Structural Similarity Image Analysis for Detection of Adenosine and Dopamine in Fast-Scan Cyclic Voltammetry Color Plots

Puthongkham, Pumidech; Rocha, Julian; Borgus, Jason R; Ganesana, Mallikarjunarao; Wang, Ying; Chang, Yuanyu; Gahlmann, Andreas; Venton, B. Jill

doi:10.1021/acs.analchem.0c01214

Cited by 22 publications

(17 citation statements)

References 50 publications

(139 reference statements)

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“…Figure C shows a typical 3 min duration false-color plot of in vivo data. An automated image processing program is used to identify ADO and DA transients from the color plot . Transients identified by the automated algorithm are circled in blue for ADO and red for DA.…”

Section: Results and Discussionmentioning

confidence: 99%

Spontaneous Adenosine and Dopamine Cotransmission in the Caudate-Putamen Is Regulated by Adenosine Receptors

Borgus

Wang

DiScenza

et al. 2021

ACS Chem. Neurosci.

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Transient changes in adenosine and dopamine have been measured in vivo, but no studies have examined if these transient changes occur simultaneously. In this study, we characterized spontaneous adenosine and dopamine transients in anesthetized mice, examining coincident release in the caudateputamen for the first time. We found that in C57B mice, most of the dopamine transients (77%) were coincident with adenosine, but fewer adenosine transients (12%) were coincident with a dopamine transient. On average, the dopamine transient started 200 ms before its coincident adenosine transient, so they occurred concurrently. There was a positive correlation (r = 0.7292) of adenosine and dopamine concentrations during coincident release. ATP is quickly broken down to adenosine in the extracellular space, and the coincident events may be due to corelease, where dopaminergic vesicles are packaged with ATP, or cotransmission, where ATP is packaged in different vesicles released simultaneously with dopamine. The high frequency of adenosine transients compared to that of dopamine transients suggests that adenosine is also released from nondopaminergic vesicles. We investigated how A 1 and A 2A adenosine receptors regulate adenosine and dopamine transients using A 1 and A 2A KO mice. In A 1 KO mice, the frequency of adenosine and dopamine transients increased, while in A 2A KO mice, the frequency of adenosine alone increased. Adenosine receptors modulate coincident transients and could be drug targets to modulate both dopamine and adenosine release. Many spontaneous dopamine transients have coincident adenosine release, and regulating adenosine and dopamine cotransmission could be important for designing treatments for dopamine diseases, such as Parkinson's or addiction.

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Section: Results and Discussionmentioning

confidence: 99%

Spontaneous Adenosine and Dopamine Cotransmission in the Caudate-Putamen Is Regulated by Adenosine Receptors

Borgus

Wang

DiScenza

et al. 2021

ACS Chem. Neurosci.

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“…Other considerations are the processing time for data analysis and errors due to bias. More recently, a novel image analysis technique was applied to color plots to successfully discriminate simultaneous DA and adenosine signals [ 230 ]. There are open opportunities to apply newer signal processing techniques to optimize NT detection with diamond electrodes, particularly in the in vivo setting.…”

Section: Opportunities For Optimizationmentioning

confidence: 99%

Next-Generation Diamond Electrodes for Neurochemical Sensing: Challenges and Opportunities

et al. 2021

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Carbon-based electrodes combined with fast-scan cyclic voltammetry (FSCV) enable neurochemical sensing with high spatiotemporal resolution and sensitivity. While their attractive electrochemical and conductive properties have established a long history of use in the detection of neurotransmitters both in vitro and in vivo, carbon fiber microelectrodes (CFMEs) also have limitations in their fabrication, flexibility, and chronic stability. Diamond is a form of carbon with a more rigid bonding structure (sp3-hybridized) which can become conductive when boron-doped. Boron-doped diamond (BDD) is characterized by an extremely wide potential window, low background current, and good biocompatibility. Additionally, methods for processing and patterning diamond allow for high-throughput batch fabrication and customization of electrode arrays with unique architectures. While tradeoffs in sensitivity can undermine the advantages of BDD as a neurochemical sensor, there are numerous untapped opportunities to further improve performance, including anodic pretreatment, or optimization of the FSCV waveform, instrumentation, sp2/sp3 character, doping, surface characteristics, and signal processing. Here, we review the state-of-the-art in diamond electrodes for neurochemical sensing and discuss potential opportunities for future advancements of the technology. We highlight our team’s progress with the development of an all-diamond fiber ultramicroelectrode as a novel approach to advance the performance and applications of diamond-based neurochemical sensors.

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“…The reported 5-HT sensitivity was 5 nAµM −1 , compared to a typical bare CFM sensitivity of 1 nAµM −1 [ 58 ]. Additional FSCV waveforms to selectively detect histamine, adenosine, melatonin, hydrogen peroxide and others have been developed [ 59 , 60 , 61 , 62 ].…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

Recent Advances in In Vivo Neurochemical Monitoring

Tan

Robbins

et al. 2021

Micromachines

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The brain is a complex network that accounts for only 5% of human mass but consumes 20% of our energy. Uncovering the mysteries of the brain’s functions in motion, memory, learning, behavior, and mental health remains a hot but challenging topic. Neurochemicals in the brain, such as neurotransmitters, neuromodulators, gliotransmitters, hormones, and metabolism substrates and products, play vital roles in mediating and modulating normal brain function, and their abnormal release or imbalanced concentrations can cause various diseases, such as epilepsy, Alzheimer’s disease, and Parkinson’s disease. A wide range of techniques have been used to probe the concentrations of neurochemicals under normal, stimulated, diseased, and drug-induced conditions in order to understand the neurochemistry of drug mechanisms and develop diagnostic tools or therapies. Recent advancements in detection methods, device fabrication, and new materials have resulted in the development of neurochemical sensors with improved performance. However, direct in vivo measurements require a robust sensor that is highly sensitive and selective with minimal fouling and reduced inflammatory foreign body responses. Here, we review recent advances in neurochemical sensor development for in vivo studies, with a focus on electrochemical and optical probes. Other alternative methods are also compared. We discuss in detail the in vivo challenges for these methods and provide an outlook for future directions.

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Structural Similarity Image Analysis for Detection of Adenosine and Dopamine in Fast-Scan Cyclic Voltammetry Color Plots

Cited by 22 publications

References 50 publications

Spontaneous Adenosine and Dopamine Cotransmission in the Caudate-Putamen Is Regulated by Adenosine Receptors

Spontaneous Adenosine and Dopamine Cotransmission in the Caudate-Putamen Is Regulated by Adenosine Receptors

Next-Generation Diamond Electrodes for Neurochemical Sensing: Challenges and Opportunities

Recent Advances in In Vivo Neurochemical Monitoring

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