Structural rearrangements of chromosome Nr 8 involving 8q12?a primary event in pleomorphic ademona of the parotid gland

Bullerdiek, J.; Raabe, G; Bartnitzke, Sabine; Böschen, Christa; Schloot, W.

doi:10.1007/bf00123166

Cited by 32 publications

(11 citation statements)

References 11 publications

(10 reference statements)

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“…Cytogenetically, pleomorphic adenomas of the salivary glands are well characterized, with several hundreds already karyotyped (7,8). Four major cytogenetic subgroups can be distinguished (9).…”

Section: Introductionmentioning

confidence: 99%

Upregulation of Igf and Wnt signalling associated genes in pleomorphic adenomas of the salivary glands in PLAG1 transgenic mice

Declercq

Dyck²,

Damme³

et al. 2008

Int J Oncol

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Abstract. The Pleomorphic adenoma gene 1 (PLAG1) is involved in various human neoplasias, including pleomorphic adenomas of the salivary glands. Moreover, the oncogenic role of PLAG1 was clearly demonstrated in two independent PLAG1 transgenic mouse founders, in which PLAG1 expression could be targeted to different tissues using the Cre/loxP system. MMTV-Cre-mediated targeted overexpression of PLAG1 in the salivary glands of double transgenic offspring mice, referred to as P1-MCre and P2-MCre mice, induced pleomorphic adenomas in this organ. Igf2, a genuine PLAG1 target gene, was highly upregulated in those tumours as well as in human pleomorphic adenomas of the salivary glands. These and previous observations in other PLAG1-induced tumours e.g. breast adenomyoepitheliomas emphasize the importance of Igf upregulation in such tumours. In this study, further evidence for the role of Igf2 in PLAG1-induced tumourigenesis, is reported. Inactivation of Igf2 in P1-MCre mice leads to a significant delay in tumour development. Since tumour development is not fully abrogated by inactivation of Igf2, other signalling pathways are likely to contribute to PLAG1-induced tumourigenesis as well. Further studies revealed that several genes such as H19, Dlk1, Gtl2, Igfbp2, Igfbp3 and genes involved in Wnt signalling, such as Wnt6, Cyclin D1 and ß-catenin are upregulated in P1-MCre mice in which Igf2 is inactivated. In conclusion, we clearly demonstrate upregulation of several genes associated with Igf and Wnt signalling in PLAG1-induced pleomorphic adenomas. Furthermore, inactivation of Igf2 does not affect upregulation of genes associated with Wnt signalling, which might suggest that both signalling pathways are involved.

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Section: Introductionmentioning

confidence: 99%

Upregulation of Igf and Wnt signalling associated genes in pleomorphic adenomas of the salivary glands in PLAG1 transgenic mice

Declercq

Dyck²,

Damme³

et al. 2008

Int J Oncol

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“…As reported by Mark et al (1980), Stenman (1983), and Rullerdiek et al (1987a), about 50% of all PSA have clonal aberrations involving chromosome 8 or 12 with breakpoints in 8q12 and 12q13-15. Bullerdiek et al (1987b) therefore proposed a cytogenetic subdivision of PSA into three major subgroups: 1) tumors with rearrangements in 8q12 2) tumors with rearrangements in 12q13-15; and 3) tumors with a normal karyotype.…”

Section: Introductionmentioning

confidence: 99%

Pleomorphic adenoma cells vary in their susceptibility to SV40 transformation depending on the initial karyotype

Kazmierczak

Thode²,

Bartnitzke

et al. 1992

Genes Chromosomes & Cancer

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Chromosomal aberrations involving 8q12 or 12q13-15 characterize two cytogenetic subgroups of salivary gland pleomorphic adenomas. As the tumors of the two groups differ in their clinical and histologic characteristics, we decided to determine their susceptibility to SV40 transformation. We transfected cell cultures from 13 adenomas with aberrations involving 8q12 and from seven adenomas with involvement of 12q13-15 using an SV40 plasmid coding for the early region of the viral genome. Whereas all cultures with aberrations of 12q13-15 showed transformed foci, only 4 of the 13 cultures with 8q12 abnormalities showed foci of transformed cells. We also observed a much higher immortalization rate in the first group (3/7 vs. 1/13). All successfully transformed tumor cell cultures showed a relatively stable karyotype in the pre-crisis stage and a high mitotic index, were T-antigen positive, and had an extended life span in vitro.

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“…1988). These pri mary aberrations probably act as an initiating event for tumorigenesis by activating oncogenic DNA sequences, as discussed by Bullerdiek et al (1987c). Thus, the molecular characterization of the breakpoint region 12ql3-+ql5 will be very helpful for un derstanding of the initiation of these tumors.…”

mentioning

confidence: 99%

In vitro transformation by the SV40 ‘early region’ of cells from a human benign salivary gland tumor with a12q13→q15 rearrangement

Kazmierczak

Bartnitzke²,

Hartl³

et al. 1990

Cytogenet Genome Res

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Cells from a salivary gland pleomorphic adenoma with a reciprocal translocation, t(8; 12)(q22;q 14), were transformed by transfection with a plasmid containing the SV40 “early region.” Compared with nontransformed adenoma cells, the cells expressed large-T antigen, had a higher proliferative capacity, showed a drastically increased or even unlimited growth potential in vitro, and formed colonies in soft agar. The amount of cellular material produced in this way should make possible many experiments at the molecular level that could not be performed before.

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Structural rearrangements of chromosome Nr 8 involving 8q12?a primary event in pleomorphic ademona of the parotid gland

Cited by 32 publications

References 11 publications

Upregulation of Igf and Wnt signalling associated genes in pleomorphic adenomas of the salivary glands in PLAG1 transgenic mice

Upregulation of Igf and Wnt signalling associated genes in pleomorphic adenomas of the salivary glands in PLAG1 transgenic mice

Pleomorphic adenoma cells vary in their susceptibility to SV40 transformation depending on the initial karyotype

In vitro transformation by the SV40 ‘early region’ of cells from a human benign salivary gland tumor with a12q13→q15 rearrangement

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