Structural proteome of human colostral fat globule membrane proteins

Fortunato, Donatella; Giuffrida, Maria Gabriella; Cavaletto, Maria; Garoffo, Lorenza Perono; Dellavalle, G.; Napolitano, Lorenzo; Giunta, Carlo; Fabris, C; Bertino, Enrico; Coscia, Alessandra; Conti, Amedeo

doi:10.1002/pmic.200300367

Cited by 91 publications

(84 citation statements)

References 19 publications

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“…Several earlier studies have collected proteomic data from the mammary tissue of lactating animals (1,6,14,16,18,48,50,60). None have studied mammary mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle

Hadsell

Olea

Wei

et al. 2011

Physiological Genomics

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Hadsell DL, Olea W, Wei J, Fiorotto ML, Matsunami RK, Engler DA, Collier RJ. Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle. Physiol Genomics 43: 271-285, 2011. First published December 28, 2010 doi:10.1152/physiolgenomics.00133.2010.-The regulation of mitochondrial biogenesis and function in the lactating mammary cell is poorly understood. The goal of this study was to use proteomics to relate temporal changes in mammary cell mitochondrial function during lactation to changes in the proteins that make up this organelle. The hypothesis tested was that changes in mammary cell mitochondrial biogenesis and function during lactation would be accounted for by coordinated changes in the proteins of the electron transport chain and that some of these proteins might be linked by their expression patterns to PPARGC1␣ and AMP kinase. The mitochondrial proteome was studied along with markers of mitochondrial biogenesis and function in mammary tissue collected from mice over the course of a single prolonged lactation cycle. Mammary tissue concentrations of AMP and ADP were increased (P Ͻ 0.05) during early lactation and then declined with prolonged lactation. Similar changes were also observed for mitochondrial ATP synthesis activity, mitochondrial mass and DNA copy number. Analysis of the mammary cell mitochondrial proteome identified 244 unique proteins. Of these, only two proteins of the electron transport chain were found to increase during early lactation. In contrast, coordinated changes in numerous electron transport chain proteins were observed both during mid-and late lactation. There were six proteins that could be directly linked to PPARGC1␣ through network analysis. Abundance of PPARGC-1␣ and phosphorylation of AMP kinase was highest on day 2 postpartum. The results suggest that the increases in mammary mitochondria ATP synthesis activity during early lactation results from changes in only a limited number proteins. In addition, decreases in a handful of proteins linked to lipid oxidation could be temporally linked to decreases in PPARGC1␣ and phospho-AMP kinase suggesting potential roles for these proteins in coordinating mammary gland metabolism during early lactation. mitochondria; proteome; adenosine 5=-triphosphate

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“…Several earlier studies have collected proteomic data from the mammary tissue of lactating animals (1,6,14,16,18,48,50,60). None have studied mammary mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle

Hadsell

Olea

Wei

et al. 2011

Physiological Genomics

View full text Add to dashboard Cite

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“…Isoelectric focusing (IEF) and SDS-PAGE were carried out as described elsewhere (9). Gels were automatically stained using the Processor Plus (Amersham Biosciences, Uppsala, Sweden) with colloidal homemade Coomassie Blue (19).…”

Section: Two-dimensional Electrophoresismentioning

confidence: 99%

“…Passing through the mammary secretory cell, fat is segregated in vesicles enveloped by the apical membrane ofthe secretory cell itselfbefore secretion, giving rise to fat globules (7)(8). The main proteins associated with human MFGM have been recently identified by combining effective solubilization procedures, 2-DE and mass spectrometry techniques (9). So far, very little has been reported about pretenn milk MFGM proteins.…”

mentioning

confidence: 99%

Proteolysis of Milk Fat Globule Membrane Proteins in Preterm Milk: A Transient Phenomenon with a Possible Biological Role?

Giuffrida

Cavaletto

Lamberti

et al. 2008

Int J Immunopathol Pharmacol

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Milk fat globule membrane (MFGM) proteins constitute a milk fraction currently of great interest, as they appear to significantly contribute to milk protective role. We investigated these proteins in human preterm colostrum and milk. For the former we found a peculiar 2-DE pattern, with a spot concentration at low molecular weight, which mass spectrometry analysis showed to be fragments belonging to some MFGM proteins with a well-known biological and especially immunological role: lactadherin, membrane-associated lactoferrin, butyrophilin, clusterin and heavy-chain immunoglobulin. Since we were able to rule out protease activity after specimen collection, we hypothesize the localization of the proteolytic enzymes in the alveolar cell membranes of the mammary gland. This mechanism is probably under hormonal control and the unexpected advent of preterm delivery would not allow hormonal conditions typical of lactation to occur immediately, causing a delay in enzymatic inhibition. This hypothesis is supported by some of our results, picturing a peculiar transient phenomenon of adaptation of the mammary-gland-membrane proteins after preterm delivery. Further studies will be required to verify whether the presence of protein fragments exerts a specific biological and immuno-defensive role in preterm infants, thus adding evidence to the outstanding biological role and benefits of mother's own milk in feeding preterm infants.

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“…Compared to MFGM very little literature is available on colostrum fat globule membranes (CFGM). The limited work carried out so far on CFGM, from human and bovine sources, has focussed on the structural and developmental proteomes [26,27,28]. In the present study the FGMs were isolated from buffalo milk and colostrum.…”

Section: Introductionmentioning

confidence: 99%

Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

2017

J App Biol Biotech

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Colostrum is the first liquid food for the new born baby, secreted after the parturition. After few days of maturation the colostrum turns to milk. The cream or fat fraction of milk consists of fat droplets composed primarily of triacylglycerols that are secreted from the apical surface of the mammary cells surrounded by cellular thin membranes membrane, the fat globule membrane (FGM), formed by proteins which have been suggested to be cholesterolemia-lowering factors, inhibitors of cancer cell growth, vitamin binders, antioxidant, bactericidal, suppressors of multiple sclerosis. FGM was isolated from buffalo colostrum and milk, the fat content was > 2-fold higher in the former than in the latter. The colostrum fat globule membrane (CFGM) fraction also showed significantly higher protein content than milk fat globule membrane (MFGM) extract. Further, the SDS-PAGE separation of the total proteins from the CFGM and MFGM showed differential banding pattern. Antioxidative property of the two FGM extracts was deliberated based on their free radical scavenging ability and reducing power. CFGM was found to exhibit a relatively higher antioxidant and reductive capacity than the MFGM fractions. The results obtained in the present study showcases the superior beneficial health effects of the CFGM over the MFGM.

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Structural proteome of human colostral fat globule membrane proteins

Cited by 91 publications

References 19 publications

Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle

Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle

Proteolysis of Milk Fat Globule Membrane Proteins in Preterm Milk: A Transient Phenomenon with a Possible Biological Role?

Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

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