Structural studies of fungal cell-wall polysaccharides from two strains of Talaromyces flavus

Parra, Enrique; Jiménez‐Barbero, Jesús; Bernabé, Manuel; Leal, Juan; Prieto, Alicia; Gómez-Miranda, Begoña

doi:10.1016/0008-6215(94)84294-9

Cited by 35 publications

(15 citation statements)

References 18 publications

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“…The 13 C-NMR spectrum of the acid-degraded polysaccharide is in excellent agreement to reported values of a synthetic (Ogawa and Sasajima 1981) and a yeast α-(1,6)-mannan (Kogan et al 1988). The spectrum (recorded at 70°C) of a similar polysaccharide, which was isolated following Smith degradation of a glucuronorhamnomannan from Ceratocystis stenoceras, also compares favorably with our data (Gorin et al 1977), and one of the two water-soluble cell wall polysaccharides from Talaromyces flavus contains a similar α-(1,6)-mannan (Parra et al 1994). The structural elucidation of the side-chain constituents (including the uronic acid residue), sequence, and linkage pattern requires selective chemical degradations and is a matter of further investigation.…”

Section: Nmr Analysis Of the Heteroglycansupporting

confidence: 77%

“…The side chains attached to this core structure contain at least galactose, glucose, and glucuronic acid (J. Rath, unpublished results). Parra et al (1994) determined the structure of one cell wall polysaccharide from Talaromyces flavus and found the same core structure as described here. This also supports the view that α-1,6-D-mannopyranans are common in cell walls of ascomyceteous fungi.…”

Section: Activation Of Fungal Glycosidases By the Cell-wall Heteroglymentioning

confidence: 77%

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The α- d -mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for β-glucosidase activation

Rath¹,

Messner

Kosma

et al. 1995

Archives of Microbiology

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A heteroglycan responsible for the binding of the enzyme beta-1,4-D-glucosidase (EC 3.2.1.21) to fungal cell walls was isolated from cell walls of the filamentous fungus Trichoderma reesei. The heteroglycan, composed of mannose, galactose, glucose, and glucuronic acid, also activated beta-1,4-D-glucosidase, beta-1,4-D-xylosidase and N-acetyl-beta-1,4-D-glucosaminidase activity in vitro. The structural backbone of this heteroglycan was prepared by acid hydrolysis and gel filtration. The molecular structure of the core of the heteroglycan was determined by NMR studies as a linear alpha-1,6-D-mannan. The mannan core obtained by acid degradation stimulated the beta-glucosidase activity by 90%. Several glycosidases from Aspergillus niger were also activated by the T. reesei heteroglycan. The beta-glucosidase of Trichoderma was activated by mannan from Saccharomyces cerevisiae to a comparable extent.

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Section: Nmr Analysis Of the Heteroglycansupporting

confidence: 77%

Section: Activation Of Fungal Glycosidases By the Cell-wall Heteroglymentioning

confidence: 77%

The α- d -mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for β-glucosidase activation

Rath¹,

Messner

Kosma

et al. 1995

Archives of Microbiology

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“…2) isolated from some species of Penicillium and Talaromyces (Parra et al 1994;Prieto et al 1995;Leal et al 1997). This structure was proposed as a chemotaxonomic marker to delimit species of Penicillium with Talaromyces as their perfect state Leal and Bernabé 1998).…”

Section: Discussionmentioning

confidence: 97%

Heterogeneity of the genus Myrothecium as revealed by cell wall polysaccharides

Ahrazem

Gómez-Miranda

Prieto

et al. 2000

Archives of Microbiology

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The polysaccharides obtained from the alkali-extractable, water-soluble fraction (F1SS) from the cell wall of Myrothecium verrucaria and Myrothecium atroviride were shown to be composed of beta-(1-->6)-galactofuranose fully substituted at O-2 by terminal residues of alpha-glucopyranose and alpha-glucuronic acid. Glucuronic acid was substituted at O-4 by glucopyranose in the Myrothecium species M. inundatum, M. setiramosum, M. prestonii, M. tongaense and M. roridum. The acidic polysaccharides from Phaeostilbella atra (=Myrothecium atrum) and Myrothecium gramineum lacked the backbone of 2,6 di-O-substituted galactofuranose and contained a high amount of O-5-substituted beta-galactofuranose. The structures of the polysaccharides isolated from Myrothecium cinctum and Myrothecium penicilloides were unrelated to each other and to the polysaccharides from the other species analysed. The usefulness of these polysaccharides as a characteristic for delimitation of the genus Myrothecium is discussed.

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“…Under the conditions described in Materials and Methods, the enzyme liberated galactose from E. crustaceum and T. flavus polysaccharides containing f3( 1 ~ 5) galactofuranoside residues 7,9) and Fusarium sp. M7-1 polysaccharide containing 13(1 ~6) galactofuranoside residues.…”

Section: Galactofuranosidase Action On Polysaccharide Containing Galamentioning

confidence: 99%

Isolation and Characterization of a Novel Endo-β-galactofuranosidase fromBacillussp.

Nahrowi

Fujinaga

Tabuchi

et al. 1995

Bioscience, Biotechnology, and Biochemistry

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Structural studies of fungal cell-wall polysaccharides from two strains of Talaromyces flavus

Cited by 35 publications

References 18 publications

The α- d -mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for β-glucosidase activation

The α- d -mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for β-glucosidase activation

Heterogeneity of the genus Myrothecium as revealed by cell wall polysaccharides

Isolation and Characterization of a Novel Endo-β-galactofuranosidase fromBacillussp.

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