Structural Stabilization of Granular Sludge by Addition of Calcium Ions into Aerobic Bioreactors

Morais, Ismarley Lage Horta; Silva, Cláudio; Zanúncio, José Cola; Zanuncio, Antônio José Vinha

doi:10.15376/biores.13.1.176-191

Cited by 6 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Morais et al (2018) and Hao et al (2016), the addition of calcium ions enhanced the uniformity of the structure of the granules and increased their mechanical strength. Ca 2+ ions have a positive effect on the process of microbial attachment that influences the settling velocity of the granules.…”

Section: Resultsmentioning

confidence: 99%

Chemical and Process Engineering

Kończak,

Miksch

2020

View full text Add to dashboard Cite

In this study, we performed the qualitative analysis of exoproteins during granule formation in the presence or in the absence of cations. The staining of thin granule cryosections showed that nucleic acids, proteins, polysaccharides and calcium cations were the dominant components of the granules. Proteins are the structural components associated with calcium ions. We determined changes in the proteomic profile and tightly bound extracellular polymeric substances (EPS) of the slime. The exopolymeric matrix containing the proteins was extracted using the Dowex resin method. Proteomic profile was analysed by SDS-PAGE method (sodium dodecyl sulphate polyacrylamide gel electrophoresis) using Coomassie blue staining in the samples of the aerobic granule matrix formed in the presence of multivalent cations and compared with that of the aerobic granules cultivated without cations. The results indicate that the granule matrix is predominantly composed of large and complex proteins that are tightly bound within the granular structure. The tightly bound extracellular polymeric substances (TB-EPS) may play a role in improved mechanical stability of aerobic granules. In the supernatant fraction of the sludge, only a small amount of free proteins in the medium molecular mass range was detected. The protein with high molecular mass (≥ 116 kDa) produced in the reactors with added Ca 2+ . Ca 2+ had a considerable regulatory influence on production of extracellular proteins during aerobic granulation.

show abstract

Section: Resultsmentioning

confidence: 99%

Chemical and Process Engineering

Kończak,

Miksch

2020

View full text Add to dashboard Cite

show abstract

“…In most of the previous studies, Ca 2+ is always the focus, as it is deemed to have the most vital role in the granulation process. 64,65 Several advantages such as the improvement of sludge settleability and formation of aerobic granules have been reported after the addition of Ca 2+ . 65,66 However, the higher concentration of Mg 2+ and Fe 3+ measured in all the granular sludge samples during M1, M2 and M3 disprove the previous findings.…”

Section: Multivalent Cationsmentioning

confidence: 99%

Simultaneous nitrogen and phosphorus removal from domestic wastewater in an aerobic granulation system operated at different anaerobic–aerobic durations

Aris

Zain

et al. 2022

Environ. Sci.: Water Res. Technol.

View full text Add to dashboard Cite

show abstract

“…An inadequate concentration of Ca 2+ can hinder granulation and lead to granule disintegration ( Grotenhuis et al., 1991 ), while an excessively high concentration of Ca 2+ can severely suppress microbial activity and result in sludge mineralization ( Wang et al., 2018 ; Xia et al., 2016 ). Therefore, maintaining a stable Ca 2+ concentration in anaerobic granular sludge is crucial for efficient reactor operation ( Morais et al., 2018 ; Zhang et al., 2021a , 2021b ).…”

Section: Introductionmentioning

confidence: 99%

The neglected ammonia leaching calcium in anaerobic granular sludge

Wen,

Li,

Tian

et al. 2023

Water Research X

View full text Add to dashboard Cite

Structural Stabilization of Granular Sludge by Addition of Calcium Ions into Aerobic Bioreactors

Cited by 6 publications

References 0 publications

Chemical and Process Engineering

Chemical and Process Engineering

Simultaneous nitrogen and phosphorus removal from domestic wastewater in an aerobic granulation system operated at different anaerobic–aerobic durations

The neglected ammonia leaching calcium in anaerobic granular sludge

Contact Info

Product

Resources

About