the lipopolysacharide produced by a clinical isolate of Burkholderia cepacia was shown to contain two distinct polymers, both with linear trisaccharide repeating units ; a major, containing D-rhamnose and D-galactose residues (2 : 1) with the structureand a minor repeating unit, constituted by D-rhamnosyl residues, with the structureKeywards: Burkholderia cepacia; lipopolysaccharide; D-rhamnose; cystic fibrosis; fast-atom-bombardinent MS.Burkholderiu cepacia, previously known as Pseudomonas cepacia, is the type species of the new genus Burkholderia described by Yabuuchi et al. [l]. This bacterium, originally considered as a saprophyte causing soft rot of onions [2], has emerged during the last decade as an important opportunistic pathogen in nosocomial infections and notably as a real threat in patients with cystic fibrosis (CF) due to its association, in some cases, with the cepacia syndrome, i.e. a rapid and fatal decline in pulmonary functions [3-51.In contrast to Pseudomonus ueruginosa, another well-known CF pathogen, little information is available on the molecular basis of the pathogenicity of B. cepucia. Several cellular and extracellular products have been listed as potential virulence factors : these include iron-chelating siderophores, outer membrane proteins, hemolysin, lipases, proteases, exopolysaccharides (EPS) and lipopolysaccharides (LPS) [6-81. Their precise role, however, as pathogenic determinants remains unclear and needs further investigation.In order to distinguish strains of B. cepucia, due to the marked phenotypic variability observed for clinical isolates of this organism, several typing methods have been proposed, notably those based on 0-antigenic polysaccharides of the LPS [9]. The aim of these studies is to understand the epidemiology of B. cepacia and to permit a better biological control of this bacterium. The chemical structures of the 0-polysaccharide repeating units of LPS have been elucidated for many strains of B. cepacia. They were shown to be rather simple, compared with other organisms, with linear disaccharide or trisaccharide repeating Here, we describe the structure of two D-rhamnose-containing LPS-related polymers produced by a clinical isolate of B. cepacia. MATERIALS AND METHODSGrowth conditions, extraction, purification and fractionation of the bacterial LPS. A B. cepacia clinical isolate was obtained from Dr T. L. Pitt (Central Public Health Laboratory, Colindale, London). An 18-h bacterial culture was inoculated on a Pseudomonas isolation agar medium (PTA, Difco) containing 2 % glycerol and incubated for 72 h at 37°C. LPS was extracted from the dry cells by the hot aqueous phenol method [Ill. The aqueous phase was then extensively dialysed against distilled water (4 days at 4°C) to remove phenol and freeze dried. The material was then dissolved in distilled water, digested with nucleases (DNAse I and RNAse A)...

show abstract

Section: ~0 supporting

confidence: 83%

“…4 ) [16]. In agreement with the methylation analysis, the HMQC experiment also showed that the 0 3 of residues B and C were the sites of glycosylation in view of the significant deshielding observed for the chemical shifts of the resonance of the corresponding carbons, 6 79.1 and 78.6 for C3s of residues B and C, respectively.…”

Section: Nmr Studiessupporting

confidence: 62%

Structural Elucidation of two Polysaccharides Present in the Lipopolysaccharide of a Clinical Isolate of Burkholderia Cepacia

Cérantola

Montrozier

1997

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…The structures of B1, B3, B15, D11, D12 and D13 have not been reported earlier and were studied for the first time in this work. A comparison, using the ‘fingerprint’ method, of the 13 C‐NMR spectra of their O antigens with published data of E. coli O149, O167, O112ab, O29, O152 and O150, respectively (Adeyeye et al , 1988; Linnerborg et al , 1997; Olsson et al , 2005; Perepelov et al , 2006a, b, 2008a), showed that the Shigella and E. coli structures are pairwise identical. An exception is the O antigen of D11, whose O unit, as opposed to its E. coli counterpart, contains an O‐acetyl group in a nonstoichiometric amount.…”

Section: Structures Of Shigella O Antigensmentioning

confidence: 99%

Structure and genetics ofShigellaO antigens

et al. 2008

View full text Add to dashboard Cite

This review covers the O antigens of the 46 serotypes of Shigella, but those of most Shigella flexneri are variants of one basic structure, leaving 34 Shigella distinct O antigens to review, together with their gene clusters. Several of the structures and gene clusters are reported for the first time and this is the first such group for which structures and DNA sequences have been determined for all O antigens. Shigella strains are in effect Escherichia coli with a specific mode of pathogenicity, and 18 of the 34 O antigens are also found in traditional E. coli. Three are very similar to E. coli O antigens and 13 are unique to Shigella strains. The O antigen of Shigella sonnei is quite atypical for E. coli and is thought to have transferred from Plesiomonas. The other 12 O antigens unique to Shigella strains have structures that are typical of E. coli, but there are considerably more anomalies in their gene clusters, probably reflecting recent modification of the structures. Having the complete set of structures and genes opens the way for experimental studies on the role of this diversity in pathogenicity.

show abstract

“…The chemical structure of E. coli O149 antigen has been reported ( Fig. 1) (Adeyeye et al 1988). Genes for the biosynthesis of sugars that are also involved in housekeeping functions are generally not duplicated in the O-antigen gene cluster, so only genes for the nucleotide sugar precursor of the sugar rhamnose were expected in the O-antigen gene cluster of S. boydii O1.…”

Section: The O-antigen Gene Cluster Of S Boydii O1mentioning

confidence: 99%

“…Orf6 shared 46% similarity to ExoV of Sinorhizobium meliloti (CAA80360) and PssM of Rhizobium leguminosarum (AAK77315), which are both putative pyruvyltransferases. orf6 is proposed to be (Adeyeye et al 1988). All the genes are transcribed in the direction from galF to gnd.…”

Section: The O-antigen Gene Cluster Of S Boydii O1mentioning

confidence: 99%

Molecular analysis ofShigella boydiiO1 O-antigen gene cluster and its PCR typing

Jiang¹,

Wang²,

Liú³

et al. 2005

Can. J. Microbiol.

View full text Add to dashboard Cite

Shigella is an important human pathogen and is closely related to Escherichia coli. O-antigen is the most variable part of the lipopolysaccharide on the cell surface of Gram-negative bacteria and plays an important role in pathogenicity. The O-antigen gene cluster of S. boydii O1 was sequenced. The putative genes encoding enzymes for rhamnose synthesis, transferases, O-unit flippase, and O-unit polymerase were identified on the basis of homology. The O-antigen gene clusters of S. boydii O1 and E. coli O149, which share the same O-antigen form, were found to have the same genes and organization by adjacent gene PCR assay. Two genes specific for S. boydii O1 and E. coli O149 were identified by PCR screening against E. coli-and Shigella-type strains of the 186 known O-antigen forms and 39 E. coli clinical isolates. A PCR sensitivity of 10 3 to 10 4 CFU/mL overnight culture of S. boydii O1 and E. coli O149 was obtained. S. boydii O1 and E. coli O149 were differentiated by PCR using lacZ-and cadA-based primers.

show abstract

Structural studies of the Escherichia coli O-149 O-antigen polysaccharide

Cited by 23 publications

References 9 publications

Structural Elucidation of two Polysaccharides Present in the Lipopolysaccharide of a Clinical Isolate of Burkholderia Cepacia

Structural Elucidation of two Polysaccharides Present in the Lipopolysaccharide of a Clinical Isolate of Burkholderia Cepacia

Structure and genetics ofShigellaO antigens

Molecular analysis ofShigella boydiiO1 O-antigen gene cluster and its PCR typing

Contact Info

Product

Resources

About