Structural studies of the capsular polysaccharide of Klebsiella type 66

Jansson, Per‐Erik; Lindberg, Bengt; Lōnngren, Jörgen; Ortéga, Carmen; Nimmich, Wolfgang

doi:10.1016/0008-6215(84)85226-x

Cited by 13 publications

(4 citation statements)

References 12 publications

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“…aggregates of Mn(II) with Mn(II)-Mn(II) distances of 10-15 Á were found. 7 Different Mn(II) local environments in hydrated as compared to Mn(II) impregnated onto hydrated and water coordination typical to Mn(II) occupying a negatively charged site suggest that at low Mn(II) contents, 0.1 mol %, the Mn(II) is incorporated into the framework.8 Similar results were obtained for Mn(II) and Co(II) have been incorporated into the chabazite-like structures SAPO-34 and SAPO-44 and the activity and shape selectivity of these materials were tested on methanol dehydration.10 11It was found that both MeAPSO-44 and (Me = Mn,Co) showed better activity than the unsubstituted materials. Moreover, the MeAPSO-44 materials showed considerably better selectivity.…”

Section: Introductionmentioning

confidence: 99%

“…11 In this particular case we can use the modulation from framework 3IP and 27A1 to probe the Mn(II) location. 7 We have also used the "2+1" electron spin echo (ESE) experiment12 to investigate the spatial distribution of the Mn(II). This experiment was specifically designed to study the dipolar interaction between paramagnetic centers which can (1) Wilson, S. T.; Lok, B. M.; Messina, C. A.; Cannan, T. R.; Flanigen, E. M. J.…”

Section: Introductionmentioning

confidence: 99%

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Magnetic resonance studies of SAPO-44 and MnAPSO-44

Olender¹,

Goldfarb²,

Batista³

1993

J. Am. Chem. Soc.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Magnetic resonance studies of SAPO-44 and MnAPSO-44

Olender¹,

Goldfarb²,

Batista³

1993

J. Am. Chem. Soc.

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“…The lactyl group has been found in bacterial polysaccharides, mostly attached to assignments and independently established the inter-residue linkages. The complexity of the deacetylated B62 EPS spectra, a neutral sugar (Kochetkov et al, 1979;Jansson et al, 1984;Osman et al, 1994;Garozzo et al, due to its low molecular mass and to the presence of two repeat-…”

Section: Discussionmentioning

confidence: 99%

Structural investigation of the exopolysaccharide produced by Pseudomonas flavescens strain B62

Cescutti

Toffanin

Fett

et al. 1998

European Journal of Biochemistry

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Pseudomonas flavescens strain B62 (NCPPB 3063) is a recently described bacterium isolated from walnut blight cankers. This strain has been designated as the type strain of a Pseudomonas rRNA group-I species. Strain B62 produced a mixture of two exopolysaccharides, differing in weight average relative molecular mass and composition. Only the most abundant exopolysaccharide (90 % by mass), corresponding to the one with the lower molecular mass, was investigated by use of methylation analysis, partial acid hydrolysis, and NMR spectroscopy. The polysaccharide was depolymerised by the action of the cellulase produced by Penicillum funiculosum and the oligosaccharide obtained, corresponding to the repeating unit, was characterised by NMR spectroscopy and ion-spray mass spectrometry. The repeating unit of the B62 exopolysaccharide is Ac Acwhere X is glucose (75%) or mannose (25 %), and Lac is lactate. The O-acetyl groups are present only on 75% of the repeating units, and they are linked to the C6 of the hexose residues in non-stoichiometric amounts.Keywords : Pseudomonas ; exopolysaccharide; structure ; cellulase ; NMR.Bacterial EPS are of much current interest not only for their In their natural environment bacteria are often found in biofilms sorrounded by a glycocalyx composed primarily of bacte-role in the development of biofilms, but also for other commercial applications. The EPS xanthan and gellan are currently used rial exopolysaccharides (EPS; Costerton et al., 1995). The acidic nature of these polymers is most often due to the presence of as thickeners and gelling agents, respectively, for several food uronic acids, although other acidic substituents (e.g. pyruvate, and non-food applications. Dextran and its derivatives have lactate, succinate, and phosphoric diester groups) may also be applications in the pharmaceutical and fine-chemicals industries present. The polymers are usually heteroglycans with small (Sanford and Baird, 1983). In addition, several EPS are viruoligosaccharide repeating units (Kenne and Lindberg, 1983).lence factors for human, animal and plant bacterial pathogens (Gacesa and Russell, 1990; Leigh and Coplin, 1992 pathogen (Gacesa and Russell, 1990).

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“…Hz, H-3), 3.48 (dd, 1 H, 7= 9.1, 10.7 Hz, H-4), 3.53 (m, 1 H,3 = 6.2, 10.7 Hz H-5), 3.73 (s, 1 H, COOCH3), 3.95 (dd, 1 H, J = 1.7, 3.1 Hz, H-2), 4.39 (q, 1 H, lactyl CH), 4.56 (d, 1 H, J = 1. 7 Hz, H-l); l3C NMR S 19.2 (C-6), 20.3 (lactyl CH3), 53.8 (COOCH3), 56.3 (OCH3), 70.8 (C-5), 71.8 (C-2), 74.6 (C-4), 77.9 (lactyl CH), 82.5 (C-3), 103.6 (C-l). Anal.…”

Section: Methodsmentioning

confidence: 99%

A novel approach for stereochemical analysis of 1-carboxyethyl sugar ethers by NMR spectroscopy

Severn¹,

Richards²

1993

J. Am. Chem. Soc.

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Only one type of TOA site was found in . The Si substitutes mostly for P and in hydrated samples part of the A1 assumes octahedral coordination. The highly crystalline structures of SAPO-44 and MnAPSO-44 break down upon dehydration of calcined samples which underwent prolonged exposure to water vapor. Mn(Il) in MnAPSO-44 with low Mn content (0.07 atom %) assumes octahedral coordination in the calcined hydrated samples.Dehydration to 400 °C changes the Mn(II) coordination to tetrahedral as evident by the considerable decrease in the hyperfme coupling constant from ~90 to 65 G. These results are consistent with Mn(II) situated in the framework. In the hydrated sample the Mn(II) is coordinated to four framework oxygens and two water molecules whereas in the dehydrated sample it is coordinated only to four framework oxygens one of which is part of an OH group. The Mn(II) framework substitution for A1 is supported by relatively weak 27A1 modulation and 31P modulation rather invariant to calcination and hydration.The distribution of the Mn(ll) throughout the sample in both as-synthesized and calcined samples in MnAPSO-44 (0.07 atom %) was found to be inhomogeneous. Only about 15% of the Mn(II) is distributed homogeneously and the rest is situated in areas "rich" in Mn(II).Acknowledgment. This study was supported by funds granted to D.G. through a fellowship program sponsored by the Charles H. Revson Foundation. J.B. gratefully acknowledges the financial support from the National Slovene Board for Science and Technology (RSRDT), Grant No. C2-0538-104. We thank Professor L. Kevan for making refs 8 and 9 available to us prior to publication.

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Structural studies of the capsular polysaccharide of Klebsiella type 66

Cited by 13 publications

References 12 publications

Magnetic resonance studies of SAPO-44 and MnAPSO-44

Magnetic resonance studies of SAPO-44 and MnAPSO-44

Structural investigation of the exopolysaccharide produced by Pseudomonas flavescens strain B62

A novel approach for stereochemical analysis of 1-carboxyethyl sugar ethers by NMR spectroscopy

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