Structural Stability Effects on Adsorption of Bacteriophage T4 Lysozyme to Colloidal Silica

Activity losses among T4 lysozyme charge variants after adsorption to colloidal silica

“…For example, K135E exhibited the highest activity but the most extensive conformational change. This appears at first inconsistent with earlier findings reported for T4L stability variants, where enzymes of lower stability were observed to undergo more extensive conformational change and experience greater loss in enzymatic activity (Bower et al, 1998;Tian et al, 1998). This is probably a result of variant-specific differences in adsorption mechanism.…”

“…The ␣-helix content in each case was estimated using the ellipticity recorded at 222 nm, according to methods described by Kondo et al (1992). By comparing the secondary structures of each T4L variant before and after the addition of particles, it was possible to calculate the percentage of ␣-helix lost after adsorption (Tian et al, 1998).…”

Details of the Partial Unfolding of T4 Lysozyme on Quartz Using Site‐Directed Spin Labeling

“…[15,16]). Surface-sensitive variants of vibrational (ATR-IR, Raman, SFG) spectroscopy, [17][18][19][20][21] fluorescence spectroscopy, [22][23][24] circular dichroism, [25][26][27] surface force measurements [28] and microscopy, [29] and neutron reflectivity [30,31] have been used to study structural changes of adsorbed proteins, providing global statements on changes in secondary and tertiary structure or information on special chromophores. However, detailed information on the level of the backbone fold could not be obtained.…”

Anti-thrombogenicity by Layer-by-Layer

“…For those reasons, there is a need to control thrombus formation on medical equipment. Only a few findings have been reported regarding the effect of surface morphology on small proteins such as lysozyme and cytochrome 1,2 ; the majority of reports have focused on the effects of surface chemistry 3,4,5,6 . The surfaces can be categorized into four main types, namely heparinized or biological surfaces 7 , electrically charged surfaces 8 , inert surfaces 9,10 and solution-perfused surfaces 11 .…”